YDR397C / NCB2

Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR397C / NCB2

Click on Significant Values for Screen Details ID:SGTC_253|Compound:dyclonine|FD-Score:3.18|P-value:6.64E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.45|P-value:1.73E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:2.73|P-value:5.25E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:2.95|P-value:1.98E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.02|P-value:1.51E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:4.29|P-value:1.27E-7 ID:SGTC_701|Compound:4424-0219|FD-Score:2.58|P-value:9.50E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.59|P-value:7.93E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:4.79|P-value:4.38E-9 ID:SGTC_737|Compound:1950-0117|FD-Score:2.99|P-value:1.64E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:-2.77|P-value:4.48E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:2.72|P-value:5.45E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:3.43|P-value:1.88E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:-3.38|P-value:2.50E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.60|P-value:9.10E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:2.61|P-value:8.41E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.18|P-value:6.72E-5 ID:SGTC_1606|Compound:st000094|FD-Score:3.55|P-value:9.87E-6 ID:SGTC_1612|Compound:st002233|FD-Score:-3.35|P-value:3.02E-5 ID:SGTC_1690|Compound:st023502|FD-Score:3.34|P-value:3.04E-5 ID:SGTC_1691|Compound:st019933|FD-Score:2.58|P-value:9.48E-4 ID:SGTC_1695|Compound:st026914|FD-Score:4.62|P-value:1.48E-8 ID:SGTC_1707|Compound:st032323|FD-Score:-2.92|P-value:2.31E-4 ID:SGTC_1710|Compound:st031758|FD-Score:5.13|P-value:3.71E-10 ID:SGTC_1882|Compound:5615643|FD-Score:2.75|P-value:4.74E-4 ID:SGTC_1900|Compound:st060200|FD-Score:3.20|P-value:6.07E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.26|P-value:4.58E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-4.87|P-value:2.65E-9 ID:SGTC_2029|Compound:5175081|FD-Score:-2.85|P-value:3.15E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-7.47|P-value:1.62E-19 ID:SGTC_2105|Compound:5350118|FD-Score:-3.76|P-value:3.35E-6 ID:SGTC_2116|Compound:5511452|FD-Score:2.73|P-value:5.14E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-2.68|P-value:6.55E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.64|P-value:5.99E-6 ID:SGTC_2137|Compound:5261020|FD-Score:3.00|P-value:1.55E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.46|P-value:1.63E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.04|P-value:1.30E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:2.63|P-value:7.98E-4 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:2.93|P-value:2.12E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:2.59|P-value:9.27E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.63|P-value:6.63E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.33|P-value:3.08E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.87|P-value:1.67E-6 ID:SGTC_2860|Compound:9025725|FD-Score:3.13|P-value:8.56E-5 ID:SGTC_2954|Compound:9074818|FD-Score:2.69|P-value:6.01E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.42|P-value:4.17E-11 ID:SGTC_3017|Compound:9080681|FD-Score:-2.63|P-value:8.17E-4 ID:SGTC_3155|Compound:9099265|FD-Score:2.60|P-value:8.86E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.65|P-value:7.25E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.18|P-value:6.64E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.45|P-value:1.73E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:2.73|P-value:5.25E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:2.95|P-value:1.98E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.02|P-value:1.51E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:4.29|P-value:1.27E-7 ID:SGTC_701|Compound:4424-0219|FD-Score:2.58|P-value:9.50E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.59|P-value:7.93E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:4.79|P-value:4.38E-9 ID:SGTC_737|Compound:1950-0117|FD-Score:2.99|P-value:1.64E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:-2.77|P-value:4.48E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:2.72|P-value:5.45E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:3.43|P-value:1.88E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:-3.38|P-value:2.50E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.60|P-value:9.10E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:2.61|P-value:8.41E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.18|P-value:6.72E-5 ID:SGTC_1606|Compound:st000094|FD-Score:3.55|P-value:9.87E-6 ID:SGTC_1612|Compound:st002233|FD-Score:-3.35|P-value:3.02E-5 ID:SGTC_1690|Compound:st023502|FD-Score:3.34|P-value:3.04E-5 ID:SGTC_1691|Compound:st019933|FD-Score:2.58|P-value:9.48E-4 ID:SGTC_1695|Compound:st026914|FD-Score:4.62|P-value:1.48E-8 ID:SGTC_1707|Compound:st032323|FD-Score:-2.92|P-value:2.31E-4 ID:SGTC_1710|Compound:st031758|FD-Score:5.13|P-value:3.71E-10 ID:SGTC_1882|Compound:5615643|FD-Score:2.75|P-value:4.74E-4 ID:SGTC_1900|Compound:st060200|FD-Score:3.20|P-value:6.07E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.26|P-value:4.58E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-4.87|P-value:2.65E-9 ID:SGTC_2029|Compound:5175081|FD-Score:-2.85|P-value:3.15E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-7.47|P-value:1.62E-19 ID:SGTC_2105|Compound:5350118|FD-Score:-3.76|P-value:3.35E-6 ID:SGTC_2116|Compound:5511452|FD-Score:2.73|P-value:5.14E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-2.68|P-value:6.55E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.64|P-value:5.99E-6 ID:SGTC_2137|Compound:5261020|FD-Score:3.00|P-value:1.55E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.46|P-value:1.63E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.04|P-value:1.30E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:2.63|P-value:7.98E-4 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:2.93|P-value:2.12E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:2.59|P-value:9.27E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.63|P-value:6.63E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.33|P-value:3.08E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.87|P-value:1.67E-6 ID:SGTC_2860|Compound:9025725|FD-Score:3.13|P-value:8.56E-5 ID:SGTC_2954|Compound:9074818|FD-Score:2.69|P-value:6.01E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.42|P-value:4.17E-11 ID:SGTC_3017|Compound:9080681|FD-Score:-2.63|P-value:8.17E-4 ID:SGTC_3155|Compound:9099265|FD-Score:2.60|P-value:8.86E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.65|P-value:7.25E-4

Top fitness defect scores for YDR397C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1710 st031758 5.13 61.5 µM 3.71E-10 1 1.48E-7 1.13
2 SGTC_711 k292-0785 4.79 179.0 µM 4.38E-9 DNA intercalators 4 8.34E-7 0.49
3 SGTC_1695 st026914 4.62 34.5 µM 1.48E-8 2 1.96E-6 0.41
4 SGTC_604 1000-0114 4.29 314.0 µM 1.27E-7 3 8.87E-6 0.82
5 SGTC_2690 usnic acid 3.87 100.0 µM 1.67E-6 6 5.45E-5 0.17
6 SGTC_2125 5246583 3.64 200.0 µM 5.99E-6 tubulin folding & SWR complex 7 1.34E-4 0.02
7 SGTC_2619 obliquin 3.63 87.9 µM 6.63E-6 cell wall 35 1.44E-4 0.15
8 SGTC_702 1488-0190 3.59 94.0 µM 7.93E-6 amide catabolism 2 1.64E-4 0.18
9 SGTC_1606 st000094 3.55 49.2 µM 9.87E-6 60S ribosome export 10 1.91E-4 0.11
10 SGTC_2311 7694451 3.46 200.0 µM 1.63E-5 17 2.73E-4 0.03
11 SGTC_356 1634-0012 3.45 32.5 µM 1.73E-5 10 2.85E-4 0.15
12 SGTC_990 3985-0011 3.43 41.3 µM 1.88E-5 amide catabolism 6 3.01E-4 0.02
13 SGTC_1690 st023502 3.34 41.2 µM 3.04E-5 calcium & mitochondrial duress 6 4.23E-4 0.02
14 SGTC_2633 5,7-dihydroxyisoflavone 3.33 100.0 µM 3.08E-5 10 4.28E-4 0.02
15 SGTC_1900 st060200 3.20 72.8 µM 6.07E-5 8 6.91E-4 0.07
16 SGTC_253 dyclonine 3.18 31.2 µM 6.64E-5 amide catabolism 11 7.37E-4 0.02
17 SGTC_1602 st001878 3.18 87.6 µM 6.72E-5 18 7.43E-4 0.00
18 SGTC_2860 9025725 3.13 39.0 µM 8.56E-5 RPP1 & pyrimidine depletion 10 8.82E-4 0.03
19 SGTC_2437 5741531 3.04 144.2 µM 1.30E-4 20 0.00118 0.01
20 SGTC_2137 5261020 3.00 140.0 µM 1.55E-4 19 0.00134 0.21

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 5.82E-24 YLR438C-A LSM3 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.148 7.84E-18 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.145 3.23E-17 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.132 1.80E-14 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.132 2.06E-14 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.130 3.90E-14 YDR365C ESF1 het Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
0.130 4.65E-14 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
0.129 5.36E-14 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.128 1.04E-13 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.125 3.12E-13 YDR037W KRS1 het Lysyl-tRNA synthetase
0.125 4.12E-13 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.117 1.31E-11 YOL078W AVO1 het Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
0.115 2.35E-11 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.114 4.11E-11 YJL167W ERG20 het Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
0.109 2.45E-10 YDR499W LCD1 het Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress