YDR419W / RAD30

DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR419W / RAD30

Click on Significant Values for Screen Details ID:SGTC_97|Compound:0535-0701|FD-Score:-2.85|P-value:8.60E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:2.82|P-value:7.50E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.93|P-value:4.96E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-3.69|P-value:2.37E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.23|P-value:1.47E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.08|P-value:2.68E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:4.01|P-value:3.49E-6 ID:SGTC_1033|Compound:k072-0102|FD-Score:-2.86|P-value:8.22E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:3.10|P-value:2.46E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.70|P-value:1.64E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:2.81|P-value:7.97E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.94|P-value:1.68E-8 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.82|P-value:9.56E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.64|P-value:3.06E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.08|P-value:3.46E-4 ID:SGTC_1633|Compound:st005664|FD-Score:2.99|P-value:3.95E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.99|P-value:3.93E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-2.83|P-value:9.20E-4 ID:SGTC_1678|Compound:st019265|FD-Score:5.34|P-value:1.22E-9 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.11|P-value:3.06E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.64|P-value:2.22E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-2.84|P-value:8.86E-4 ID:SGTC_1921|Compound:5740136|FD-Score:2.93|P-value:5.02E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.90|P-value:2.15E-8 ID:SGTC_1936|Compound:st074407|FD-Score:5.22|P-value:2.59E-9 ID:SGTC_1974|Compound:st069448|FD-Score:2.94|P-value:4.80E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.84|P-value:7.09E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.83|P-value:7.35E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.28|P-value:1.15E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.19|P-value:1.36E-6 ID:SGTC_2134|Compound:5324836|FD-Score:-3.15|P-value:2.65E-4 ID:SGTC_2167|Compound:5876490|FD-Score:5.84|P-value:3.33E-11 ID:SGTC_2791|Compound:5663188|FD-Score:2.75|P-value:9.90E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.08|P-value:2.73E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.34|P-value:9.04E-5 ID:SGTC_97|Compound:0535-0701|FD-Score:-2.85|P-value:8.60E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:2.82|P-value:7.50E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.93|P-value:4.96E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:-3.69|P-value:2.37E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.23|P-value:1.47E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.08|P-value:2.68E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:4.01|P-value:3.49E-6 ID:SGTC_1033|Compound:k072-0102|FD-Score:-2.86|P-value:8.22E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:3.10|P-value:2.46E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.70|P-value:1.64E-5 ID:SGTC_1193|Compound:1488-0126|FD-Score:2.81|P-value:7.97E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.94|P-value:1.68E-8 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.82|P-value:9.56E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.64|P-value:3.06E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.08|P-value:3.46E-4 ID:SGTC_1633|Compound:st005664|FD-Score:2.99|P-value:3.95E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.99|P-value:3.93E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-2.83|P-value:9.20E-4 ID:SGTC_1678|Compound:st019265|FD-Score:5.34|P-value:1.22E-9 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.11|P-value:3.06E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.64|P-value:2.22E-5 ID:SGTC_1920|Compound:st075728|FD-Score:-2.84|P-value:8.86E-4 ID:SGTC_1921|Compound:5740136|FD-Score:2.93|P-value:5.02E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.90|P-value:2.15E-8 ID:SGTC_1936|Compound:st074407|FD-Score:5.22|P-value:2.59E-9 ID:SGTC_1974|Compound:st069448|FD-Score:2.94|P-value:4.80E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.84|P-value:7.09E-4 ID:SGTC_2057|Compound:5236571|FD-Score:2.83|P-value:7.35E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.28|P-value:1.15E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.19|P-value:1.36E-6 ID:SGTC_2134|Compound:5324836|FD-Score:-3.15|P-value:2.65E-4 ID:SGTC_2167|Compound:5876490|FD-Score:5.84|P-value:3.33E-11 ID:SGTC_2791|Compound:5663188|FD-Score:2.75|P-value:9.90E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.08|P-value:2.73E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.34|P-value:9.04E-5

Top fitness defect scores for YDR419W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2167 5876490 5.84 200.0 µM 3.33E-11 5 2.60E-9
2 SGTC_1678 st019265 5.34 80.2 µM 1.22E-9 60S ribosome export 5 4.77E-8
3 SGTC_1936 st074407 5.22 74.3 µM 2.59E-9 DNA damage response 9 8.80E-8
4 SGTC_1203 3992-3522 4.94 103.0 µM 1.68E-8 12 4.00E-7
5 SGTC_1923 st058458 4.90 19.1 µM 2.15E-8 16 4.88E-7
6 SGTC_2127 5253358 4.19 187.9 µM 1.36E-6 20 1.41E-5
7 SGTC_997 3996-0078 4.01 137.0 µM 3.49E-6 20 3.02E-5
8 SGTC_1094 duloxetine 3.70 25.4 µM 1.64E-5 RPP1 & pyrimidine depletion 19 1.06E-4
9 SGTC_1894 3',6'-dihydroxyflavone 3.64 78.7 µM 2.22E-5 17 1.35E-4
10 SGTC_3033 9090887 3.34 49.5 µM 9.04E-5 fatty acid desaturase (OLE1) 52 4.24E-4
11 SGTC_2126 5251174 3.28 33.9 µM 1.15E-4 64 5.14E-4
12 SGTC_911 3456-4043 3.23 236.0 µM 1.47E-4 mitochondrial processes 57 6.29E-4
13 SGTC_1089 terbinafine 3.10 2.2 µM 2.46E-4 44 9.58E-4
14 SGTC_913 3487-1245 3.08 167.0 µM 2.68E-4 78 0.00103
15 SGTC_2933 9026794 3.08 32.5 µM 2.73E-4 70 0.00104
16 SGTC_1639 Lupinine o-methylbenzoicacid ester hydrochloride 2.99 61.8 µM 3.93E-4 ergosterol biosynthesis 45 0.00140
17 SGTC_1633 st005664 2.99 67.9 µM 3.95E-4 87 0.00141
18 SGTC_1974 st069448 2.94 26.9 µM 4.80E-4 59 0.00165
19 SGTC_310 1273-0058 2.93 1.1 µM 4.96E-4 mitochondrial stress 361 0.00169
20 SGTC_1921 5740136 2.93 50.0 µM 5.02E-4 TRP & mitochondrial translation 128 0.00171

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 8.30E-18 YPR196W_p YPR196W_p hom Putative maltose-responsive transcription factor
0.143 9.82E-17 YHR049W FSH1 hom Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
0.140 4.66E-16 YKL054C DEF1 hom RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
0.130 4.52E-14 YIL120W QDR1 hom Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication
0.128 1.13E-13 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.124 4.63E-13 YDR308C SRB7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.115 2.63E-11 YPL079W RPL21B hom Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
0.115 2.70E-11 YER106W MAM1 hom Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
0.114 3.71E-11 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.112 8.71E-11 YMR085W_p YMR085W_p hom Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
0.110 1.54E-10 YGL153W PEX14 hom Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
0.105 1.03E-9 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.104 1.64E-9 YHR188C GPI16 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
0.104 1.78E-9 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.101 5.24E-9 YJL160C_p YJL160C_p hom Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication