HIPHOP chemogenomics database

YDR414C / ERD1

Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR414C / ERD1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR414C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_70	0141-0289	19.40	43.3 µM	1.06E-77	mitochondrial stress	2	4.82E-84
2	SGTC_2567	aureobasidin a	10.30	60.0 nM	2.09E-23		13	2.89E-25
3	SGTC_498	2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)	8.15	150.0 µM	2.87E-15	Golgi	6	1.88E-16
4	SGTC_786	4544-0069	8.01	423.0 µM	7.87E-15		2	5.62E-16
5	SGTC_625	k213-0058	7.65	121.0 µM	1.12E-13	translation	14	9.98E-15
6	SGTC_266	formosanin C	6.69	406.0 nM	7.15E-11	sphingolipid biosynthesis & PDR1	19	1.09E-11
7	SGTC_310	1273-0058	6.50	1.1 µM	2.42E-10	mitochondrial stress	131	4.08E-11
8	SGTC_439	amiodarone	6.44	34.4 µM	3.38E-10	NEO1-PIK1	41	5.86E-11
9	SGTC_255	tunicamycin	6.34	200.5 nM	6.41E-10	unfolded protein response	28	1.17E-10
10	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	6.33	54.9 µM	6.54E-10	redox potentiating	29	1.20E-10
11	SGTC_204	1543-0184	6.12	62.4 µM	2.33E-9	TRP & mitochondrial translation	11	4.76E-10
12	SGTC_453	enantio-paf c16	6.10	10.0 µM	2.65E-9		43	5.46E-10
13	SGTC_2582	agaric acid	5.81	100.0 µM	1.32E-8	redox potentiating	169	3.11E-9
14	SGTC_748	0136-0228	5.80	77.9 µM	1.36E-8	redox potentiating	40	3.22E-9
15	SGTC_1729	st035615	5.68	46.0 µM	2.74E-8		10	6.87E-9
16	SGTC_397	arbidol	5.23	50.0 µM	2.86E-7	cell wall	6	8.70E-8
17	SGTC_2981	9013952	5.20	39.0 µM	3.26E-7		21	1.00E-7
18	SGTC_299	1273-0059	5.20	1.4 µM	3.29E-7		10	1.01E-7
19	SGTC_206	4546-0025	5.03	72.0 µM	7.45E-7	NEO1	41	2.46E-7
20	SGTC_121	0557-0495	4.88	61.3 µM	1.54E-6	calcium & mitochondrial duress	32	5.41E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.301	1.89E-71	YEL042W	GDA1	hom	Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
0.293	2.43E-67	YOR109W	INP53	hom	Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.266	1.34E-55	YNL257C	SIP3	hom	Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.231	4.78E-42	YHR155W	YSP1	hom	Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
0.223	3.87E-39	YDR323C	PEP7	hom	Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.218	2.04E-37	YDR065W	RRG1	hom	Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.203	1.33E-32	YGL008C	PMA1	het	Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.203	2.12E-32	YKL004W	AUR1	het	Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.202	3.81E-32	YKL077W_p	YKL077W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.193	1.54E-29	YDR509W_d	YDR509W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187	8.58E-28	YPL057C	SUR1	hom	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.175	1.64E-24	YGL168W	HUR1	hom	Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
0.173	5.76E-24	YIL022W	TIM44	het	Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.173	6.71E-24	YDL192W	ARF1	hom	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.172	1.20E-23	YJR033C	RAV1	hom	Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate