YDR408C / ADE8

Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR408C / ADE8

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:4.00|P-value:7.53E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:5.62|P-value:5.50E-8 ID:SGTC_87|Compound:0100-0052|FD-Score:3.61|P-value:3.09E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:10.10|P-value:1.52E-21 ID:SGTC_129|Compound:0088-0017|FD-Score:4.27|P-value:2.58E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:-4.47|P-value:1.82E-5 ID:SGTC_264|Compound:promethazine|FD-Score:5.99|P-value:7.81E-9 ID:SGTC_345|Compound:1362-0200|FD-Score:-4.97|P-value:2.16E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:3.32|P-value:7.99E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.85|P-value:2.22E-6 ID:SGTC_698|Compound:k064-0052|FD-Score:3.91|P-value:1.06E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.88|P-value:1.16E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.78|P-value:1.67E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-4.09|P-value:7.94E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:4.15|P-value:4.26E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:-5.35|P-value:3.61E-7 ID:SGTC_1039|Compound:k821-0043|FD-Score:-5.90|P-value:2.31E-8 ID:SGTC_1041|Compound:0206-0046|FD-Score:-4.70|P-value:6.91E-6 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.57|P-value:5.11E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:4.87|P-value:2.07E-6 ID:SGTC_1183|Compound:1254-0324|FD-Score:5.02|P-value:1.03E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.67|P-value:2.47E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.92|P-value:1.51E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.96|P-value:8.59E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.94|P-value:1.39E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.17|P-value:3.95E-5 ID:SGTC_1740|Compound:st038286|FD-Score:5.86|P-value:1.55E-8 ID:SGTC_1744|Compound:st037937|FD-Score:3.50|P-value:4.46E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.58|P-value:4.95E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.45|P-value:5.30E-4 ID:SGTC_1916|Compound:st067606|FD-Score:5.14|P-value:6.02E-7 ID:SGTC_1925|Compound:st057770|FD-Score:6.03|P-value:6.44E-9 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.04|P-value:6.48E-5 ID:SGTC_1957|Compound:st077035|FD-Score:4.46|P-value:1.22E-5 ID:SGTC_2086|Compound:5274770|FD-Score:3.58|P-value:3.34E-4 ID:SGTC_2094|Compound:5332512|FD-Score:-4.41|P-value:2.28E-5 ID:SGTC_2096|Compound:5468907|FD-Score:-6.25|P-value:3.52E-9 ID:SGTC_2097|Compound:5376186|FD-Score:3.58|P-value:3.43E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.30|P-value:2.31E-5 ID:SGTC_2286|Compound:7945697|FD-Score:-4.44|P-value:2.06E-5 ID:SGTC_2472|Compound:5377652|FD-Score:-3.71|P-value:3.18E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.74|P-value:2.82E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-4.31|P-value:3.47E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.74|P-value:1.91E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.36|P-value:6.96E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.74|P-value:2.87E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.82|P-value:1.44E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.35|P-value:7.17E-4 ID:SGTC_2878|Compound:9043973|FD-Score:4.62|P-value:6.07E-6 ID:SGTC_2931|Compound:9014674|FD-Score:-3.65|P-value:3.88E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.47|P-value:4.93E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.36|P-value:9.99E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.98|P-value:8.12E-5 ID:SGTC_3244|Compound:9134489|FD-Score:16.10|P-value:4.04E-52 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.00|P-value:7.53E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:5.62|P-value:5.50E-8 ID:SGTC_87|Compound:0100-0052|FD-Score:3.61|P-value:3.09E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:10.10|P-value:1.52E-21 ID:SGTC_129|Compound:0088-0017|FD-Score:4.27|P-value:2.58E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:-4.47|P-value:1.82E-5 ID:SGTC_264|Compound:promethazine|FD-Score:5.99|P-value:7.81E-9 ID:SGTC_345|Compound:1362-0200|FD-Score:-4.97|P-value:2.16E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:3.32|P-value:7.99E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.85|P-value:2.22E-6 ID:SGTC_698|Compound:k064-0052|FD-Score:3.91|P-value:1.06E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.88|P-value:1.16E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.78|P-value:1.67E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-4.09|P-value:7.94E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:4.15|P-value:4.26E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:-5.35|P-value:3.61E-7 ID:SGTC_1039|Compound:k821-0043|FD-Score:-5.90|P-value:2.31E-8 ID:SGTC_1041|Compound:0206-0046|FD-Score:-4.70|P-value:6.91E-6 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.57|P-value:5.11E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:4.87|P-value:2.07E-6 ID:SGTC_1183|Compound:1254-0324|FD-Score:5.02|P-value:1.03E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.67|P-value:2.47E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.92|P-value:1.51E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.96|P-value:8.59E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.94|P-value:1.39E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.17|P-value:3.95E-5 ID:SGTC_1740|Compound:st038286|FD-Score:5.86|P-value:1.55E-8 ID:SGTC_1744|Compound:st037937|FD-Score:3.50|P-value:4.46E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.58|P-value:4.95E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.45|P-value:5.30E-4 ID:SGTC_1916|Compound:st067606|FD-Score:5.14|P-value:6.02E-7 ID:SGTC_1925|Compound:st057770|FD-Score:6.03|P-value:6.44E-9 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.04|P-value:6.48E-5 ID:SGTC_1957|Compound:st077035|FD-Score:4.46|P-value:1.22E-5 ID:SGTC_2086|Compound:5274770|FD-Score:3.58|P-value:3.34E-4 ID:SGTC_2094|Compound:5332512|FD-Score:-4.41|P-value:2.28E-5 ID:SGTC_2096|Compound:5468907|FD-Score:-6.25|P-value:3.52E-9 ID:SGTC_2097|Compound:5376186|FD-Score:3.58|P-value:3.43E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.30|P-value:2.31E-5 ID:SGTC_2286|Compound:7945697|FD-Score:-4.44|P-value:2.06E-5 ID:SGTC_2472|Compound:5377652|FD-Score:-3.71|P-value:3.18E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.74|P-value:2.82E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-4.31|P-value:3.47E-5 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.74|P-value:1.91E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.36|P-value:6.96E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.74|P-value:2.87E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.82|P-value:1.44E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.35|P-value:7.17E-4 ID:SGTC_2878|Compound:9043973|FD-Score:4.62|P-value:6.07E-6 ID:SGTC_2931|Compound:9014674|FD-Score:-3.65|P-value:3.88E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.47|P-value:4.93E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.36|P-value:9.99E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.98|P-value:8.12E-5 ID:SGTC_3244|Compound:9134489|FD-Score:16.10|P-value:4.04E-52

Top fitness defect scores for YDR408C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3244 9134489 16.10 49.5 µM 4.04E-52 1 6.51E-59
2 SGTC_123 0083-0120 10.10 66.7 µM 1.52E-21 7 4.03E-24
3 SGTC_1925 st057770 6.03 10.1 µM 6.44E-9 TSC3-RPN4 3 8.31E-10
4 SGTC_264 promethazine 5.99 12.5 mM 7.81E-9 DNA intercalators 19 1.03E-9
5 SGTC_1740 st038286 5.86 13.2 µM 1.55E-8 4 2.25E-9
6 SGTC_77 0828-0286 5.62 12.5 µM 5.50E-8 TSC3-RPN4 6 9.36E-9
7 SGTC_1916 st067606 5.14 49.2 µM 6.02E-7 16 1.39E-7
8 SGTC_1183 1254-0324 5.02 198.0 µM 1.03E-6 5 2.56E-7
9 SGTC_1178 0988-0033 4.87 191.0 µM 2.07E-6 15 5.60E-7
10 SGTC_549 1661-1316 4.85 519.0 µM 2.22E-6 6 6.07E-7
11 SGTC_2878 9043973 4.62 26.0 µM 6.07E-6 7 1.88E-6
12 SGTC_1957 st077035 4.46 62.4 µM 1.22E-5 9 4.12E-6
13 SGTC_2099 5377090 4.30 177.4 µM 2.31E-5 15 8.45E-6
14 SGTC_129 0088-0017 4.27 176.6 µM 2.58E-5 63 9.58E-6
15 SGTC_1687 st020778 4.17 55.4 µM 3.95E-5 163 1.54E-5
16 SGTC_962 1254-0300 4.15 21.5 µM 4.26E-5 19 1.68E-5
17 SGTC_1926 methyl fluorone black 4.04 53.6 µM 6.48E-5 DNA intercalators 58 2.69E-5
18 SGTC_46 0329-0187a 4.00 2.2 µM 7.53E-5 73 3.19E-5
19 SGTC_3147 9097027 3.98 49.5 µM 8.12E-5 23 3.46E-5
20 SGTC_1545 lovastatin 3.96 49.4 µM 8.59E-5 24 3.69E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.337 5.06E-90 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.321 4.43E-81 YGR061C ADE6 hom Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.279 7.52E-61 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.277 3.40E-60 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.229 6.67E-41 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.151 1.73E-18 YIL145C PAN6 hom Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
0.119 5.46E-12 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.116 1.48E-11 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.115 2.40E-11 YOL035C_d YOL035C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.112 9.39E-11 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.105 1.06E-9 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.098 1.14E-8 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.093 7.86E-8 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.090 1.92E-7 YGR273C_p YGR273C_p hom Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene
0.089 2.28E-7 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain