YDR254W / CHL4

Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR254W / CHL4

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:4.52|P-value:2.25E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:3.15|P-value:6.90E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.27|P-value:7.35E-6 ID:SGTC_263|Compound:alizarin|FD-Score:3.06|P-value:9.53E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.18|P-value:6.36E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.56|P-value:1.81E-6 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.11|P-value:8.15E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.02|P-value:1.78E-7 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:4.16|P-value:1.20E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:3.90|P-value:3.79E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.48|P-value:2.04E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:-3.37|P-value:3.12E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.32|P-value:3.74E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.43|P-value:2.46E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.52|P-value:1.80E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.06|P-value:9.42E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.69|P-value:9.14E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.30|P-value:4.12E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:4.12|P-value:1.47E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.39|P-value:2.86E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-5.23|P-value:5.51E-8 ID:SGTC_1664|Compound:st014217|FD-Score:-3.64|P-value:1.11E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.44|P-value:2.38E-4 ID:SGTC_2040|Compound:5100729|FD-Score:5.02|P-value:1.77E-7 ID:SGTC_2255|Compound:7941087|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2258|Compound:7960471|FD-Score:-3.32|P-value:3.78E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.69|P-value:9.02E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-3.45|P-value:2.29E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.06|P-value:9.39E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.75|P-value:7.05E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.34|P-value:5.45E-6 ID:SGTC_2769|Compound:toremifene|FD-Score:3.31|P-value:4.00E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.64|P-value:1.08E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.13|P-value:7.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.22|P-value:5.48E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-3.22|P-value:5.45E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.50|P-value:1.96E-4 ID:SGTC_27|Compound:0485-0001|FD-Score:4.52|P-value:2.25E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:3.15|P-value:6.90E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.27|P-value:7.35E-6 ID:SGTC_263|Compound:alizarin|FD-Score:3.06|P-value:9.53E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.18|P-value:6.36E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.56|P-value:1.81E-6 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.11|P-value:8.15E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.02|P-value:1.78E-7 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:4.16|P-value:1.20E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:3.90|P-value:3.79E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.48|P-value:2.04E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:-3.37|P-value:3.12E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.32|P-value:3.74E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.43|P-value:2.46E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.52|P-value:1.80E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.06|P-value:9.42E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.69|P-value:9.14E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.30|P-value:4.12E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:4.12|P-value:1.47E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.39|P-value:2.86E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-5.23|P-value:5.51E-8 ID:SGTC_1664|Compound:st014217|FD-Score:-3.64|P-value:1.11E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.44|P-value:2.38E-4 ID:SGTC_2040|Compound:5100729|FD-Score:5.02|P-value:1.77E-7 ID:SGTC_2255|Compound:7941087|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2258|Compound:7960471|FD-Score:-3.32|P-value:3.78E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.69|P-value:9.02E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-3.45|P-value:2.29E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.06|P-value:9.39E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.75|P-value:7.05E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.34|P-value:5.45E-6 ID:SGTC_2769|Compound:toremifene|FD-Score:3.31|P-value:4.00E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.64|P-value:1.08E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.17|P-value:6.46E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.13|P-value:7.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.22|P-value:5.48E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-3.22|P-value:5.45E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.50|P-value:1.96E-4

Top fitness defect scores for YDR254W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2040 5100729 5.02 123.0 µM 1.77E-7 20 2.59E-7
2 SGTC_514 4466-0038 5.02 2.6 nM 1.78E-7 cell wall 105 2.60E-7
3 SGTC_27 0485-0001 4.52 32.6 µM 2.25E-6 RPP1 & pyrimidine depletion 2 3.07E-6
4 SGTC_2697 mebhydrolin 4.34 72.4 µM 5.45E-6 NEO1 55 7.25E-6
5 SGTC_212 3807-4682 4.27 38.8 µM 7.35E-6 tubulin folding & SWR complex 54 9.71E-6
6 SGTC_563 tetrahydrosecaminediol 4.16 614.0 µM 1.20E-5 amide catabolism 15 1.56E-5
7 SGTC_1389 3405-0187 4.12 19.0 µM 1.47E-5 10 1.91E-5
8 SGTC_606 k015-0008 3.90 38.6 µM 3.79E-5 14 4.79E-5
9 SGTC_2690 usnic acid 3.75 100.0 µM 7.05E-5 32 8.76E-5
10 SGTC_1243 0416-0003 3.69 5.7 µM 9.14E-5 PDR1 37 1.13E-4
11 SGTC_1134 3477-0009 3.52 42.5 µM 1.80E-4 61 2.18E-4
12 SGTC_3311 9131146 3.50 2.9 µM 1.96E-4 cell wall 140 2.37E-4
13 SGTC_1951 sophoraflavanone G 3.44 9.2 µM 2.38E-4 RNA processing & uracil transport 51 2.86E-4
14 SGTC_2255 7941087 3.35 197.4 µM 3.45E-4 50 4.11E-4
15 SGTC_1078 idebenone 3.32 36.1 µM 3.74E-4 Golgi 69 4.44E-4
16 SGTC_2769 toremifene 3.31 3.7 µM 4.00E-4 45 4.74E-4
17 SGTC_1351 1496-0003 3.30 46.5 µM 4.12E-4 37 4.89E-4
18 SGTC_360 0957-0399 3.18 1.0 µM 6.36E-4 cell wall 84 7.45E-4
19 SGTC_2920 7982904 3.17 15.8 µM 6.46E-4 48 7.57E-4
20 SGTC_150 3970-0969 3.15 77.2 µM 6.90E-4 109 8.07E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 1.60E-31 YDR318W MCM21 hom Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
0.174 2.67E-24 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.148 6.65E-18 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.147 9.50E-18 YOR195W SLK19 hom Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
0.141 2.66E-16 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.140 3.41E-16 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.136 2.64E-15 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.128 1.09E-13 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.121 1.77E-12 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.118 6.31E-12 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.117 8.50E-12 YDR117C TMA64 hom Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
0.117 1.07E-11 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.116 1.58E-11 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.114 3.43E-11 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.106 7.54E-10 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci