YDR532C / KRE28

Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR532C / KRE28

Click on Significant Values for Screen Details ID:SGTC_211|Compound:3455-0679|FD-Score:-4.35|P-value:3.86E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.85|P-value:6.33E-6 ID:SGTC_651|Compound:3253-0966|FD-Score:-4.41|P-value:3.09E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:3.52|P-value:7.76E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-3.40|P-value:9.76E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-4.57|P-value:1.64E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.10|P-value:1.11E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-5.30|P-value:7.24E-7 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.49|P-value:7.44E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:-4.78|P-value:7.01E-6 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.79|P-value:3.26E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.59|P-value:6.31E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.56|P-value:6.74E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.44|P-value:8.63E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-4.24|P-value:5.74E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-4.62|P-value:1.36E-5 ID:SGTC_2074|Compound:5212007|FD-Score:-6.43|P-value:2.61E-9 ID:SGTC_2177|Compound:5811945|FD-Score:-3.41|P-value:9.60E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-5.87|P-value:4.79E-8 ID:SGTC_2760|Compound:etidronate|FD-Score:5.31|P-value:8.68E-7 ID:SGTC_2833|Compound:9001315|FD-Score:4.23|P-value:6.99E-5 ID:SGTC_3012|Compound:9080433|FD-Score:-3.59|P-value:5.38E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.85|P-value:2.67E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:-4.35|P-value:3.86E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.85|P-value:6.33E-6 ID:SGTC_651|Compound:3253-0966|FD-Score:-4.41|P-value:3.09E-5 ID:SGTC_662|Compound:k832-1615|FD-Score:3.52|P-value:7.76E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-3.40|P-value:9.76E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-4.57|P-value:1.64E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.10|P-value:1.11E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-5.30|P-value:7.24E-7 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.49|P-value:7.44E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:-4.78|P-value:7.01E-6 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.79|P-value:3.26E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:3.59|P-value:6.31E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.56|P-value:6.74E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.44|P-value:8.63E-4 ID:SGTC_1861|Compound:st057647|FD-Score:-4.24|P-value:5.74E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-4.62|P-value:1.36E-5 ID:SGTC_2074|Compound:5212007|FD-Score:-6.43|P-value:2.61E-9 ID:SGTC_2177|Compound:5811945|FD-Score:-3.41|P-value:9.60E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-5.87|P-value:4.79E-8 ID:SGTC_2760|Compound:etidronate|FD-Score:5.31|P-value:8.68E-7 ID:SGTC_2833|Compound:9001315|FD-Score:4.23|P-value:6.99E-5 ID:SGTC_3012|Compound:9080433|FD-Score:-3.59|P-value:5.38E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.85|P-value:2.67E-4

Top fitness defect scores for YDR532C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2760 etidronate 5.31 98.0 µM 8.68E-7 5 5.58E-8
2 SGTC_449 xestospongin c 4.85 11.2 µM 6.33E-6 NEO1-PIK1 34 6.27E-7
3 SGTC_2833 9001315 4.23 45.5 µM 6.99E-5 amide catabolism 46 1.16E-5
4 SGTC_1130 3454-2858 4.10 15.9 µM 1.11E-4 NEO1 81 2.05E-5
5 SGTC_3200 9114385 3.85 49.5 µM 2.67E-4 39 5.93E-5
6 SGTC_1497 4476-2978 3.79 41.3 µM 3.26E-4 12 7.54E-5
7 SGTC_1543 azetidyl-2-carboxylic acid 3.59 198.0 µM 6.31E-4 25 1.68E-4
8 SGTC_1557 sumaresinolic acid 3.56 42.3 µM 6.74E-4 28 1.82E-4
9 SGTC_662 k832-1615 3.52 104.0 µM 7.76E-4 35 2.16E-4
10 SGTC_1495 4469-0029 3.41 237.0 µM 0.00107 150 3.20E-4
11 SGTC_2565 isoliquiritigenin 3.30 100.0 µM 0.00151 54 4.85E-4
12 SGTC_982 1491-0518 3.29 27.5 µM 0.00153 36 4.93E-4
13 SGTC_1125 tnp00248 3.17 1.3 µM 0.00220 82 7.62E-4
14 SGTC_1820 st053765 3.07 18.6 µM 0.00289 RPP1 & pyrimidine depletion 57 0.00106
15 SGTC_2773 thioridazine 3.02 9.1 µM 0.00331 42 0.00125
16 SGTC_980 1473-0395 2.99 201.0 µM 0.00357 40 0.00137
17 SGTC_1071 trifluoperazine 2.97 6.3 µM 0.00385 66 0.00150
18 SGTC_2546 rhodinyl acetate 2.96 100.0 µM 0.00392 48 0.00154
19 SGTC_1899 st063254 2.96 59.6 µM 0.00396 44 0.00155
20 SGTC_1661 st013063 2.94 9.7 µM 0.00409 60 0.00162

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.093 6.11E-8 YDR411C DFM1 hom Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
0.093 6.93E-8 YDR105C TMS1 hom Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
0.086 6.84E-7 YMR122C_d YMR122C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.077 8.38E-6 YBR059C AKL1 hom Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization
0.077 8.73E-6 YOR025W HST3 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.073 2.27E-5 YGL097W SRM1 het Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.073 2.42E-5 YGL105W ARC1 hom Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress
0.072 2.95E-5 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.072 3.06E-5 YPL079W RPL21B hom Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
0.072 3.18E-5 YPR020W ATP20 hom Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex
0.069 6.92E-5 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.068 7.30E-5 YDL033C SLM3 hom tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
0.068 7.63E-5 YMR060C SAM37 hom Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
0.067 1.02E-4 YHR021C RPS27B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
0.066 1.41E-4 YDL090C RAM1 hom Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit