HIPHOP chemogenomics database

YDR486C / VPS60

Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR486C / VPS60

Click on Significant Values for Screen Details

Top fitness defect scores for YDR486C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2322	7413319	10.20	38.2 µM	5.06E-23	ERAD & cell cycle	18	8.01E-25
2	SGTC_568	1193-0097	6.78	7.5 µM	3.88E-11	ERAD & cell cycle	50	6.06E-12
3	SGTC_1060	3448-7350	6.39	82.0 µM	4.43E-10	Golgi	11	8.48E-11
4	SGTC_1495	4469-0029	6.29	237.0 µM	7.90E-10		45	1.59E-10
5	SGTC_2249	haloperidol	6.29	116.4 µM	7.91E-10	ergosterol depletion effects on membrane	39	1.59E-10
6	SGTC_525	k781-2675	5.93	26.7 µM	6.19E-9	redox potentiating	26	1.47E-9
7	SGTC_951	1171-0579	5.66	82.7 µM	2.76E-8	cell wall	37	7.45E-9
8	SGTC_300	r073-0011	4.81	5.0 µM	2.01E-6		7	7.73E-7
9	SGTC_2038	4100038	4.68	31.0 µM	3.56E-6	plasma membrane duress	29	1.43E-6
10	SGTC_2804	7954240	4.56	19.5 µM	6.01E-6		21	2.52E-6
11	SGTC_255	tunicamycin	4.51	200.5 nM	7.42E-6	unfolded protein response	53	3.17E-6
12	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	4.36	54.9 µM	1.47E-5	redox potentiating	45	6.62E-6
13	SGTC_2338	7999974	4.35	200.0 µM	1.53E-5		11	6.92E-6
14	SGTC_1658	st012944	4.32	20.0 µM	1.74E-5	iron homeostasis	67	7.97E-6
15	SGTC_37	0566-0070	4.29	1.6 µM	1.96E-5		19	9.04E-6
16	SGTC_2021	4019055	4.27	173.0 µM	2.14E-5	endomembrane recycling	104	9.97E-6
17	SGTC_2334	9017922	4.26	200.0 µM	2.16E-5		17	1.01E-5
18	SGTC_2050	5263056	4.26	171.0 µM	2.22E-5	fatty acid desaturase (OLE1)	35	1.04E-5
19	SGTC_351	caspofungin	4.09	25.0 nM	4.29E-5	cell wall	89	2.12E-5
20	SGTC_1183	1254-0324	4.02	198.0 µM	5.80E-5		20	2.93E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.253	3.87E-50	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.245	4.23E-47	YKL041W	VPS24	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.234	6.68E-43	YAL002W	VPS8	hom	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.219	1.34E-37	YPR173C	VPS4	hom	AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.209	2.11E-34	YOR068C	VAM10	hom	Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
0.206	1.85E-33	YPL065W	VPS28	hom	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.199	3.51E-31	YDR495C	VPS3	hom	Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.198	4.72E-31	YNR006W	VPS27	hom	Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.198	4.94E-31	YOR089C	VPS21	hom	Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.194	1.03E-29	YKR035W-A	DID2	hom	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.190	1.69E-28	YKL002W	DID4	hom	Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.188	4.83E-28	YLR119W	SRN2	hom	Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.185	3.45E-27	YNR005C_d	YNR005C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184	5.12E-27	YLR417W	VPS36	hom	Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.181	3.51E-26	YKR035C_d	OPI8_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A