YDR390C / UBA2

Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR390C / UBA2

Click on Significant Values for Screen Details ID:SGTC_5|Compound:0352-0601|FD-Score:3.25|P-value:3.39E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:-3.51|P-value:1.07E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:-2.99|P-value:7.89E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.31|P-value:2.40E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.22|P-value:3.37E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:3.21|P-value:3.97E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.01|P-value:7.56E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:5.12|P-value:4.25E-8 ID:SGTC_761|Compound:k216-0224|FD-Score:6.58|P-value:2.80E-12 ID:SGTC_771|Compound:0250-0039|FD-Score:3.39|P-value:1.94E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.94|P-value:1.87E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.43|P-value:1.49E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.14|P-value:4.58E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-3.88|P-value:2.15E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.20|P-value:4.16E-4 ID:SGTC_2034|Compound:5197015|FD-Score:-2.97|P-value:8.47E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.18|P-value:4.00E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.02|P-value:7.94E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.30|P-value:2.84E-4 ID:SGTC_2355|Compound:9061122|FD-Score:4.34|P-value:2.78E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.41|P-value:1.86E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.12|P-value:5.51E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.06|P-value:6.15E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-4.87|P-value:1.42E-7 ID:SGTC_2517|Compound:totarol|FD-Score:3.15|P-value:5.04E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.56|P-value:9.72E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.01|P-value:8.31E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.25|P-value:3.04E-4 ID:SGTC_2984|Compound:9022109|FD-Score:2.96|P-value:9.94E-4 ID:SGTC_5|Compound:0352-0601|FD-Score:3.25|P-value:3.39E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:-3.51|P-value:1.07E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:-2.99|P-value:7.89E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.31|P-value:2.40E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.22|P-value:3.37E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:3.21|P-value:3.97E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.01|P-value:7.56E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:5.12|P-value:4.25E-8 ID:SGTC_761|Compound:k216-0224|FD-Score:6.58|P-value:2.80E-12 ID:SGTC_771|Compound:0250-0039|FD-Score:3.39|P-value:1.94E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.94|P-value:1.87E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.43|P-value:1.49E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.14|P-value:4.58E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-3.88|P-value:2.15E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.20|P-value:4.16E-4 ID:SGTC_2034|Compound:5197015|FD-Score:-2.97|P-value:8.47E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.18|P-value:4.00E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.02|P-value:7.94E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.30|P-value:2.84E-4 ID:SGTC_2355|Compound:9061122|FD-Score:4.34|P-value:2.78E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.41|P-value:1.86E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.12|P-value:5.51E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.06|P-value:6.15E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-4.87|P-value:1.42E-7 ID:SGTC_2517|Compound:totarol|FD-Score:3.15|P-value:5.04E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.56|P-value:9.72E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.01|P-value:8.31E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.25|P-value:3.04E-4 ID:SGTC_2984|Compound:9022109|FD-Score:2.96|P-value:9.94E-4

Top fitness defect scores for YDR390C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_761 k216-0224 6.58 480.0 µM 2.80E-12 1 2.39E-11 1.92
2 SGTC_505 gw-5074 5.12 63.9 µM 4.25E-8 4 1.55E-7 0.68
3 SGTC_2355 9061122 4.34 103.1 µM 2.78E-6 1 7.04E-6 1.01
4 SGTC_1068 loratadine 3.94 26.9 µM 1.87E-5 6 4.03E-5 0.30
5 SGTC_2540 epiandrosterone 3.56 100.0 µM 9.72E-5 6 1.82E-4 0.05
6 SGTC_2408 sulbentine 3.41 18.4 µM 1.86E-4 48 3.29E-4 0.08
7 SGTC_771 0250-0039 3.39 27.3 µM 1.94E-4 6 3.43E-4 0.13
8 SGTC_2310 7668677 3.30 200.0 µM 2.84E-4 16 4.86E-4 0.01
9 SGTC_5 0352-0601 3.25 109.9 µM 3.39E-4 ERG2 5 5.72E-4 0.07
10 SGTC_281 1672-3440 3.21 14.9 µM 3.97E-4 Golgi 3 6.61E-4 0.06
11 SGTC_1981 st071826 3.20 47.0 µM 4.16E-4 11 6.90E-4 0.01
12 SGTC_2517 totarol 3.15 10.0 µM 5.04E-4 11 8.23E-4 0.08
13 SGTC_2416 tofa 3.12 880.0 nM 5.51E-4 sphingolipid biosynthesis & PDR1 13 8.93E-4 0.22
14 SGTC_1895 st058434 3.06 18.3 µM 7.06E-4 14 0.00112 0.04
15 SGTC_2211 7117706 3.02 200.0 µM 7.94E-4 4 0.00125 0.23
16 SGTC_2846 9008642 3.01 19.5 µM 8.31E-4 16 0.00130 0.05
17 SGTC_2984 9022109 2.96 39.0 µM 9.94E-4 13 0.00153 0.00
18 SGTC_2601 securinine 2.89 100.0 µM 0.00127 38 0.00192 4.05E-4
19 SGTC_279 1273-0055 2.88 2.9 µM 0.00131 azole & statin 8 0.00198 0.01
20 SGTC_319 1348-1596 2.85 45.5 µM 0.00144 RSC & ERG11 17 0.00216 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.44E-16 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.120 3.09E-12 YOR103C OST2 het Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.113 5.43E-11 YDR365C ESF1 het Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
0.107 4.90E-10 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.105 8.86E-10 YGR049W SCM4 hom Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.103 2.33E-9 YKL022C CDC16 het Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
0.099 1.06E-8 YGL001C ERG26 het C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
0.098 1.49E-8 YDL069C CBS1 hom Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
0.098 1.53E-8 YPR058W YMC1 hom Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
0.093 6.93E-8 YGL257C MNT2 hom Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans
0.091 1.29E-7 YGR246C BRF1 het TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
0.091 1.50E-7 YDR325W YCG1 het Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.081 2.43E-6 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.081 2.68E-6 YML005W TRM12 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA
0.081 2.93E-6 YER177W BMH1 hom 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress