YDR420W / HKR1

Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR420W / HKR1

Click on Significant Values for Screen Details ID:SGTC_245|Compound:methotrexate|FD-Score:2.85|P-value:5.56E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:7.03|P-value:2.63E-16 ID:SGTC_360|Compound:0957-0399|FD-Score:5.25|P-value:7.69E-10 ID:SGTC_369|Compound:0957-0393|FD-Score:3.99|P-value:2.34E-6 ID:SGTC_443|Compound:clozapine|FD-Score:2.81|P-value:6.64E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.41|P-value:1.98E-7 ID:SGTC_497|Compound:capsazepine|FD-Score:3.28|P-value:8.66E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.14|P-value:1.58E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.35|P-value:6.18E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:2.85|P-value:5.46E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.56|P-value:2.26E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.86|P-value:3.95E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.23|P-value:1.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:2.84|P-value:5.90E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:-2.99|P-value:2.23E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:2.73|P-value:9.13E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.38|P-value:5.20E-5 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.29|P-value:8.13E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.43|P-value:2.86E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:2.75|P-value:8.37E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.68|P-value:8.21E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:2.88|P-value:4.94E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:2.92|P-value:4.21E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.19|P-value:8.91E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.69|P-value:7.79E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.04|P-value:2.50E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-2.76|P-value:5.99E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-2.93|P-value:2.85E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.56|P-value:1.50E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.15|P-value:1.10E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.44|P-value:1.71E-7 ID:SGTC_1717|Compound:st033143|FD-Score:3.20|P-value:1.22E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-3.07|P-value:1.56E-4 ID:SGTC_1748|Compound:st037455|FD-Score:2.91|P-value:4.30E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-2.93|P-value:2.91E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-2.69|P-value:7.84E-4 ID:SGTC_2155|Compound:5570423|FD-Score:2.93|P-value:3.95E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:4.15|P-value:9.53E-7 ID:SGTC_2303|Compound:7774051|FD-Score:3.90|P-value:3.86E-6 ID:SGTC_2451|Compound:5856223|FD-Score:-4.43|P-value:1.08E-7 ID:SGTC_2575|Compound:plumbagin|FD-Score:-2.71|P-value:7.23E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.37|P-value:3.91E-5 ID:SGTC_2704|Compound:st078022|FD-Score:2.82|P-value:6.38E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-2.78|P-value:5.43E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.70|P-value:7.48E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.63|P-value:1.07E-5 ID:SGTC_2833|Compound:9001315|FD-Score:-2.86|P-value:3.92E-4 ID:SGTC_2924|Compound:9010168|FD-Score:-2.78|P-value:5.34E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-2.76|P-value:5.84E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.67|P-value:8.38E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.31|P-value:4.77E-10 ID:SGTC_245|Compound:methotrexate|FD-Score:2.85|P-value:5.56E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:7.03|P-value:2.63E-16 ID:SGTC_360|Compound:0957-0399|FD-Score:5.25|P-value:7.69E-10 ID:SGTC_369|Compound:0957-0393|FD-Score:3.99|P-value:2.34E-6 ID:SGTC_443|Compound:clozapine|FD-Score:2.81|P-value:6.64E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.41|P-value:1.98E-7 ID:SGTC_497|Compound:capsazepine|FD-Score:3.28|P-value:8.66E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.14|P-value:1.58E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.35|P-value:6.18E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:2.85|P-value:5.46E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.56|P-value:2.26E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.86|P-value:3.95E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.23|P-value:1.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:2.84|P-value:5.90E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:-2.99|P-value:2.23E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:2.73|P-value:9.13E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.38|P-value:5.20E-5 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.29|P-value:8.13E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.43|P-value:2.86E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:2.75|P-value:8.37E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.68|P-value:8.21E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:2.88|P-value:4.94E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:2.92|P-value:4.21E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.19|P-value:8.91E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.69|P-value:7.79E-6 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.04|P-value:2.50E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-2.76|P-value:5.99E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-2.93|P-value:2.85E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-3.56|P-value:1.50E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.15|P-value:1.10E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.44|P-value:1.71E-7 ID:SGTC_1717|Compound:st033143|FD-Score:3.20|P-value:1.22E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-3.07|P-value:1.56E-4 ID:SGTC_1748|Compound:st037455|FD-Score:2.91|P-value:4.30E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-2.93|P-value:2.91E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-2.69|P-value:7.84E-4 ID:SGTC_2155|Compound:5570423|FD-Score:2.93|P-value:3.95E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:4.15|P-value:9.53E-7 ID:SGTC_2303|Compound:7774051|FD-Score:3.90|P-value:3.86E-6 ID:SGTC_2451|Compound:5856223|FD-Score:-4.43|P-value:1.08E-7 ID:SGTC_2575|Compound:plumbagin|FD-Score:-2.71|P-value:7.23E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.37|P-value:3.91E-5 ID:SGTC_2704|Compound:st078022|FD-Score:2.82|P-value:6.38E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-2.78|P-value:5.43E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.70|P-value:7.48E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.63|P-value:1.07E-5 ID:SGTC_2833|Compound:9001315|FD-Score:-2.86|P-value:3.92E-4 ID:SGTC_2924|Compound:9010168|FD-Score:-2.78|P-value:5.34E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-2.76|P-value:5.84E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.67|P-value:8.38E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.31|P-value:4.77E-10

Top fitness defect scores for YDR420W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_358 0075-0058 7.03 9.4 µM 2.63E-16 9 1.05E-12
2 SGTC_3209 9113616 5.31 49.5 µM 4.77E-10 4 5.39E-8
3 SGTC_360 0957-0399 5.25 1.0 µM 7.69E-10 cell wall 20 7.72E-8
4 SGTC_1713 st033146 4.44 12.8 µM 1.71E-7 17 4.60E-6
5 SGTC_457 grayanotoxin iii 4.41 135.0 µM 1.98E-7 47 5.14E-6
6 SGTC_2251 lansoprazole 4.15 556.0 µM 9.53E-7 18 1.69E-5
7 SGTC_369 0957-0393 3.99 4.5 µM 2.34E-6 cell wall signaling 23 3.35E-5
8 SGTC_2303 7774051 3.90 200.0 µM 3.86E-6 20 4.91E-5
9 SGTC_632 0781-2353 3.56 4.7 µM 2.26E-5 Golgi 58 1.88E-4
10 SGTC_987 1497-1013 3.38 404.0 µM 5.20E-5 23 3.56E-4
11 SGTC_620 0109-0052 3.35 432.0 µM 6.18E-5 38 4.06E-4
12 SGTC_1041 0206-0046 3.29 107.0 µM 8.13E-5 54 5.01E-4
13 SGTC_497 capsazepine 3.28 133.0 µM 8.66E-5 33 5.26E-4
14 SGTC_820 1582-0060 3.23 59.9 µM 1.06E-4 68 6.12E-4
15 SGTC_1717 st033143 3.20 58.0 µM 1.22E-4 82 6.82E-4
16 SGTC_611 3453-1652 3.14 23.7 µM 1.58E-4 60 8.32E-4
17 SGTC_1569 carminic acid 3.04 40.6 µM 2.50E-4 51 0.00118
18 SGTC_2155 5570423 2.93 76.0 µM 3.95E-4 ergosterol biosynthesis 46 0.00168
19 SGTC_1497 4476-2978 2.92 41.3 µM 4.21E-4 79 0.00177
20 SGTC_1748 st037455 2.91 53.6 µM 4.30E-4 92 0.00179

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.191 7.09E-29 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.161 5.24E-21 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.160 1.47E-20 YHL007C STE20 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
0.159 2.18E-20 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.150 2.24E-18 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.143 1.00E-16 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
0.131 2.51E-14 YOR047C STD1 hom Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]
0.127 1.78E-13 YGL210W YPT32 hom Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
0.123 7.16E-13 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.120 2.94E-12 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.116 1.37E-11 YOL071W EMI5 hom Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
0.114 3.93E-11 YBR144C_d YBR144C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
0.113 4.99E-11 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.111 1.04E-10 YDL230W PTP1 hom Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
0.107 4.72E-10 YOR263C_d YOR263C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W