YDR443C / SSN2

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR443C / SSN2

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-3.90|P-value:6.56E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.91|P-value:4.28E-9 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.27|P-value:2.11E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.16|P-value:3.01E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.06|P-value:2.29E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.87|P-value:1.39E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:5.89|P-value:2.27E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.72|P-value:6.51E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:9.13|P-value:3.66E-15 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.65|P-value:1.38E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:4.85|P-value:1.52E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:-4.41|P-value:1.35E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:4.12|P-value:1.92E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-4.47|P-value:1.09E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.19|P-value:2.73E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:5.25|P-value:3.27E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:4.25|P-value:1.23E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.65|P-value:7.97E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:3.81|P-value:5.04E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.98|P-value:2.95E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.91|P-value:3.61E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:6.31|P-value:3.28E-8 ID:SGTC_789|Compound:1309-1135|FD-Score:3.59|P-value:9.53E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:4.50|P-value:5.31E-5 ID:SGTC_861|Compound:0443-0273|FD-Score:4.39|P-value:7.73E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:4.32|P-value:9.94E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.51|P-value:5.21E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.05|P-value:4.20E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.30|P-value:1.06E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.25|P-value:6.69E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.73|P-value:6.27E-4 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.98|P-value:9.50E-6 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.17|P-value:1.63E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.04|P-value:2.43E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.70|P-value:6.85E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.60|P-value:9.30E-4 ID:SGTC_1666|Compound:st014829|FD-Score:3.69|P-value:7.00E-4 ID:SGTC_1700|Compound:st030840|FD-Score:4.18|P-value:1.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:8.03|P-value:3.61E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.30|P-value:1.90E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-7.32|P-value:5.18E-10 ID:SGTC_1750|Compound:st081859|FD-Score:4.89|P-value:1.33E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:7.44|P-value:1.01E-10 ID:SGTC_1959|Compound:st077091|FD-Score:7.29|P-value:2.31E-10 ID:SGTC_1970|Compound:st070122|FD-Score:4.00|P-value:2.80E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-4.06|P-value:4.00E-4 ID:SGTC_2330|Compound:7979373|FD-Score:4.07|P-value:2.20E-4 ID:SGTC_2342|Compound:7990637|FD-Score:-4.01|P-value:4.72E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-4.39|P-value:1.44E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.68|P-value:7.31E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-7.70|P-value:6.64E-11 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.81|P-value:3.39E-5 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-4.13|P-value:3.26E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-4.59|P-value:7.42E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.07|P-value:2.19E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.58|P-value:1.26E-13 ID:SGTC_2696|Compound:st077691|FD-Score:4.11|P-value:1.95E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-6.55|P-value:2.49E-8 ID:SGTC_2896|Compound:9052656|FD-Score:4.04|P-value:2.48E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.71|P-value:6.62E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.06|P-value:6.77E-6 ID:SGTC_3030|Compound:9087619|FD-Score:3.90|P-value:3.74E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.09|P-value:2.07E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.87|P-value:2.72E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-4.75|P-value:4.17E-5 ID:SGTC_3308|Compound:9128332|FD-Score:5.61|P-value:7.39E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.90|P-value:6.56E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.91|P-value:4.28E-9 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.27|P-value:2.11E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.16|P-value:3.01E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.06|P-value:2.29E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.87|P-value:1.39E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:5.89|P-value:2.27E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.72|P-value:6.51E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:9.13|P-value:3.66E-15 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.65|P-value:1.38E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:4.85|P-value:1.52E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:-4.41|P-value:1.35E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:4.12|P-value:1.92E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-4.47|P-value:1.09E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.19|P-value:2.73E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:5.25|P-value:3.27E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:4.25|P-value:1.23E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.65|P-value:7.97E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:3.81|P-value:5.04E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.98|P-value:2.95E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.91|P-value:3.61E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:6.31|P-value:3.28E-8 ID:SGTC_789|Compound:1309-1135|FD-Score:3.59|P-value:9.53E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:4.50|P-value:5.31E-5 ID:SGTC_861|Compound:0443-0273|FD-Score:4.39|P-value:7.73E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:4.32|P-value:9.94E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.51|P-value:5.21E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.05|P-value:4.20E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.30|P-value:1.06E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-5.25|P-value:6.69E-6 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.73|P-value:6.27E-4 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.98|P-value:9.50E-6 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.17|P-value:1.63E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.04|P-value:2.43E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.70|P-value:6.85E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.60|P-value:9.30E-4 ID:SGTC_1666|Compound:st014829|FD-Score:3.69|P-value:7.00E-4 ID:SGTC_1700|Compound:st030840|FD-Score:4.18|P-value:1.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:8.03|P-value:3.61E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.30|P-value:1.90E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-7.32|P-value:5.18E-10 ID:SGTC_1750|Compound:st081859|FD-Score:4.89|P-value:1.33E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:7.44|P-value:1.01E-10 ID:SGTC_1959|Compound:st077091|FD-Score:7.29|P-value:2.31E-10 ID:SGTC_1970|Compound:st070122|FD-Score:4.00|P-value:2.80E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-4.06|P-value:4.00E-4 ID:SGTC_2330|Compound:7979373|FD-Score:4.07|P-value:2.20E-4 ID:SGTC_2342|Compound:7990637|FD-Score:-4.01|P-value:4.72E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-4.39|P-value:1.44E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.68|P-value:7.31E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-7.70|P-value:6.64E-11 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.81|P-value:3.39E-5 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-4.13|P-value:3.26E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-4.59|P-value:7.42E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.07|P-value:2.19E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.58|P-value:1.26E-13 ID:SGTC_2696|Compound:st077691|FD-Score:4.11|P-value:1.95E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-6.55|P-value:2.49E-8 ID:SGTC_2896|Compound:9052656|FD-Score:4.04|P-value:2.48E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.71|P-value:6.62E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.06|P-value:6.77E-6 ID:SGTC_3030|Compound:9087619|FD-Score:3.90|P-value:3.74E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.09|P-value:2.07E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-4.87|P-value:2.72E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-4.75|P-value:4.17E-5 ID:SGTC_3308|Compound:9128332|FD-Score:5.61|P-value:7.39E-7

Top fitness defect scores for YDR443C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_227 fluconazole 9.13 33.5 µM 3.66E-15 azole & statin 18 3.53E-20
2 SGTC_2688 kanamycin b 8.58 96.7 µM 1.26E-13 calcium & mitochondrial duress 49 4.70E-18
3 SGTC_1712 st032288 8.03 53.4 µM 3.61E-12 calcium & mitochondrial duress 46 4.88E-16
4 SGTC_1877 wortmannin 7.44 25.0 µM 1.01E-10 27 4.85E-14
5 SGTC_1959 st077091 7.29 55.8 µM 2.31E-10 RPP1 & pyrimidine depletion 2 1.52E-13
6 SGTC_682 0kpi-0099 6.31 17.0 µM 3.28E-8 22 1.39E-10
7 SGTC_214 4373-3496 5.89 70.0 µM 2.27E-7 13 1.97E-9
8 SGTC_3308 9128332 5.61 17.6 µM 7.39E-7 plasma membrane duress 42 9.92E-9
9 SGTC_547 1120-0019 5.25 610.0 nM 3.27E-6 azole & statin 12 7.57E-8
10 SGTC_2948 9070189 5.06 9.4 µM 6.77E-6 plasma membrane duress 30 2.04E-7
11 SGTC_1397 3532-0190 4.98 20.1 µM 9.50E-6 2 3.24E-7
12 SGTC_1750 st081859 4.89 2.4 µM 1.33E-5 endomembrane recycling 141 5.11E-7
13 SGTC_212 3807-4682 4.87 38.8 µM 1.39E-5 tubulin folding & SWR complex 39 5.45E-7
14 SGTC_308 4092-1169 4.85 104.0 µM 1.52E-5 plasma membrane duress 31 6.13E-7
15 SGTC_1027 k015-0025 4.51 8.7 µM 5.21E-5 17 3.28E-6
16 SGTC_791 1348-1292 4.50 225.0 µM 5.31E-5 23 3.37E-6
17 SGTC_861 0443-0273 4.39 110.0 µM 7.73E-5 6 5.61E-6
18 SGTC_1022 4477-0945 4.32 21.3 µM 9.94E-5 14 7.89E-6
19 SGTC_1132 3456-3614 4.30 2.5 µM 1.06E-4 tubulin folding & SWR complex 28 8.60E-6
20 SGTC_625 k213-0058 4.25 121.0 µM 1.23E-4 translation 85 1.05E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.344 9.92E-94 YPL042C SSN3 hom Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
0.247 6.58E-48 YDR442W_d YDR442W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.221 2.51E-38 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.195 4.94E-30 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.191 6.27E-29 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.187 1.12E-27 YNL025C SSN8 hom Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
0.184 4.43E-27 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.175 2.25E-24 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168 1.40E-22 YPR070W MED1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.167 2.62E-22 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.164 1.54E-21 YDR156W RPA14 hom RNA polymerase I subunit A14
0.162 2.96E-21 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.162 3.30E-21 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.157 5.13E-20 YDL136W RPL35B hom Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
0.157 4.98E-20 YDL119C_p YDL119C_p hom Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria