YDR333C / RQC1

Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR333C / RQC1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR333C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3303 9125618 8.38 40.6 µM 4.30E-19 5 2.60E-17
2 SGTC_3305 9127027 6.55 53.8 µM 2.32E-12 3 2.95E-11
3 SGTC_3306 9127676 4.79 59.8 µM 2.01E-7 10 8.22E-7
4 SGTC_2483 5729050 4.28 27.2 µM 3.02E-6 18 9.43E-6
5 SGTC_2485 5633444 4.17 17.8 µM 5.17E-6 redox potentiating 35 1.53E-5
6 SGTC_1712 st032288 4.16 53.4 µM 5.28E-6 calcium & mitochondrial duress 176 1.56E-5
7 SGTC_246 cycloheximide 4.01 667.0 µM 1.13E-5 13 3.10E-5
8 SGTC_70 0141-0289 3.93 43.3 µM 1.60E-5 mitochondrial stress 176 4.25E-5
9 SGTC_3299 9121982 3.86 43.1 µM 2.17E-5 34 5.59E-5
10 SGTC_1001 4048-4355 3.85 44.4 µM 2.31E-5 copper-dependent oxidative stress 39 5.91E-5
11 SGTC_212 3807-4682 3.74 38.8 µM 3.76E-5 tubulin folding & SWR complex 79 9.16E-5
12 SGTC_2275 7947463 3.71 200.0 µM 4.25E-5 iron homeostasis 41 1.02E-4
13 SGTC_2354 9060492 3.60 162.0 µM 6.79E-5 26 1.56E-4
14 SGTC_1240 0356-0016 3.59 42.1 µM 7.23E-5 mitochondrial response to ROS 44 1.65E-4
15 SGTC_875 0709-0210 3.43 25.7 µM 1.40E-4 fatty acid desaturase (OLE1) 50 2.99E-4
16 SGTC_1138 3761-0006 3.43 1.0 µM 1.40E-4 12 3.00E-4
17 SGTC_1979 st071809 3.40 45.3 µM 1.62E-4 RPP1 & pyrimidine depletion 29 3.42E-4
18 SGTC_2064 5220758 3.33 200.0 µM 2.14E-4 67 4.40E-4
19 SGTC_2442 5158906 3.21 48.5 µM 3.40E-4 39 6.67E-4
20 SGTC_400 oxethazaine 3.20 37.5 µM 3.55E-4 calcium & mitochondrial duress 41 6.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 4.53E-21 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.141 1.88E-16 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.130 3.40E-14 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.130 3.56E-14 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.117 1.01E-11 YLR191W PEX13 hom Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p
0.114 3.46E-11 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.112 9.40E-11 YGR087C PDC6 hom Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
0.104 1.70E-9 YPL160W CDC60 het Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
0.103 1.92E-9 YCR012W PGK1 het 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
0.103 2.08E-9 YDR219C MFB1 hom Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding
0.101 5.44E-9 YDL136W RPL35B hom Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
0.098 1.23E-8 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.095 3.17E-8 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.095 4.02E-8 YHR001W OSH7 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.094 5.62E-8 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability