HIPHOP chemogenomics database

YDR532C / KRE28

Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR532C / KRE28

Click on Significant Values for Screen Details

Top fitness defect scores for YDR532C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2760	etidronate	5.31	98.0 µM	8.68E-7		5	5.58E-8
2	SGTC_449	xestospongin c	4.85	11.2 µM	6.33E-6	NEO1-PIK1	34	6.27E-7
3	SGTC_2833	9001315	4.23	45.5 µM	6.99E-5	amide catabolism	46	1.16E-5
4	SGTC_1130	3454-2858	4.10	15.9 µM	1.11E-4	NEO1	81	2.05E-5
5	SGTC_3200	9114385	3.85	49.5 µM	2.67E-4		39	5.93E-5
6	SGTC_1497	4476-2978	3.79	41.3 µM	3.26E-4		12	7.54E-5
7	SGTC_1543	azetidyl-2-carboxylic acid	3.59	198.0 µM	6.31E-4		25	1.68E-4
8	SGTC_1557	sumaresinolic acid	3.56	42.3 µM	6.74E-4		28	1.82E-4
9	SGTC_662	k832-1615	3.52	104.0 µM	7.76E-4		35	2.16E-4
10	SGTC_1495	4469-0029	3.41	237.0 µM	0.00107		150	3.20E-4
11	SGTC_2565	isoliquiritigenin	3.30	100.0 µM	0.00151		54	4.85E-4
12	SGTC_982	1491-0518	3.29	27.5 µM	0.00153		36	4.93E-4
13	SGTC_1125	tnp00248	3.17	1.3 µM	0.00220		82	7.62E-4
14	SGTC_1820	st053765	3.07	18.6 µM	0.00289	RPP1 & pyrimidine depletion	57	0.00106
15	SGTC_2773	thioridazine	3.02	9.1 µM	0.00331		42	0.00125
16	SGTC_980	1473-0395	2.99	201.0 µM	0.00357		40	0.00137
17	SGTC_1071	trifluoperazine	2.97	6.3 µM	0.00385		66	0.00150
18	SGTC_2546	rhodinyl acetate	2.96	100.0 µM	0.00392		48	0.00154
19	SGTC_1899	st063254	2.96	59.6 µM	0.00396		44	0.00155
20	SGTC_1661	st013063	2.94	9.7 µM	0.00409		60	0.00162

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.093	6.11E-8	YDR411C	DFM1	hom	Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
0.093	6.93E-8	YDR105C	TMS1	hom	Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
0.086	6.84E-7	YMR122C_d	YMR122C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.077	8.38E-6	YBR059C	AKL1	hom	Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization
0.077	8.73E-6	YOR025W	HST3	hom	Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.073	2.27E-5	YGL097W	SRM1	het	Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.073	2.42E-5	YGL105W	ARC1	hom	Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress
0.072	2.95E-5	YGL071W	AFT1	hom	Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.072	3.06E-5	YPL079W	RPL21B	hom	Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
0.072	3.18E-5	YPR020W	ATP20	hom	Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex
0.069	6.92E-5	YLR396C	VPS33	hom	ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.068	7.30E-5	YDL033C	SLM3	hom	tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
0.068	7.63E-5	YMR060C	SAM37	hom	Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
0.067	1.02E-4	YHR021C	RPS27B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
0.066	1.41E-4	YDL090C	RAM1	hom	Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit