HIPHOP chemogenomics database

YDR389W / SAC7

GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR389W / SAC7

Click on Significant Values for Screen Details

Top fitness defect scores for YDR389W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_514	4466-0038	12.30	2.6 nM	3.59E-25	cell wall	15	2.88E-35
2	SGTC_2912	7964298	10.70	10.9 µM	1.74E-19	cell wall	12	3.34E-27
3	SGTC_1754	st042065	10.40	16.1 µM	1.57E-18	NEO1-PIK1	21	7.58E-26
4	SGTC_43	0570-0105	9.57	72.5 µM	8.31E-16	ERG2	11	5.52E-22
5	SGTC_733	4069-0007	9.34	56.1 µM	3.75E-15	ERAD & cell cycle	18	4.68E-21
6	SGTC_117	lucigenin	9.15	22.1 µM	1.37E-14	redox potentiating	11	2.95E-20
7	SGTC_70	0141-0289	8.88	43.3 µM	7.45E-14	mitochondrial stress	27	3.26E-19
8	SGTC_15	0266-0086	8.85	27.1 µM	8.89E-14	endomembrane recycling	7	4.19E-19
9	SGTC_916	4466-0038	8.23	42.0 nM	4.01E-12	cell wall	11	9.35E-17
10	SGTC_3037	9091026	7.92	49.5 µM	2.44E-11	NEO1-PIK1	15	1.21E-15
11	SGTC_999	4022-0066	7.50	22.7 µM	2.47E-10	Golgi	8	3.25E-14
12	SGTC_351	caspofungin	7.37	25.0 nM	4.76E-10	cell wall	22	8.22E-14
13	SGTC_9	0092-0002	7.17	10.4 µM	1.41E-9		4	3.85E-13
14	SGTC_2322	7413319	6.92	38.2 µM	4.79E-9	ERAD & cell cycle	51	2.18E-12
15	SGTC_16	erodoxin	6.85	22.8 µM	6.78E-9	unfolded protein response	19	3.58E-12
16	SGTC_1090	efavirenz	6.51	20.7 µM	3.58E-8		1	3.80E-11
17	SGTC_1672	st016598	6.50	6.5 µM	3.68E-8	endomembrane recycling	28	3.95E-11
18	SGTC_2974	9094440	6.50	9.7 µM	3.71E-8	ergosterol biosynthesis	7	4.00E-11
19	SGTC_1874	staurosporine	6.23	7.6 µM	1.27E-7	cell wall signaling	10	2.30E-10
20	SGTC_29	0299-0004	6.21	13.0 µM	1.41E-7		10	2.66E-10

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.270	4.46E-57	YLR330W	CHS5	hom	Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane
0.254	2.00E-50	YBL061C	SKT5	hom	Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.237	3.16E-44	YDR348C	PAL1	hom	Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.236	1.39E-43	YDR162C	NBP2	hom	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.234	5.44E-43	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.223	5.69E-39	YGR166W	TRS65	hom	Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.216	1.04E-36	YNL079C	TPM1	hom	Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
0.216	1.19E-36	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.210	7.10E-35	YKL145W	RPT1	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
0.208	2.73E-34	YLL043W	FPS1	hom	Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.207	6.94E-34	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.200	1.39E-31	YDR351W	SBE2	hom	Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.198	4.92E-31	YJR102C	VPS25	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.198	6.11E-31	YJL081C	ARP4	het	Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.198	7.14E-31	YGL035C	MIG1	hom	Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion