YDR445C_d

Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR445C / YDR445C

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.30|P-value:7.73E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.52|P-value:3.69E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.53|P-value:4.67E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.25|P-value:3.33E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:3.64|P-value:3.15E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:4.14|P-value:4.97E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:3.47|P-value:5.66E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:5.19|P-value:5.12E-7 ID:SGTC_472|Compound:calcifediol|FD-Score:3.70|P-value:2.64E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.48|P-value:5.60E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.31|P-value:9.78E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.48|P-value:1.28E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.33|P-value:2.65E-7 ID:SGTC_1456|Compound:k015-0012|FD-Score:-4.69|P-value:3.63E-6 ID:SGTC_1608|Compound:st001473|FD-Score:3.35|P-value:8.39E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.69|P-value:2.72E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.36|P-value:8.27E-4 ID:SGTC_1819|Compound:st053448|FD-Score:3.33|P-value:9.07E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.44|P-value:6.41E-4 ID:SGTC_2058|Compound:5236698|FD-Score:3.34|P-value:8.88E-4 ID:SGTC_2245|Compound:6637752|FD-Score:6.21|P-value:2.48E-9 ID:SGTC_2251|Compound:lansoprazole|FD-Score:7.29|P-value:3.10E-12 ID:SGTC_2262|Compound:9014885|FD-Score:-8.14|P-value:4.57E-15 ID:SGTC_2518|Compound:prenyletin|FD-Score:-3.42|P-value:5.29E-4 ID:SGTC_2986|Compound:9022445|FD-Score:-4.59|P-value:5.79E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.30|P-value:7.73E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.52|P-value:3.69E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.53|P-value:4.67E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.25|P-value:3.33E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:3.64|P-value:3.15E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:4.14|P-value:4.97E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:3.47|P-value:5.66E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:5.19|P-value:5.12E-7 ID:SGTC_472|Compound:calcifediol|FD-Score:3.70|P-value:2.64E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.48|P-value:5.60E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.31|P-value:9.78E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.48|P-value:1.28E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.33|P-value:2.65E-7 ID:SGTC_1456|Compound:k015-0012|FD-Score:-4.69|P-value:3.63E-6 ID:SGTC_1608|Compound:st001473|FD-Score:3.35|P-value:8.39E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.69|P-value:2.72E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.36|P-value:8.27E-4 ID:SGTC_1819|Compound:st053448|FD-Score:3.33|P-value:9.07E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.44|P-value:6.41E-4 ID:SGTC_2058|Compound:5236698|FD-Score:3.34|P-value:8.88E-4 ID:SGTC_2245|Compound:6637752|FD-Score:6.21|P-value:2.48E-9 ID:SGTC_2251|Compound:lansoprazole|FD-Score:7.29|P-value:3.10E-12 ID:SGTC_2262|Compound:9014885|FD-Score:-8.14|P-value:4.57E-15 ID:SGTC_2518|Compound:prenyletin|FD-Score:-3.42|P-value:5.29E-4 ID:SGTC_2986|Compound:9022445|FD-Score:-4.59|P-value:5.79E-6

Top fitness defect scores for YDR445C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2251 lansoprazole 7.29 556.0 µM 3.10E-12 2 1.54E-13
2 SGTC_2245 6637752 6.21 200.0 µM 2.48E-9 7 2.69E-10
3 SGTC_1454 4239-0054 5.33 42.6 µM 2.65E-7 7 4.95E-8
4 SGTC_392 N-ethylmaleimide 5.19 8.3 µM 5.12E-7 RNA pol III & RNase P/MRP 2 1.03E-7
5 SGTC_1447 4239-0816 4.48 81.9 µM 1.28E-5 19 3.76E-6
6 SGTC_135 0933-0004 4.25 26.4 µM 3.33E-5 60S ribosome export 15 1.09E-5
7 SGTC_240 5hr heat shock (37°C) + Streptovitacin 4.14 300.0 µM 4.97E-5 heat shock/prefoldin 31 1.70E-5
8 SGTC_472 calcifediol 3.70 10.0 µM 2.64E-4 9 1.10E-4
9 SGTC_1629 st005085 3.69 44.9 µM 2.72E-4 RPP1 & pyrimidine depletion 46 1.14E-4
10 SGTC_136 0097-0015 3.64 404.5 µM 3.15E-4 60S ribosome export 28 1.34E-4
11 SGTC_127 0180-0333 3.53 59.1 µM 4.67E-4 mitochondrial response to ROS 64 2.08E-4
12 SGTC_923 1900-2434 3.48 73.3 µM 5.60E-4 RNA processing & uracil transport 42 2.55E-4
13 SGTC_365 0121-0052 3.47 13.6 µM 5.66E-4 mitochondrial response to ROS 82 2.58E-4
14 SGTC_1951 sophoraflavanone G 3.44 9.2 µM 6.41E-4 RNA processing & uracil transport 52 2.96E-4
15 SGTC_1808 st050063 3.36 61.5 µM 8.27E-4 30 3.94E-4
16 SGTC_1608 st001473 3.35 40.5 µM 8.39E-4 58 4.00E-4
17 SGTC_2058 5236698 3.34 133.0 µM 8.88E-4 47 4.26E-4
18 SGTC_1819 st053448 3.33 58.6 µM 9.07E-4 amide catabolism 40 4.36E-4
19 SGTC_1431 4049-0260 3.31 9.1 µM 9.78E-4 16 4.75E-4
20 SGTC_1896 st058441 3.29 89.0 µM 0.00102 30 4.95E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 3.06E-24 YPR037C ERV2 hom Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER
0.119 4.75E-12 YDR428C BNA7 hom Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
0.118 7.08E-12 YIL086C_d YIL086C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.110 1.45E-10 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.107 6.49E-10 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.101 4.60E-9 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.088 3.01E-7 YLR142W PUT1 hom Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
0.088 3.26E-7 YPL023C MET12 hom Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR
0.082 2.17E-6 YMR304W UBP15 hom Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p
0.078 5.61E-6 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.078 5.85E-6 YKL201C MNN4 hom Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.078 6.95E-6 YLR311C_d YLR311C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.077 8.26E-6 YBR176W ECM31 hom Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
0.076 9.97E-6 YDL015C TSC13 het Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
0.075 1.27E-5 YNR061C YNR061C hom Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress