YDR309C / GIC2

Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR309C / GIC2

Click on Significant Values for Screen Details ID:SGTC_332|Compound:4112-0506|FD-Score:2.97|P-value:6.41E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.95|P-value:6.73E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.44|P-value:9.38E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.87|P-value:9.24E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-2.98|P-value:5.76E-4 ID:SGTC_917|Compound:harmaline|FD-Score:2.91|P-value:8.02E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.11|P-value:3.61E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:2.98|P-value:6.03E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.48|P-value:7.93E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.48|P-value:7.97E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.02|P-value:4.89E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.50|P-value:5.18E-7 ID:SGTC_1726|Compound:st035556|FD-Score:-4.04|P-value:5.27E-6 ID:SGTC_1731|Compound:st035964|FD-Score:2.90|P-value:8.31E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-6.32|P-value:3.01E-12 ID:SGTC_1735|Compound:st034310|FD-Score:-4.45|P-value:6.07E-7 ID:SGTC_1737|Compound:st035557|FD-Score:-5.69|P-value:2.91E-10 ID:SGTC_1945|Compound:st075991|FD-Score:3.61|P-value:4.34E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.73|P-value:2.40E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.68|P-value:3.18E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.49|P-value:7.67E-5 ID:SGTC_2108|Compound:5365811|FD-Score:3.13|P-value:3.43E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.30|P-value:1.61E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.12|P-value:3.39E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-5.10|P-value:1.35E-8 ID:SGTC_2837|Compound:9008476|FD-Score:2.92|P-value:7.60E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.14|P-value:3.25E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-2.90|P-value:7.96E-4 ID:SGTC_3304|Compound:9125678|FD-Score:9.83|P-value:6.13E-27 ID:SGTC_332|Compound:4112-0506|FD-Score:2.97|P-value:6.41E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:2.95|P-value:6.73E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.44|P-value:9.38E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.87|P-value:9.24E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-2.98|P-value:5.76E-4 ID:SGTC_917|Compound:harmaline|FD-Score:2.91|P-value:8.02E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.11|P-value:3.61E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:2.98|P-value:6.03E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.48|P-value:7.93E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.48|P-value:7.97E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.02|P-value:4.89E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.50|P-value:5.18E-7 ID:SGTC_1726|Compound:st035556|FD-Score:-4.04|P-value:5.27E-6 ID:SGTC_1731|Compound:st035964|FD-Score:2.90|P-value:8.31E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-6.32|P-value:3.01E-12 ID:SGTC_1735|Compound:st034310|FD-Score:-4.45|P-value:6.07E-7 ID:SGTC_1737|Compound:st035557|FD-Score:-5.69|P-value:2.91E-10 ID:SGTC_1945|Compound:st075991|FD-Score:3.61|P-value:4.34E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.73|P-value:2.40E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.68|P-value:3.18E-5 ID:SGTC_2024|Compound:5144227|FD-Score:3.49|P-value:7.67E-5 ID:SGTC_2108|Compound:5365811|FD-Score:3.13|P-value:3.43E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.30|P-value:1.61E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.12|P-value:3.39E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-5.10|P-value:1.35E-8 ID:SGTC_2837|Compound:9008476|FD-Score:2.92|P-value:7.60E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.14|P-value:3.25E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-2.90|P-value:7.96E-4 ID:SGTC_3304|Compound:9125678|FD-Score:9.83|P-value:6.13E-27

Top fitness defect scores for YDR309C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3304 9125678 9.83 46.1 µM 6.13E-27 2 4.37E-23
2 SGTC_1390 3413-2614 4.50 145.0 µM 5.18E-7 fatty acid desaturase (OLE1) 13 3.45E-6
3 SGTC_2021 4019055 3.68 173.0 µM 3.18E-5 endomembrane recycling 139 1.15E-4
4 SGTC_1945 st075991 3.61 56.6 µM 4.34E-5 60S ribosome export 30 1.51E-4
5 SGTC_2024 5144227 3.49 134.0 µM 7.67E-5 54 2.45E-4
6 SGTC_1281 0884-0031 3.48 11.8 µM 7.93E-5 fatty acid desaturase (OLE1) 36 2.52E-4
7 SGTC_1312 1218-2183 3.48 165.0 µM 7.97E-5 15 2.53E-4
8 SGTC_453 enantio-paf c16 3.44 10.0 µM 9.38E-5 189 2.91E-4
9 SGTC_2848 9016370 3.14 26.0 µM 3.25E-4 64 8.43E-4
10 SGTC_2108 5365811 3.13 200.0 µM 3.43E-4 52 8.83E-4
11 SGTC_922 1866-0035 3.11 480.0 µM 3.61E-4 excess fatty acid 114 9.22E-4
12 SGTC_984 1492-0255 2.98 2.9 µM 6.03E-4 SWF1 & branched chain AA biosynthesis 66 0.00143
13 SGTC_332 4112-0506 2.97 40.9 µM 6.41E-4 61 0.00151
14 SGTC_335 4077-0256 2.95 27.6 µM 6.73E-4 52 0.00157
15 SGTC_2837 9008476 2.92 45.5 µM 7.60E-4 87 0.00174
16 SGTC_917 harmaline 2.91 728.1 nM 8.02E-4 ergosterol biosynthesis 80 0.00183
17 SGTC_1731 st035964 2.90 63.0 µM 8.31E-4 88 0.00188
18 SGTC_457 grayanotoxin iii 2.87 135.0 µM 9.24E-4 153 0.00206
19 SGTC_1081 sertraline 2.79 6.0 µM 0.00122 60S ribosome export 81 0.00263
20 SGTC_1230 0170-0365 2.78 3.8 µM 0.00126 endomembrane recycling 180 0.00268

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.345 2.30E-94 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.341 4.56E-92 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.321 5.58E-81 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.259 1.50E-52 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.232 2.62E-42 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.203 1.46E-32 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.189 3.51E-28 YGL049C TIF4632 hom Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
0.171 1.90E-23 YML017W PSP2 hom Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
0.141 2.80E-16 YJR083C ACF4 hom Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
0.140 3.27E-16 YMR068W AVO2 hom Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
0.138 1.16E-15 YJL167W ERG20 het Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
0.136 2.70E-15 YLR189C ATG26 hom UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
0.135 4.47E-15 YGR067C_p YGR067C_p hom Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p
0.133 1.23E-14 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.133 1.36E-14 YGL154C LYS5 hom Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine