YDR375C / BCS1

Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR375C / BCS1

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.46|P-value:5.12E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:3.69|P-value:2.31E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.37|P-value:1.65E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:5.05|P-value:7.64E-7 ID:SGTC_134|Compound:0269-0018|FD-Score:6.19|P-value:1.86E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:6.10|P-value:3.01E-9 ID:SGTC_150|Compound:3970-0969|FD-Score:4.58|P-value:6.77E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.47|P-value:8.05E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:4.52|P-value:8.68E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.59|P-value:3.27E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:30.50|P-value:5.36E-188 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-4.49|P-value:7.28E-6 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.05|P-value:6.01E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:6.66|P-value:1.10E-10 ID:SGTC_386|Compound:1509-0011|FD-Score:8.58|P-value:1.29E-16 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.27|P-value:7.70E-10 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:4.16|P-value:3.91E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.44|P-value:5.50E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.68|P-value:1.87E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.61|P-value:3.05E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.53|P-value:4.07E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.50|P-value:9.47E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.76|P-value:2.98E-6 ID:SGTC_1162|Compound:k048-0130|FD-Score:-4.00|P-value:5.41E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.53|P-value:8.39E-6 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.89|P-value:1.09E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:4.51|P-value:9.03E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.33|P-value:7.84E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:5.37|P-value:1.60E-7 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.20|P-value:9.73E-4 ID:SGTC_1630|Compound:st005209|FD-Score:5.78|P-value:1.88E-8 ID:SGTC_1635|Compound:st007035|FD-Score:22.50|P-value:2.10E-103 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.45|P-value:2.60E-10 ID:SGTC_1927|Compound:st060202|FD-Score:5.45|P-value:1.08E-7 ID:SGTC_2005|Compound:4024620|FD-Score:3.65|P-value:2.67E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.32|P-value:6.42E-4 ID:SGTC_2307|Compound:7538926|FD-Score:5.16|P-value:4.45E-7 ID:SGTC_2314|Compound:7727897|FD-Score:3.63|P-value:2.89E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.13|P-value:4.32E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-3.99|P-value:5.73E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.48|P-value:3.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.01|P-value:5.19E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.41|P-value:1.35E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.10|P-value:3.77E-5 ID:SGTC_3143|Compound:9086658|FD-Score:3.62|P-value:3.01E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.46|P-value:5.12E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:3.69|P-value:2.31E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.37|P-value:1.65E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:5.05|P-value:7.64E-7 ID:SGTC_134|Compound:0269-0018|FD-Score:6.19|P-value:1.86E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:6.10|P-value:3.01E-9 ID:SGTC_150|Compound:3970-0969|FD-Score:4.58|P-value:6.77E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.47|P-value:8.05E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:4.52|P-value:8.68E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.59|P-value:3.27E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:30.50|P-value:5.36E-188 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-4.49|P-value:7.28E-6 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.05|P-value:6.01E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:6.66|P-value:1.10E-10 ID:SGTC_386|Compound:1509-0011|FD-Score:8.58|P-value:1.29E-16 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.27|P-value:7.70E-10 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:4.16|P-value:3.91E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.44|P-value:5.50E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.68|P-value:1.87E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.61|P-value:3.05E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.53|P-value:4.07E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.50|P-value:9.47E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.76|P-value:2.98E-6 ID:SGTC_1162|Compound:k048-0130|FD-Score:-4.00|P-value:5.41E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.53|P-value:8.39E-6 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.89|P-value:1.09E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:4.51|P-value:9.03E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.33|P-value:7.84E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:5.37|P-value:1.60E-7 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.20|P-value:9.73E-4 ID:SGTC_1630|Compound:st005209|FD-Score:5.78|P-value:1.88E-8 ID:SGTC_1635|Compound:st007035|FD-Score:22.50|P-value:2.10E-103 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.45|P-value:2.60E-10 ID:SGTC_1927|Compound:st060202|FD-Score:5.45|P-value:1.08E-7 ID:SGTC_2005|Compound:4024620|FD-Score:3.65|P-value:2.67E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.32|P-value:6.42E-4 ID:SGTC_2307|Compound:7538926|FD-Score:5.16|P-value:4.45E-7 ID:SGTC_2314|Compound:7727897|FD-Score:3.63|P-value:2.89E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.13|P-value:4.32E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-3.99|P-value:5.73E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.48|P-value:3.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.01|P-value:5.19E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.41|P-value:1.35E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.10|P-value:3.77E-5 ID:SGTC_3143|Compound:9086658|FD-Score:3.62|P-value:3.01E-4

Top fitness defect scores for YDR375C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 30.50 5.3 µM 5.36E-188 12 6.16E-205
2 SGTC_1635 st007035 22.50 42.4 µM 2.10E-103 15 1.00E-112
3 SGTC_386 1509-0011 8.58 3.1 µM 1.29E-16 56 4.65E-18
4 SGTC_310 1273-0058 6.66 1.1 µM 1.10E-10 mitochondrial stress 127 1.39E-11
5 SGTC_134 0269-0018 6.19 6.9 µM 1.86E-9 80 3.06E-10
6 SGTC_149 3970-0822 6.10 46.1 µM 3.01E-9 tubulin folding & SWR complex 37 5.18E-10
7 SGTC_1630 st005209 5.78 31.3 µM 1.88E-8 5 3.83E-9
8 SGTC_1927 st060202 5.45 13.8 µM 1.08E-7 6 2.59E-8
9 SGTC_1380 2291-0730 5.37 164.0 µM 1.60E-7 4 3.96E-8
10 SGTC_2307 7538926 5.16 6.3 µM 4.45E-7 16 1.21E-7
11 SGTC_87 0100-0052 5.05 35.5 µM 7.64E-7 50 2.19E-7
12 SGTC_1157 4092-0394 4.76 27.6 µM 2.98E-6 14 9.70E-7
13 SGTC_150 3970-0969 4.58 77.2 µM 6.77E-6 43 2.38E-6
14 SGTC_1182 1222-0449 4.53 220.0 µM 8.39E-6 11 3.00E-6
15 SGTC_168 3970-0862 4.52 530.5 µM 8.68E-6 89 3.12E-6
16 SGTC_1349 flufenamic acid 4.51 69.0 µM 9.03E-6 5 3.25E-6
17 SGTC_1153 4055-0057 4.50 267.0 µM 9.47E-6 12 3.43E-6
18 SGTC_2942 9052777 4.41 62.2 µM 1.35E-5 23 5.06E-6
19 SGTC_59 0330-0160 4.37 1.3 µM 1.65E-5 90 6.30E-6
20 SGTC_494 Boc-Gly-Val-Val-CHO 4.16 140.0 µM 3.91E-5 5 1.62E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.728 0 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.726 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.706 0 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.691 0 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.674 0 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.672 0 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.671 0 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.662 0 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.661 0 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.656 0 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
0.650 0 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.649 0 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.647 0 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.647 0 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.644 0 YER058W PET117 hom Protein required for assembly of cytochrome c oxidase