YDR446W / ECM11

Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR446W / ECM11

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:3.21|P-value:6.79E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:2.68|P-value:7.20E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.68|P-value:6.29E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.08|P-value:1.28E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.74|P-value:6.28E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.76|P-value:3.97E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:8.05|P-value:7.09E-22 ID:SGTC_171|Compound:k064-0041|FD-Score:-5.04|P-value:1.35E-9 ID:SGTC_177|Compound:1327-0068|FD-Score:-3.86|P-value:2.71E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:2.69|P-value:6.98E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.21|P-value:7.88E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:2.61|P-value:9.48E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.08|P-value:1.25E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:2.63|P-value:8.87E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:2.61|P-value:9.65E-4 ID:SGTC_918|Compound:glabranin|FD-Score:2.63|P-value:8.84E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:-4.08|P-value:7.54E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:2.77|P-value:5.02E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.06|P-value:1.37E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.68|P-value:7.88E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-2.82|P-value:4.56E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.64|P-value:1.92E-8 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.99|P-value:1.28E-6 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.96|P-value:2.41E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-2.66|P-value:8.69E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.14|P-value:9.58E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:2.72|P-value:6.12E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-2.83|P-value:4.31E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.53|P-value:1.39E-5 ID:SGTC_2068|Compound:5226020|FD-Score:2.88|P-value:3.16E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-2.95|P-value:2.56E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-2.65|P-value:8.92E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-3.39|P-value:3.18E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-2.89|P-value:3.25E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.84|P-value:3.75E-4 ID:SGTC_2646|Compound:retinol|FD-Score:2.92|P-value:2.58E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:-3.71|P-value:6.13E-6 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.65|P-value:8.60E-6 ID:SGTC_2903|Compound:5923464|FD-Score:2.81|P-value:4.22E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.02|P-value:1.83E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-2.63|P-value:9.67E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.36|P-value:1.09E-10 ID:SGTC_2998|Compound:9066762|FD-Score:-3.00|P-value:2.01E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.70|P-value:6.44E-6 ID:SGTC_3068|Compound:9116768|FD-Score:-3.37|P-value:3.51E-5 ID:SGTC_3104|Compound:9120930|FD-Score:-3.40|P-value:3.06E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-2.72|P-value:6.81E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.21|P-value:6.79E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:2.68|P-value:7.20E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.68|P-value:6.29E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.08|P-value:1.28E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.74|P-value:6.28E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.76|P-value:3.97E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:8.05|P-value:7.09E-22 ID:SGTC_171|Compound:k064-0041|FD-Score:-5.04|P-value:1.35E-9 ID:SGTC_177|Compound:1327-0068|FD-Score:-3.86|P-value:2.71E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:2.69|P-value:6.98E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.21|P-value:7.88E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:2.61|P-value:9.48E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.08|P-value:1.25E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:2.63|P-value:8.87E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:2.61|P-value:9.65E-4 ID:SGTC_918|Compound:glabranin|FD-Score:2.63|P-value:8.84E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:-4.08|P-value:7.54E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:2.77|P-value:5.02E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.06|P-value:1.37E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.68|P-value:7.88E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-2.82|P-value:4.56E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.64|P-value:1.92E-8 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.99|P-value:1.28E-6 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.96|P-value:2.41E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-2.66|P-value:8.69E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.14|P-value:9.58E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:2.72|P-value:6.12E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-2.83|P-value:4.31E-4 ID:SGTC_1678|Compound:st019265|FD-Score:3.53|P-value:1.39E-5 ID:SGTC_2068|Compound:5226020|FD-Score:2.88|P-value:3.16E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-2.95|P-value:2.56E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-2.65|P-value:8.92E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-3.39|P-value:3.18E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-2.89|P-value:3.25E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.84|P-value:3.75E-4 ID:SGTC_2646|Compound:retinol|FD-Score:2.92|P-value:2.58E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:-3.71|P-value:6.13E-6 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.65|P-value:8.60E-6 ID:SGTC_2903|Compound:5923464|FD-Score:2.81|P-value:4.22E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.02|P-value:1.83E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-2.63|P-value:9.67E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.36|P-value:1.09E-10 ID:SGTC_2998|Compound:9066762|FD-Score:-3.00|P-value:2.01E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.70|P-value:6.44E-6 ID:SGTC_3068|Compound:9116768|FD-Score:-3.37|P-value:3.51E-5 ID:SGTC_3104|Compound:9120930|FD-Score:-3.40|P-value:3.06E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-2.72|P-value:6.81E-4

Top fitness defect scores for YDR446W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_159 3970-0959 8.05 50.0 µM 7.09E-22 13 3.99E-16
2 SGTC_2989 9073649 5.36 71.4 µM 1.09E-10 12 4.27E-8
3 SGTC_1110 3-hydroxy-3'-methoxyflavone 4.64 1.2 µM 1.92E-8 iron homeostasis 28 1.77E-6
4 SGTC_149 3970-0822 3.76 46.1 µM 3.97E-6 tubulin folding & SWR complex 105 8.34E-5
5 SGTC_96 0929-0093 3.68 37.3 µM 6.29E-6 calcium & mitochondrial duress 20 1.16E-4
6 SGTC_1678 st019265 3.53 80.2 µM 1.39E-5 60S ribosome export 33 2.06E-4
7 SGTC_44 0929-0055 3.21 61.2 µM 6.79E-5 61 6.54E-4
8 SGTC_1567 st069303 3.14 68.4 µM 9.58E-5 mitochondrial processes 52 8.40E-4
9 SGTC_532 1548-0774 3.08 215.0 µM 1.25E-4 47 0.00102
10 SGTC_143 4032-1194 3.08 15.7 µM 1.28E-4 131 0.00104
11 SGTC_1063 disulfiram 3.06 1.8 µM 1.37E-4 69 0.00109
12 SGTC_2646 retinol 2.92 96.8 µM 2.58E-4 48 0.00173
13 SGTC_2068 5226020 2.88 147.0 µM 3.16E-4 89 0.00201
14 SGTC_2563 tomatine 2.84 8.5 µM 3.75E-4 131 0.00227
15 SGTC_2903 5923464 2.81 63.9 µM 4.22E-4 38 0.00248
16 SGTC_1053 3366-3204 2.77 134.0 µM 5.02E-4 cell wall 101 0.00281
17 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 2.72 54.9 µM 6.12E-4 redox potentiating 118 0.00325
18 SGTC_214 4373-3496 2.69 70.0 µM 6.98E-4 169 0.00357
19 SGTC_70 0141-0289 2.68 43.3 µM 7.20E-4 mitochondrial stress 339 0.00366
20 SGTC_918 glabranin 2.63 770.7 nM 8.84E-4 113 0.00425

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 6.11E-39 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.198 4.74E-31 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
0.154 3.65E-19 YKL186C MTR2 het mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
0.146 1.97E-17 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.141 1.89E-16 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.135 4.06E-15 YLR352W_p YLR352W_p hom Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene
0.135 4.00E-15 YPL036W PMA2 hom Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
0.133 1.29E-14 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.131 3.05E-14 YML035C AMD1 hom AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
0.129 5.87E-14 YDR022C ATG31 hom Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion
0.129 6.67E-14 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.127 1.48E-13 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.125 4.23E-13 YGR084C MRP13 hom Mitochondrial ribosomal protein of the small subunit
0.123 9.73E-13 YLL039C UBI4 hom Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress
0.122 1.18E-12 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes