YEL003W / GIM4

Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL003W / GIM4

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-3.94|P-value:7.91E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:3.26|P-value:7.16E-4 ID:SGTC_57|Compound:miconazole|FD-Score:5.61|P-value:2.36E-8 ID:SGTC_91|Compound:0417-1751|FD-Score:3.20|P-value:8.74E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.45|P-value:2.75E-10 ID:SGTC_116|Compound:0155-0182|FD-Score:9.07|P-value:6.42E-19 ID:SGTC_117|Compound:lucigenin|FD-Score:16.70|P-value:3.57E-59 ID:SGTC_123|Compound:0083-0120|FD-Score:-4.00|P-value:6.44E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:5.31|P-value:1.13E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:4.33|P-value:1.18E-5 ID:SGTC_175|Compound:3793-3120|FD-Score:3.43|P-value:4.04E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.84|P-value:8.93E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.63|P-value:2.52E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.92|P-value:6.51E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.43|P-value:1.07E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:3.74|P-value:1.27E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.75|P-value:1.68E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.03|P-value:4.16E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:3.69|P-value:1.54E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.74|P-value:1.72E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-4.71|P-value:3.15E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:4.01|P-value:4.57E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:3.40|P-value:4.48E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:3.28|P-value:6.65E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.35|P-value:6.84E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.30|P-value:1.33E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.58|P-value:2.29E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.74|P-value:1.29E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:5.12|P-value:3.00E-7 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.28|P-value:6.69E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.91|P-value:6.73E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:4.45|P-value:6.97E-6 ID:SGTC_1712|Compound:st032288|FD-Score:5.31|P-value:1.14E-7 ID:SGTC_1884|Compound:5633444|FD-Score:-3.35|P-value:6.74E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.62|P-value:1.99E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.49|P-value:4.25E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.27|P-value:6.80E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.81|P-value:1.38E-6 ID:SGTC_2346|Compound:9031566|FD-Score:4.54|P-value:4.78E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.08|P-value:4.57E-5 ID:SGTC_2452|Compound:5863136|FD-Score:4.52|P-value:5.19E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.93|P-value:8.05E-12 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.19|P-value:8.95E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.15|P-value:1.75E-12 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.75|P-value:2.41E-14 ID:SGTC_2756|Compound:carbadox|FD-Score:3.26|P-value:7.07E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:4.41|P-value:8.36E-6 ID:SGTC_2886|Compound:9058944|FD-Score:3.71|P-value:1.45E-4 ID:SGTC_2891|Compound:9063876|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-9.37|P-value:8.56E-20 ID:SGTC_2935|Compound:9038016|FD-Score:6.48|P-value:1.45E-10 ID:SGTC_3017|Compound:9080681|FD-Score:3.76|P-value:1.21E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.94|P-value:7.91E-5 ID:SGTC_56|Compound:0957-0365|FD-Score:3.26|P-value:7.16E-4 ID:SGTC_57|Compound:miconazole|FD-Score:5.61|P-value:2.36E-8 ID:SGTC_91|Compound:0417-1751|FD-Score:3.20|P-value:8.74E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.45|P-value:2.75E-10 ID:SGTC_116|Compound:0155-0182|FD-Score:9.07|P-value:6.42E-19 ID:SGTC_117|Compound:lucigenin|FD-Score:16.70|P-value:3.57E-59 ID:SGTC_123|Compound:0083-0120|FD-Score:-4.00|P-value:6.44E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:5.31|P-value:1.13E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:4.33|P-value:1.18E-5 ID:SGTC_175|Compound:3793-3120|FD-Score:3.43|P-value:4.04E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.84|P-value:8.93E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.63|P-value:2.52E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.92|P-value:6.51E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.43|P-value:1.07E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:3.74|P-value:1.27E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.75|P-value:1.68E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.03|P-value:4.16E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:3.69|P-value:1.54E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.74|P-value:1.72E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-4.71|P-value:3.15E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:4.01|P-value:4.57E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:3.40|P-value:4.48E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:3.28|P-value:6.65E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.35|P-value:6.84E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.30|P-value:1.33E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.58|P-value:2.29E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.74|P-value:1.29E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:5.12|P-value:3.00E-7 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.28|P-value:6.69E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.91|P-value:6.73E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:4.45|P-value:6.97E-6 ID:SGTC_1712|Compound:st032288|FD-Score:5.31|P-value:1.14E-7 ID:SGTC_1884|Compound:5633444|FD-Score:-3.35|P-value:6.74E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.62|P-value:1.99E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.49|P-value:4.25E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.27|P-value:6.80E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.81|P-value:1.38E-6 ID:SGTC_2346|Compound:9031566|FD-Score:4.54|P-value:4.78E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.08|P-value:4.57E-5 ID:SGTC_2452|Compound:5863136|FD-Score:4.52|P-value:5.19E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.93|P-value:8.05E-12 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.19|P-value:8.95E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.15|P-value:1.75E-12 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.75|P-value:2.41E-14 ID:SGTC_2756|Compound:carbadox|FD-Score:3.26|P-value:7.07E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:4.41|P-value:8.36E-6 ID:SGTC_2886|Compound:9058944|FD-Score:3.71|P-value:1.45E-4 ID:SGTC_2891|Compound:9063876|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-9.37|P-value:8.56E-20 ID:SGTC_2935|Compound:9038016|FD-Score:6.48|P-value:1.45E-10 ID:SGTC_3017|Compound:9080681|FD-Score:3.76|P-value:1.21E-4

Top fitness defect scores for YEL003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_117 lucigenin 16.70 22.1 µM 3.57E-59 redox potentiating 2 7.94E-63
2 SGTC_116 0155-0182 9.07 31.8 µM 6.42E-19 mitochondrial response to ROS 7 6.10E-20
3 SGTC_2688 kanamycin b 7.75 96.7 µM 2.41E-14 calcium & mitochondrial duress 74 4.46E-15
4 SGTC_2619 obliquin 7.15 87.9 µM 1.75E-12 cell wall 56 4.24E-13
5 SGTC_2582 agaric acid 6.93 100.0 µM 8.05E-12 redox potentiating 131 2.14E-12
6 SGTC_2935 9038016 6.48 30.0 µM 1.45E-10 5 4.60E-11
7 SGTC_57 miconazole 5.61 162.5 nM 2.36E-8 ergosterol depletion effects on membrane 14 1.02E-8
8 SGTC_138 0850-0259 5.31 40.9 µM 1.13E-7 cell wall 133 5.34E-8
9 SGTC_1712 st032288 5.31 53.4 µM 1.14E-7 calcium & mitochondrial duress 103 5.41E-8
10 SGTC_1075 aripiprazole 5.12 15.2 µM 3.00E-7 8 1.51E-7
11 SGTC_2341 7987486 4.81 176.7 µM 1.38E-6 7 7.58E-7
12 SGTC_2346 9031566 4.54 143.8 µM 4.78E-6 Golgi 11 2.83E-6
13 SGTC_2452 5863136 4.52 48.4 µM 5.19E-6 13 3.08E-6
14 SGTC_1374 2889-5571 4.45 141.0 µM 6.97E-6 RSC complex & mRNA processing 26 4.22E-6
15 SGTC_2777 climbazole 4.41 1.5 µM 8.36E-6 iron homeostasis 33 5.11E-6
16 SGTC_141 0443-0274 4.33 32.3 µM 1.18E-5 mitochondrial response to ROS 32 7.35E-6
17 SGTC_902 1120-0002 4.30 2.1 µM 1.33E-5 azole & statin 39 8.37E-6
18 SGTC_467 ro 31-8220 4.03 72.8 µM 4.16E-5 calcium & mitochondrial duress 75 2.78E-5
19 SGTC_529 4076-0302 4.01 30.3 µM 4.57E-5 mitochondrial processes 19 3.08E-5
20 SGTC_333 3486-0131 3.92 40.1 µM 6.51E-5 amide catabolism 12 4.47E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.251 3.00E-49 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.218 2.34E-37 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.203 2.59E-32 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.198 8.57E-31 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.192 3.29E-29 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.191 5.88E-29 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.191 7.05E-29 YGR078C PAC10 hom Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
0.186 2.51E-27 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.179 1.39E-25 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.175 1.80E-24 YCR064C_d YCR064C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
0.174 3.48E-24 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.171 3.09E-23 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.169 7.35E-23 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.164 1.19E-21 YDR483W KRE2 hom Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
0.158 4.84E-20 YLR402W_d YLR402W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data