HIPHOP chemogenomics database

YDR323C / PEP7

Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR323C / PEP7

Click on Significant Values for Screen Details

Top fitness defect scores for YDR323C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1760	st045453	22.30	10.4 µM	3.32E-61	NEO1-PIK1	6	4.45E-110
2	SGTC_1687	st020778	16.70	55.4 µM	1.76E-35		15	3.92E-63
3	SGTC_365	0121-0052	16.50	13.6 µM	2.04E-34	mitochondrial response to ROS	2	3.39E-61
4	SGTC_748	0136-0228	14.20	77.9 µM	2.91E-26	redox potentiating	2	2.46E-46
5	SGTC_2567	aureobasidin a	13.90	60.0 nM	5.68E-25		6	5.52E-44
6	SGTC_2526	patulin	13.50	37.6 µM	6.74E-24		6	4.98E-42
7	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	12.60	54.9 µM	4.66E-21	redox potentiating	8	7.32E-37
8	SGTC_168	3970-0862	12.30	530.5 µM	5.81E-20		6	7.21E-35
9	SGTC_70	0141-0289	12.00	43.3 µM	3.46E-19	mitochondrial stress	15	1.85E-33
10	SGTC_841	0251-0215	11.70	91.2 µM	2.32E-18		9	5.87E-32
11	SGTC_1803	st050068	11.00	52.4 µM	1.76E-16	NEO1-PIK1	20	1.53E-28
12	SGTC_1789	tunicamycin	10.80	25.0 nM	5.37E-16	unfolded protein response	5	1.16E-27
13	SGTC_2615	purpurogallin-4-carboxylic acid	10.10	100.0 µM	3.13E-14	redox potentiating	8	1.86E-24
14	SGTC_206	4546-0025	10.10	72.0 µM	3.80E-14	NEO1	13	2.65E-24
15	SGTC_1495	4469-0029	10.00	237.0 µM	6.44E-14		17	6.90E-24
16	SGTC_391	gliotoxin	9.85	8.3 µM	1.58E-13		11	3.53E-23
17	SGTC_52	0329-0193	9.68	875.0 nM	4.01E-13	TSC3-RPN4	2	1.90E-22
18	SGTC_1754	st042065	9.40	16.1 µM	1.77E-12	NEO1-PIK1	33	2.80E-21
19	SGTC_670	4204-0025	9.21	119.0 µM	4.75E-12	copper-dependent oxidative stress	3	1.68E-20
20	SGTC_805	3852-0218	8.96	479.0 µM	1.63E-11	ERAD & cell cycle	6	1.56E-19

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.609	0	YOR036W	PEP12	hom	Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.549	1.07E-263	YDR495C	VPS3	hom	Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.496	1.10E-207	YML097C	VPS9	hom	A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.450	1.30E-166	YOR089C	VPS21	hom	Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.399	2.52E-128	YLR417W	VPS36	hom	Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.378	1.41E-114	YAL002W	VPS8	hom	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.374	7.35E-112	YDL077C	VAM6	hom	Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.371	1.11E-109	YGL212W	VAM7	hom	Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.369	7.24E-109	YPR173C	VPS4	hom	AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.368	5.87E-108	YLR025W	SNF7	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.361	7.81E-104	YJR102C	VPS25	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.360	3.19E-103	YGL095C	VPS45	hom	Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.358	5.22E-102	YDR080W	VPS41	hom	Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.350	4.52E-97	YDL192W	ARF1	hom	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.349	1.23E-96	YPL065W	VPS28	hom	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p