HIPHOP chemogenomics database

YDR443C / SSN2

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR443C / SSN2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR443C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_227	fluconazole	9.13	33.5 µM	3.66E-15	azole & statin	18	3.53E-20
2	SGTC_2688	kanamycin b	8.58	96.7 µM	1.26E-13	calcium & mitochondrial duress	49	4.70E-18
3	SGTC_1712	st032288	8.03	53.4 µM	3.61E-12	calcium & mitochondrial duress	46	4.88E-16
4	SGTC_1877	wortmannin	7.44	25.0 µM	1.01E-10		27	4.85E-14
5	SGTC_1959	st077091	7.29	55.8 µM	2.31E-10	RPP1 & pyrimidine depletion	2	1.52E-13
6	SGTC_682	0kpi-0099	6.31	17.0 µM	3.28E-8		22	1.39E-10
7	SGTC_214	4373-3496	5.89	70.0 µM	2.27E-7		13	1.97E-9
8	SGTC_3308	9128332	5.61	17.6 µM	7.39E-7	plasma membrane duress	42	9.92E-9
9	SGTC_547	1120-0019	5.25	610.0 nM	3.27E-6	azole & statin	12	7.57E-8
10	SGTC_2948	9070189	5.06	9.4 µM	6.77E-6	plasma membrane duress	30	2.04E-7
11	SGTC_1397	3532-0190	4.98	20.1 µM	9.50E-6		2	3.24E-7
12	SGTC_1750	st081859	4.89	2.4 µM	1.33E-5	endomembrane recycling	141	5.11E-7
13	SGTC_212	3807-4682	4.87	38.8 µM	1.39E-5	tubulin folding & SWR complex	39	5.45E-7
14	SGTC_308	4092-1169	4.85	104.0 µM	1.52E-5	plasma membrane duress	31	6.13E-7
15	SGTC_1027	k015-0025	4.51	8.7 µM	5.21E-5		17	3.28E-6
16	SGTC_791	1348-1292	4.50	225.0 µM	5.31E-5		23	3.37E-6
17	SGTC_861	0443-0273	4.39	110.0 µM	7.73E-5		6	5.61E-6
18	SGTC_1022	4477-0945	4.32	21.3 µM	9.94E-5		14	7.89E-6
19	SGTC_1132	3456-3614	4.30	2.5 µM	1.06E-4	tubulin folding & SWR complex	28	8.60E-6
20	SGTC_625	k213-0058	4.25	121.0 µM	1.23E-4	translation	85	1.05E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.344	9.92E-94	YPL042C	SSN3	hom	Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
0.247	6.58E-48	YDR442W_d	YDR442W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.221	2.51E-38	YOR174W	MED4	het	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.195	4.94E-30	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.191	6.27E-29	YHR205W	SCH9	hom	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.187	1.12E-27	YNL025C	SSN8	hom	Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
0.184	4.43E-27	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.175	2.25E-24	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168	1.40E-22	YPR070W	MED1	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.167	2.62E-22	YEL022W	GEA2	hom	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.164	1.54E-21	YDR156W	RPA14	hom	RNA polymerase I subunit A14
0.162	2.96E-21	YGL035C	MIG1	hom	Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.162	3.30E-21	YOR312C	RPL20B	hom	Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.157	5.13E-20	YDL136W	RPL35B	hom	Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
0.157	4.98E-20	YDL119C_p	YDL119C_p	hom	Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria