YDR466W / PKH3

Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR466W / PKH3

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:-4.16|P-value:5.04E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.47|P-value:2.20E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.55|P-value:1.14E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:5.82|P-value:2.32E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.90|P-value:5.94E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:8.92|P-value:1.14E-12 ID:SGTC_114|Compound:0364-0001|FD-Score:6.19|P-value:5.62E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-5.42|P-value:9.22E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.31|P-value:3.55E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.28|P-value:3.87E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.37|P-value:2.70E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.41|P-value:9.57E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.35|P-value:3.16E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:11.50|P-value:5.53E-20 ID:SGTC_681|Compound:1642-0006|FD-Score:4.51|P-value:1.98E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:4.42|P-value:2.54E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.15|P-value:5.63E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.66|P-value:1.24E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:4.33|P-value:3.31E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:5.38|P-value:1.15E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:14.70|P-value:3.26E-31 ID:SGTC_1498|Compound:4478-3555|FD-Score:6.10|P-value:8.03E-7 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.98|P-value:8.79E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:6.75|P-value:5.49E-8 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.05|P-value:9.68E-7 ID:SGTC_1625|Compound:st003711|FD-Score:4.27|P-value:3.97E-4 ID:SGTC_1627|Compound:st003713|FD-Score:13.70|P-value:1.37E-27 ID:SGTC_1629|Compound:st005085|FD-Score:4.48|P-value:2.12E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-6.60|P-value:9.39E-8 ID:SGTC_1712|Compound:st032288|FD-Score:11.20|P-value:6.87E-19 ID:SGTC_1768|Compound:st044301|FD-Score:4.56|P-value:1.67E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.62|P-value:1.41E-4 ID:SGTC_2021|Compound:4019055|FD-Score:10.10|P-value:9.93E-16 ID:SGTC_2059|Compound:5238311|FD-Score:4.76|P-value:9.01E-5 ID:SGTC_2267|Compound:7931562|FD-Score:4.70|P-value:1.11E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.84|P-value:6.62E-5 ID:SGTC_2364|Compound:9055074|FD-Score:4.18|P-value:5.10E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.60|P-value:1.49E-4 ID:SGTC_2482|Compound:5981709|FD-Score:4.04|P-value:7.58E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.60|P-value:1.48E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:5.17|P-value:2.42E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.66|P-value:1.15E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:18.10|P-value:3.56E-46 ID:SGTC_2777|Compound:climbazole|FD-Score:4.26|P-value:4.10E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.13|P-value:5.93E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.22|P-value:2.04E-5 ID:SGTC_2973|Compound:9094665|FD-Score:4.39|P-value:2.77E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.95|P-value:9.47E-4 ID:SGTC_3057|Compound:9095946|FD-Score:4.11|P-value:6.25E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-5.22|P-value:1.85E-5 ID:SGTC_3144|Compound:9087057|FD-Score:-5.68|P-value:3.59E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-4.08|P-value:6.37E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-4.16|P-value:5.04E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.47|P-value:2.20E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.55|P-value:1.14E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:5.82|P-value:2.32E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.90|P-value:5.94E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:8.92|P-value:1.14E-12 ID:SGTC_114|Compound:0364-0001|FD-Score:6.19|P-value:5.62E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-5.42|P-value:9.22E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.31|P-value:3.55E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.28|P-value:3.87E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.37|P-value:2.70E-7 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.41|P-value:9.57E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.35|P-value:3.16E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:11.50|P-value:5.53E-20 ID:SGTC_681|Compound:1642-0006|FD-Score:4.51|P-value:1.98E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:4.42|P-value:2.54E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.15|P-value:5.63E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.66|P-value:1.24E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:4.33|P-value:3.31E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:5.38|P-value:1.15E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:14.70|P-value:3.26E-31 ID:SGTC_1498|Compound:4478-3555|FD-Score:6.10|P-value:8.03E-7 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.98|P-value:8.79E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:6.75|P-value:5.49E-8 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.05|P-value:9.68E-7 ID:SGTC_1625|Compound:st003711|FD-Score:4.27|P-value:3.97E-4 ID:SGTC_1627|Compound:st003713|FD-Score:13.70|P-value:1.37E-27 ID:SGTC_1629|Compound:st005085|FD-Score:4.48|P-value:2.12E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-6.60|P-value:9.39E-8 ID:SGTC_1712|Compound:st032288|FD-Score:11.20|P-value:6.87E-19 ID:SGTC_1768|Compound:st044301|FD-Score:4.56|P-value:1.67E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.62|P-value:1.41E-4 ID:SGTC_2021|Compound:4019055|FD-Score:10.10|P-value:9.93E-16 ID:SGTC_2059|Compound:5238311|FD-Score:4.76|P-value:9.01E-5 ID:SGTC_2267|Compound:7931562|FD-Score:4.70|P-value:1.11E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.84|P-value:6.62E-5 ID:SGTC_2364|Compound:9055074|FD-Score:4.18|P-value:5.10E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.60|P-value:1.49E-4 ID:SGTC_2482|Compound:5981709|FD-Score:4.04|P-value:7.58E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.60|P-value:1.48E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:5.17|P-value:2.42E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.66|P-value:1.15E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:18.10|P-value:3.56E-46 ID:SGTC_2777|Compound:climbazole|FD-Score:4.26|P-value:4.10E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.13|P-value:5.93E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.22|P-value:2.04E-5 ID:SGTC_2973|Compound:9094665|FD-Score:4.39|P-value:2.77E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.95|P-value:9.47E-4 ID:SGTC_3057|Compound:9095946|FD-Score:4.11|P-value:6.25E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-5.22|P-value:1.85E-5 ID:SGTC_3144|Compound:9087057|FD-Score:-5.68|P-value:3.59E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-4.08|P-value:6.37E-4

Top fitness defect scores for YDR466W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 18.10 96.7 µM 3.56E-46 calcium & mitochondrial duress 2 3.63E-73
2 SGTC_1495 4469-0029 14.70 237.0 µM 3.26E-31 5 4.16E-49
3 SGTC_1627 st003713 13.70 25.6 µM 1.37E-27 calcium & mitochondrial duress 4 2.77E-43
4 SGTC_528 3492-0019 11.50 172.0 µM 5.53E-20 calcium & mitochondrial duress 31 4.54E-31
5 SGTC_1712 st032288 11.20 53.4 µM 6.87E-19 calcium & mitochondrial duress 17 2.59E-29
6 SGTC_2021 4019055 10.10 173.0 µM 9.93E-16 endomembrane recycling 9 3.03E-24
7 SGTC_108 0509-0257 8.92 621.1 µM 1.14E-12 4 2.43E-19
8 SGTC_1502 4534-4282 6.75 27.7 µM 5.49E-8 2 7.38E-12
9 SGTC_467 ro 31-8220 6.37 72.8 µM 2.70E-7 calcium & mitochondrial duress 28 9.33E-11
10 SGTC_114 0364-0001 6.19 490.3 µM 5.62E-7 9 3.00E-10
11 SGTC_1498 4478-3555 6.10 97.6 µM 8.03E-7 1 5.29E-10
12 SGTC_1597 7-benzyloxygramine 6.05 47.5 µM 9.68E-7 calcium & mitochondrial duress 48 7.13E-10
13 SGTC_65 0798-0905 5.82 35.0 µM 2.32E-6 calcium & mitochondrial duress 35 2.87E-9
14 SGTC_1476 4112-3294 5.38 3.3 µM 1.15E-5 60S ribosome export 3 3.65E-8
15 SGTC_2896 9052656 5.22 71.4 µM 2.04E-5 endomembrane recycling 53 8.98E-8
16 SGTC_2552 juarezic acid 5.17 87.0 µM 2.42E-5 TSC3-RPN4 11 1.18E-7
17 SGTC_70 0141-0289 4.90 43.3 µM 5.94E-5 mitochondrial stress 113 4.89E-7
18 SGTC_2059 5238311 4.76 157.0 µM 9.01E-5 8 9.45E-7
19 SGTC_2267 7931562 4.70 200.0 µM 1.11E-4 6 1.31E-6
20 SGTC_1275 0867-0008 4.66 21.1 µM 1.24E-4 7 1.57E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.358 3.37E-102 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.324 1.08E-82 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.305 2.17E-73 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.289 1.55E-65 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.287 1.46E-64 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.268 3.03E-56 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.268 3.34E-56 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.259 9.47E-53 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.240 2.70E-45 YNL086W_p SNN1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes
0.239 1.20E-44 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.238 1.44E-44 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.238 2.36E-44 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.237 4.06E-44 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.231 1.04E-41 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.230 2.00E-41 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes