YDR458C / HEH2

Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR458C / HEH2

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.18|P-value:9.00E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.88|P-value:8.23E-7 ID:SGTC_259|Compound:usnic acid|FD-Score:3.40|P-value:4.20E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.46|P-value:3.50E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.31|P-value:5.92E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.27|P-value:6.05E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.05|P-value:3.50E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.78|P-value:1.04E-4 ID:SGTC_469|Compound:w7|FD-Score:4.06|P-value:3.41E-5 ID:SGTC_470|Compound:aa-861|FD-Score:5.88|P-value:3.92E-9 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.79|P-value:1.24E-11 ID:SGTC_508|Compound:roscovitine|FD-Score:3.75|P-value:1.17E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.20|P-value:1.84E-5 ID:SGTC_512|Compound:prima-1|FD-Score:6.81|P-value:1.09E-11 ID:SGTC_549|Compound:1661-1316|FD-Score:-3.49|P-value:2.82E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:3.88|P-value:7.17E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:3.33|P-value:5.47E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.89|P-value:6.82E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.12|P-value:2.42E-7 ID:SGTC_867|Compound:0518-0118|FD-Score:4.57|P-value:3.63E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:3.25|P-value:7.23E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-4.32|P-value:1.00E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:-4.02|P-value:3.56E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.89|P-value:7.01E-7 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.59|P-value:1.94E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:-3.51|P-value:2.59E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.16|P-value:9.66E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.53|P-value:2.68E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.18|P-value:8.34E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:5.01|P-value:4.39E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:7.34|P-value:2.69E-13 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.99|P-value:4.57E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.28|P-value:6.59E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:5.39|P-value:5.89E-8 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.87|P-value:8.55E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:3.38|P-value:4.66E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.98|P-value:4.14E-5 ID:SGTC_1530|Compound:rhein|FD-Score:3.31|P-value:5.78E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.66|P-value:1.63E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.34|P-value:5.30E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-4.08|P-value:2.79E-5 ID:SGTC_1643|Compound:st009866|FD-Score:-3.19|P-value:8.08E-4 ID:SGTC_1649|Compound:st011737|FD-Score:-3.34|P-value:4.86E-4 ID:SGTC_1650|Compound:st011916|FD-Score:-4.50|P-value:4.37E-6 ID:SGTC_1668|Compound:st014914|FD-Score:3.63|P-value:1.82E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.82|P-value:1.13E-6 ID:SGTC_1984|Compound:st072354|FD-Score:3.76|P-value:1.12E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.34|P-value:5.25E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-4.75|P-value:1.35E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.58|P-value:2.22E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.61|P-value:1.76E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.45|P-value:3.62E-4 ID:SGTC_2213|Compound:7173844|FD-Score:3.47|P-value:3.38E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:4.81|P-value:1.19E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.25|P-value:7.09E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.64|P-value:1.76E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.31|P-value:5.80E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.73|P-value:1.26E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.89|P-value:7.74E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.15|P-value:2.28E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.17|P-value:1.87E-5 ID:SGTC_2693|Compound:st077581|FD-Score:7.25|P-value:5.20E-13 ID:SGTC_3040|Compound:9091894|FD-Score:4.63|P-value:2.72E-6 ID:SGTC_3283|Compound:9109332|FD-Score:4.03|P-value:3.86E-5 ID:SGTC_3310|Compound:9130229|FD-Score:4.47|P-value:5.65E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:3.18|P-value:9.00E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.88|P-value:8.23E-7 ID:SGTC_259|Compound:usnic acid|FD-Score:3.40|P-value:4.20E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.46|P-value:3.50E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.31|P-value:5.92E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.27|P-value:6.05E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.05|P-value:3.50E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.78|P-value:1.04E-4 ID:SGTC_469|Compound:w7|FD-Score:4.06|P-value:3.41E-5 ID:SGTC_470|Compound:aa-861|FD-Score:5.88|P-value:3.92E-9 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.79|P-value:1.24E-11 ID:SGTC_508|Compound:roscovitine|FD-Score:3.75|P-value:1.17E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.20|P-value:1.84E-5 ID:SGTC_512|Compound:prima-1|FD-Score:6.81|P-value:1.09E-11 ID:SGTC_549|Compound:1661-1316|FD-Score:-3.49|P-value:2.82E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:3.88|P-value:7.17E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:3.33|P-value:5.47E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.89|P-value:6.82E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.12|P-value:2.42E-7 ID:SGTC_867|Compound:0518-0118|FD-Score:4.57|P-value:3.63E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:3.25|P-value:7.23E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:-4.32|P-value:1.00E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:-4.02|P-value:3.56E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.89|P-value:7.01E-7 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.59|P-value:1.94E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:-3.51|P-value:2.59E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.16|P-value:9.66E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.53|P-value:2.68E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.18|P-value:8.34E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:5.01|P-value:4.39E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:7.34|P-value:2.69E-13 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.99|P-value:4.57E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.28|P-value:6.59E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:5.39|P-value:5.89E-8 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.87|P-value:8.55E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:3.38|P-value:4.66E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.98|P-value:4.14E-5 ID:SGTC_1530|Compound:rhein|FD-Score:3.31|P-value:5.78E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.66|P-value:1.63E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.34|P-value:5.30E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-4.08|P-value:2.79E-5 ID:SGTC_1643|Compound:st009866|FD-Score:-3.19|P-value:8.08E-4 ID:SGTC_1649|Compound:st011737|FD-Score:-3.34|P-value:4.86E-4 ID:SGTC_1650|Compound:st011916|FD-Score:-4.50|P-value:4.37E-6 ID:SGTC_1668|Compound:st014914|FD-Score:3.63|P-value:1.82E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.82|P-value:1.13E-6 ID:SGTC_1984|Compound:st072354|FD-Score:3.76|P-value:1.12E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.34|P-value:5.25E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-4.75|P-value:1.35E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.58|P-value:2.22E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.61|P-value:1.76E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.45|P-value:3.62E-4 ID:SGTC_2213|Compound:7173844|FD-Score:3.47|P-value:3.38E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:4.81|P-value:1.19E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.25|P-value:7.09E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.64|P-value:1.76E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.31|P-value:5.80E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.73|P-value:1.26E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.89|P-value:7.74E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.15|P-value:2.28E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.17|P-value:1.87E-5 ID:SGTC_2693|Compound:st077581|FD-Score:7.25|P-value:5.20E-13 ID:SGTC_3040|Compound:9091894|FD-Score:4.63|P-value:2.72E-6 ID:SGTC_3283|Compound:9109332|FD-Score:4.03|P-value:3.86E-5 ID:SGTC_3310|Compound:9130229|FD-Score:4.47|P-value:5.65E-6

Top fitness defect scores for YDR458C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1290 0986-0249 7.34 10.2 µM 2.69E-13 DNA intercalators 4 1.06E-13
2 SGTC_2693 st077581 7.25 40.7 µM 5.20E-13 2 2.10E-13
3 SGTC_512 prima-1 6.81 270.0 µM 1.09E-11 DNA intercalators 3 4.87E-12
4 SGTC_471 1-acyl-paf 6.79 4.0 µM 1.24E-11 DNA intercalators 4 5.55E-12
5 SGTC_470 aa-861 5.88 153.0 µM 3.92E-9 sphingolipid biosynthesis & PDR1 23 2.11E-9
6 SGTC_1415 3966-0071 5.39 78.1 µM 5.89E-8 DNA intercalators 1 3.47E-8
7 SGTC_682 0kpi-0099 5.12 17.0 µM 2.42E-7 35 1.49E-7
8 SGTC_1280 0880-0253 5.01 10.1 µM 4.39E-7 3 2.76E-7
9 SGTC_2604 4'-hydroxychalcone 4.89 11.3 µM 7.74E-7 19 4.96E-7
10 SGTC_184 1494-0393 4.88 24.7 µM 8.23E-7 35 5.29E-7
11 SGTC_1459 k035-0031 4.87 35.7 µM 8.55E-7 DNA intercalators 9 5.50E-7
12 SGTC_1926 methyl fluorone black 4.82 53.6 µM 1.13E-6 DNA intercalators 38 7.33E-7
13 SGTC_2576 guaiol 4.81 23.1 µM 1.19E-6 7 7.71E-7
14 SGTC_3040 9091894 4.63 49.5 µM 2.72E-6 DNA intercalators 13 1.82E-6
15 SGTC_867 0518-0118 4.57 155.0 µM 3.63E-6 DNA intercalators 12 2.44E-6
16 SGTC_3310 9130229 4.47 44.1 µM 5.65E-6 Golgi 13 3.87E-6
17 SGTC_511 blebbistatin 4.20 171.0 µM 1.84E-5 DNA intercalators 17 1.31E-5
18 SGTC_2611 pyromycin 4.15 23.9 µM 2.28E-5 21 1.63E-5
19 SGTC_469 w7 4.06 172.0 µM 3.41E-5 ERG2 10 2.48E-5
20 SGTC_398 bronopol 4.05 33.3 µM 3.50E-5 RNA processing & uracil transport 25 2.54E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.343 5.31E-93 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.298 7.17E-70 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.293 2.29E-67 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.290 4.78E-66 YDL058W USO1 het Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.290 6.09E-66 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.275 3.08E-59 YDR362C TFC6 het One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
0.257 1.71E-51 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.251 6.09E-49 YMR119W ASI1 hom Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
0.246 2.04E-47 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.232 5.38E-42 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.231 7.47E-42 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.231 9.29E-42 YNL317W PFS2 het Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
0.215 3.19E-36 YOR278W HEM4 het Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
0.208 4.38E-34 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.202 3.12E-32 YGL023C PIB2 hom Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27