YDR297W / SUR2

Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR297W / SUR2

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:4.66|P-value:4.79E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:3.67|P-value:2.47E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.15|P-value:4.08E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-5.49|P-value:7.90E-8 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.36|P-value:6.46E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.86|P-value:1.97E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.03|P-value:7.41E-7 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.03|P-value:5.81E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:4.08|P-value:5.28E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.38|P-value:6.66E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.95|P-value:1.29E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:12.00|P-value:1.51E-30 ID:SGTC_463|Compound:ikarugamyin|FD-Score:29.40|P-value:1.26E-172 ID:SGTC_470|Compound:aa-861|FD-Score:3.49|P-value:4.56E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.11|P-value:4.84E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:4.66|P-value:4.78E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:-3.38|P-value:6.00E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-4.41|P-value:1.24E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.29|P-value:8.02E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.26|P-value:9.97E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.97|P-value:8.10E-5 ID:SGTC_1019|Compound:4262-0212|FD-Score:-3.64|P-value:2.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.22|P-value:3.05E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.27|P-value:9.75E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.17|P-value:3.70E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.95|P-value:8.72E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:-3.33|P-value:7.09E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:-4.02|P-value:6.02E-5 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.60|P-value:3.11E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.46|P-value:4.69E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.58|P-value:2.74E-13 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.93|P-value:8.35E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.47|P-value:4.94E-4 ID:SGTC_1656|Compound:st012921|FD-Score:3.41|P-value:6.14E-4 ID:SGTC_1687|Compound:st020778|FD-Score:24.60|P-value:2.23E-121 ID:SGTC_1712|Compound:st032288|FD-Score:-4.34|P-value:1.66E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.27|P-value:8.61E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.13|P-value:3.83E-5 ID:SGTC_2073|Compound:5212005|FD-Score:3.48|P-value:4.80E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.36|P-value:6.50E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.07|P-value:5.48E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-3.81|P-value:1.32E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.76|P-value:2.71E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.95|P-value:1.13E-6 ID:SGTC_2265|Compound:7929201|FD-Score:3.35|P-value:7.40E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-5.10|P-value:5.46E-7 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.25|P-value:2.66E-5 ID:SGTC_2521|Compound:angolensin|FD-Score:3.59|P-value:3.24E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:9.35|P-value:2.96E-19 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.62|P-value:4.50E-8 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:3.70E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:34.10|P-value:5.96E-232 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-4.47|P-value:9.38E-6 ID:SGTC_2683|Compound:piperine|FD-Score:3.50|P-value:4.50E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.51|P-value:8.19E-6 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.00|P-value:6.49E-5 ID:SGTC_2793|Compound:7325131|FD-Score:7.41|P-value:8.97E-13 ID:SGTC_2796|Compound:7949634|FD-Score:-4.12|P-value:4.11E-5 ID:SGTC_2856|Compound:9022925|FD-Score:-4.22|P-value:2.76E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-6.76|P-value:5.39E-11 ID:SGTC_2948|Compound:9070189|FD-Score:3.80|P-value:1.55E-4 ID:SGTC_3000|Compound:9075062|FD-Score:4.16|P-value:3.85E-5 ID:SGTC_3003|Compound:9075612|FD-Score:5.20|P-value:3.94E-7 ID:SGTC_3071|Compound:9117417|FD-Score:-3.90|P-value:9.53E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-8.02|P-value:9.12E-15 ID:SGTC_3130|Compound:9127414|FD-Score:4.50|P-value:9.67E-6 ID:SGTC_3132|Compound:9126706|FD-Score:-3.77|P-value:1.57E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.09|P-value:5.14E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.91|P-value:1.55E-6 ID:SGTC_8|Compound:0354-0001|FD-Score:4.66|P-value:4.79E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:3.67|P-value:2.47E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.15|P-value:4.08E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-5.49|P-value:7.90E-8 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.36|P-value:6.46E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.86|P-value:1.97E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.03|P-value:7.41E-7 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.03|P-value:5.81E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:4.08|P-value:5.28E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.38|P-value:6.66E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.95|P-value:1.29E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:12.00|P-value:1.51E-30 ID:SGTC_463|Compound:ikarugamyin|FD-Score:29.40|P-value:1.26E-172 ID:SGTC_470|Compound:aa-861|FD-Score:3.49|P-value:4.56E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.11|P-value:4.84E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:4.66|P-value:4.78E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:-3.38|P-value:6.00E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-4.41|P-value:1.24E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.29|P-value:8.02E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.26|P-value:9.97E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.97|P-value:8.10E-5 ID:SGTC_1019|Compound:4262-0212|FD-Score:-3.64|P-value:2.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.22|P-value:3.05E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.27|P-value:9.75E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.17|P-value:3.70E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.95|P-value:8.72E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:-3.33|P-value:7.09E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:-4.02|P-value:6.02E-5 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.60|P-value:3.11E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.46|P-value:4.69E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.58|P-value:2.74E-13 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.93|P-value:8.35E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.47|P-value:4.94E-4 ID:SGTC_1656|Compound:st012921|FD-Score:3.41|P-value:6.14E-4 ID:SGTC_1687|Compound:st020778|FD-Score:24.60|P-value:2.23E-121 ID:SGTC_1712|Compound:st032288|FD-Score:-4.34|P-value:1.66E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-3.27|P-value:8.61E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.13|P-value:3.83E-5 ID:SGTC_2073|Compound:5212005|FD-Score:3.48|P-value:4.80E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.36|P-value:6.50E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.07|P-value:5.48E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-3.81|P-value:1.32E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.76|P-value:2.71E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.95|P-value:1.13E-6 ID:SGTC_2265|Compound:7929201|FD-Score:3.35|P-value:7.40E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-5.10|P-value:5.46E-7 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.25|P-value:2.66E-5 ID:SGTC_2521|Compound:angolensin|FD-Score:3.59|P-value:3.24E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:9.35|P-value:2.96E-19 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.62|P-value:4.50E-8 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:3.70E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:34.10|P-value:5.96E-232 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-4.47|P-value:9.38E-6 ID:SGTC_2683|Compound:piperine|FD-Score:3.50|P-value:4.50E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.51|P-value:8.19E-6 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.00|P-value:6.49E-5 ID:SGTC_2793|Compound:7325131|FD-Score:7.41|P-value:8.97E-13 ID:SGTC_2796|Compound:7949634|FD-Score:-4.12|P-value:4.11E-5 ID:SGTC_2856|Compound:9022925|FD-Score:-4.22|P-value:2.76E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-6.76|P-value:5.39E-11 ID:SGTC_2948|Compound:9070189|FD-Score:3.80|P-value:1.55E-4 ID:SGTC_3000|Compound:9075062|FD-Score:4.16|P-value:3.85E-5 ID:SGTC_3003|Compound:9075612|FD-Score:5.20|P-value:3.94E-7 ID:SGTC_3071|Compound:9117417|FD-Score:-3.90|P-value:9.53E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-8.02|P-value:9.12E-15 ID:SGTC_3130|Compound:9127414|FD-Score:4.50|P-value:9.67E-6 ID:SGTC_3132|Compound:9126706|FD-Score:-3.77|P-value:1.57E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.09|P-value:5.14E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.91|P-value:1.55E-6

Top fitness defect scores for YDR297W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2618 avocadyne 34.10 43.4 µM 5.96E-232 1 2.83E-255
2 SGTC_463 ikarugamyin 29.40 104.0 µM 1.26E-172 1 5.93E-190
3 SGTC_1687 st020778 24.60 55.4 µM 2.23E-121 3 1.59E-133
4 SGTC_457 grayanotoxin iii 12.00 135.0 µM 1.51E-30 5 1.67E-33
5 SGTC_2524 avocatin b 9.35 10.6 µM 2.96E-19 7 4.58E-21
6 SGTC_1495 4469-0029 7.58 237.0 µM 2.74E-13 28 1.70E-14
7 SGTC_2793 7325131 7.41 81.8 µM 8.97E-13 1 6.26E-14
8 SGTC_2581 avocatin a 5.62 64.0 µM 4.50E-8 sphingolipid biosynthesis & PDR1 21 9.35E-9
9 SGTC_3003 9075612 5.20 71.4 µM 3.94E-7 8 1.02E-7
10 SGTC_453 enantio-paf c16 4.95 10.0 µM 1.29E-6 90 3.76E-7
11 SGTC_3211 9128781 4.91 49.5 µM 1.55E-6 40 4.58E-7
12 SGTC_304 1310-0298 4.86 97.0 µM 1.97E-6 cell wall 32 5.97E-7
13 SGTC_505 gw-5074 4.66 63.9 µM 4.78E-6 37 1.58E-6
14 SGTC_8 0354-0001 4.66 70.0 µM 4.79E-6 52 1.59E-6
15 SGTC_3130 9127414 4.50 49.5 µM 9.67E-6 13 3.44E-6
16 SGTC_2502 avocadyne acetate 4.25 18.6 µM 2.66E-5 21 1.05E-5
17 SGTC_1026 k007-1105 4.22 30.6 µM 3.05E-5 ERAD & cell cycle 88 1.22E-5
18 SGTC_1131 3454-3128 4.17 32.3 µM 3.70E-5 35 1.50E-5
19 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.17 70.0 µM 3.70E-5 redox potentiating 66 1.50E-5
20 SGTC_3000 9075062 4.16 71.4 µM 3.85E-5 12 1.57E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.353 4.22E-99 YOR061W CKA2 hom Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
0.246 1.25E-47 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.197 1.45E-30 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.177 4.63E-25 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.169 7.20E-23 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.163 2.43E-21 YLR407W_p YLR407W_p hom Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
0.158 4.25E-20 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.155 2.14E-19 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.146 1.97E-17 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.145 3.91E-17 YJL134W LCB3 hom Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
0.141 1.94E-16 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.139 5.48E-16 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.134 7.69E-15 YKL126W YPK1 hom Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
0.132 1.96E-14 YBL032W HEK2 hom RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
0.128 8.52E-14 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication