Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YDR443C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.344 | 9.92E-94 | YPL042C | SSN3 | hom | Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression |
0.247 | 6.58E-48 | YDR442W_d | YDR442W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.221 | 2.51E-38 | YOR174W | MED4 | het | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
0.195 | 4.94E-30 | YDR466W | PKH3 | hom | Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant |
0.191 | 6.27E-29 | YHR205W | SCH9 | hom | AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan |
0.187 | 1.12E-27 | YNL025C | SSN8 | hom | Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance |
0.184 | 4.43E-27 | YDL019C | OSH2 | hom | Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability |
0.175 | 2.25E-24 | YDR467C_d | YDR467C_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.168 | 1.40E-22 | YPR070W | MED1 | hom | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
0.167 | 2.62E-22 | YEL022W | GEA2 | hom | Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication |
0.164 | 1.54E-21 | YDR156W | RPA14 | hom | RNA polymerase I subunit A14 |
0.162 | 2.96E-21 | YGL035C | MIG1 | hom | Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion |
0.162 | 3.30E-21 | YOR312C | RPL20B | hom | Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication |
0.157 | 5.13E-20 | YDL136W | RPL35B | hom | Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication |
0.157 | 4.98E-20 | YDL119C_p | YDL119C_p | hom | Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria |