YDR481C / PHO8

Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR481C / PHO8

Click on Significant Values for Screen Details ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.24|P-value:1.03E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:3.61|P-value:1.56E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:3.87|P-value:5.28E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:-2.99|P-value:8.13E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.25|P-value:6.01E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.31|P-value:2.51E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.65|P-value:1.30E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.07|P-value:6.14E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.35|P-value:4.12E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-3.26|P-value:3.08E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.20|P-value:1.27E-5 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.14|P-value:8.67E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.26|P-value:5.63E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.74|P-value:9.24E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.13|P-value:9.19E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.82|P-value:6.46E-5 ID:SGTC_1673|Compound:st016611|FD-Score:7.33|P-value:5.91E-14 ID:SGTC_1675|Compound:st018485|FD-Score:3.64|P-value:1.36E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.80|P-value:7.20E-5 ID:SGTC_1740|Compound:st038286|FD-Score:4.90|P-value:4.05E-7 ID:SGTC_1748|Compound:st037455|FD-Score:3.89|P-value:4.82E-5 ID:SGTC_1749|Compound:st037932|FD-Score:8.55|P-value:2.10E-18 ID:SGTC_2055|Compound:5235184|FD-Score:-4.51|P-value:1.27E-6 ID:SGTC_2311|Compound:7694451|FD-Score:-2.98|P-value:8.66E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.53|P-value:2.05E-4 ID:SGTC_2395|Compound:5646831|FD-Score:5.71|P-value:4.24E-9 ID:SGTC_2397|Compound:5161689|FD-Score:5.02|P-value:2.13E-7 ID:SGTC_2403|Compound:6032726|FD-Score:-3.35|P-value:2.18E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.68|P-value:1.14E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.13|P-value:1.68E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.24|P-value:6.10E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.70|P-value:5.40E-5 ID:SGTC_2713|Compound:st079149|FD-Score:3.52|P-value:2.17E-4 ID:SGTC_2806|Compound:7952793|FD-Score:3.68|P-value:1.17E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.87|P-value:5.31E-5 ID:SGTC_3073|Compound:9114972|FD-Score:-3.38|P-value:1.92E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.15|P-value:8.49E-4 ID:SGTC_3272|Compound:9137413|FD-Score:4.28|P-value:8.50E-6 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.24|P-value:1.03E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:3.61|P-value:1.56E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:3.87|P-value:5.28E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:-2.99|P-value:8.13E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.25|P-value:6.01E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.31|P-value:2.51E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.65|P-value:1.30E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.07|P-value:6.14E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.35|P-value:4.12E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:-3.26|P-value:3.08E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.20|P-value:1.27E-5 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.14|P-value:8.67E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.26|P-value:5.63E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.74|P-value:9.24E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.13|P-value:9.19E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.82|P-value:6.46E-5 ID:SGTC_1673|Compound:st016611|FD-Score:7.33|P-value:5.91E-14 ID:SGTC_1675|Compound:st018485|FD-Score:3.64|P-value:1.36E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.80|P-value:7.20E-5 ID:SGTC_1740|Compound:st038286|FD-Score:4.90|P-value:4.05E-7 ID:SGTC_1748|Compound:st037455|FD-Score:3.89|P-value:4.82E-5 ID:SGTC_1749|Compound:st037932|FD-Score:8.55|P-value:2.10E-18 ID:SGTC_2055|Compound:5235184|FD-Score:-4.51|P-value:1.27E-6 ID:SGTC_2311|Compound:7694451|FD-Score:-2.98|P-value:8.66E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.53|P-value:2.05E-4 ID:SGTC_2395|Compound:5646831|FD-Score:5.71|P-value:4.24E-9 ID:SGTC_2397|Compound:5161689|FD-Score:5.02|P-value:2.13E-7 ID:SGTC_2403|Compound:6032726|FD-Score:-3.35|P-value:2.18E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.68|P-value:1.14E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.13|P-value:1.68E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.24|P-value:6.10E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.70|P-value:5.40E-5 ID:SGTC_2713|Compound:st079149|FD-Score:3.52|P-value:2.17E-4 ID:SGTC_2806|Compound:7952793|FD-Score:3.68|P-value:1.17E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.87|P-value:5.31E-5 ID:SGTC_3073|Compound:9114972|FD-Score:-3.38|P-value:1.92E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.15|P-value:8.49E-4 ID:SGTC_3272|Compound:9137413|FD-Score:4.28|P-value:8.50E-6

Top fitness defect scores for YDR481C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1749 st037932 8.55 54.2 µM 2.10E-18 1 5.93E-18
2 SGTC_1673 st016611 7.33 27.3 µM 5.91E-14 11 1.16E-13
3 SGTC_2395 5646831 5.71 180.2 µM 4.24E-9 8 5.72E-9
4 SGTC_2397 5161689 5.02 200.0 µM 2.13E-7 15 2.55E-7
5 SGTC_1740 st038286 4.90 13.2 µM 4.05E-7 12 4.76E-7
6 SGTC_3272 9137413 4.28 49.5 µM 8.50E-6 9 9.16E-6
7 SGTC_390 dec-rvkr-cmk 4.24 16.7 µM 1.03E-5 calcium & mitochondrial duress 17 1.10E-5
8 SGTC_1308 1187-1617 4.20 41.4 µM 1.27E-5 fatty acid desaturase (OLE1) 32 1.35E-5
9 SGTC_2451 5856223 4.13 45.7 µM 1.68E-5 21 1.77E-5
10 SGTC_1748 st037455 3.89 53.6 µM 4.82E-5 35 4.96E-5
11 SGTC_665 3914-0008 3.87 307.0 µM 5.28E-5 21 5.43E-5
12 SGTC_2936 9038235 3.87 15.6 µM 5.31E-5 19 5.45E-5
13 SGTC_1658 st012944 3.82 20.0 µM 6.46E-5 iron homeostasis 81 6.61E-5
14 SGTC_1718 roemerine 3.80 11.9 µM 7.20E-5 redox potentiating 61 7.35E-5
15 SGTC_1592 4',5'-dihydroxyflavone 3.74 8.8 µM 9.24E-5 60S ribosome export 17 9.37E-5
16 SGTC_2437 5741531 3.68 144.2 µM 1.14E-4 44 1.15E-4
17 SGTC_2806 7952793 3.68 58.4 µM 1.17E-4 33 1.18E-4
18 SGTC_1010 4112-3296 3.65 15.2 µM 1.30E-4 24 1.31E-4
19 SGTC_1675 st018485 3.64 24.5 µM 1.36E-4 60S ribosome export 38 1.37E-4
20 SGTC_626 0214-0009 3.61 33.0 µM 1.56E-4 copper-dependent oxidative stress 30 1.56E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 3.87E-23 YMR101C SRT1 hom Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
0.156 9.89E-20 YAL018C_p YAL018C_p hom Putative protein of unknown function
0.123 7.12E-13 YHR015W MIP6 hom Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.118 7.41E-12 YGR144W THI4 hom Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.111 1.20E-10 YBL094C_d YBL094C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
0.106 8.04E-10 YHL010C ETP1 hom Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences
0.106 8.33E-10 YMR254C_d YMR254C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.104 1.46E-9 YOL106W_d YOL106W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 6.63E-8 YHR127W YHR127W hom Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
0.092 8.25E-8 YLR090W XDJ1 hom Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.090 1.85E-7 YGL166W CUP2 hom Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
0.088 2.98E-7 YER114C BOI2 hom Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
0.085 7.99E-7 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.085 8.79E-7 YDR224C HTB1 het Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.084 9.71E-7 YOL017W ESC8 hom Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication