YEL015W / EDC3

Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL015W / EDC3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.26|P-value:2.22E-9 ID:SGTC_64|Compound:000l-0018|FD-Score:6.89|P-value:5.71E-11 ID:SGTC_315|Compound:k915-0449|FD-Score:-3.67|P-value:3.39E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.53|P-value:4.49E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.11|P-value:5.70E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:-5.42|P-value:2.53E-7 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:6.47E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.89|P-value:1.59E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-4.18|P-value:5.39E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.08|P-value:6.38E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:3.81|P-value:1.72E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.77|P-value:2.02E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.70|P-value:2.57E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.92|P-value:1.95E-6 ID:SGTC_1296|Compound:1068-0102|FD-Score:4.54|P-value:1.02E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.08|P-value:8.02E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.42|P-value:7.99E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.00|P-value:8.64E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.51|P-value:5.80E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:4.33|P-value:2.39E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.52|P-value:4.63E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.38|P-value:7.46E-4 ID:SGTC_1688|Compound:st021091|FD-Score:-3.50|P-value:5.98E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.63|P-value:3.99E-4 ID:SGTC_1811|Compound:st050069|FD-Score:3.59|P-value:3.65E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.87|P-value:1.74E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.75|P-value:4.15E-6 ID:SGTC_2157|Compound:5649910|FD-Score:3.44|P-value:6.02E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.98|P-value:1.17E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.41|P-value:6.75E-4 ID:SGTC_2269|Compound:7935389|FD-Score:3.50|P-value:5.00E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-5.18|P-value:7.94E-7 ID:SGTC_2464|Compound:5717415|FD-Score:3.33|P-value:8.59E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.97|P-value:1.20E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-4.10|P-value:7.43E-5 ID:SGTC_2707|Compound:st078556|FD-Score:3.82|P-value:1.64E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-4.82|P-value:3.99E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.45|P-value:1.46E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:-3.61|P-value:4.18E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.32|P-value:9.05E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.64|P-value:3.11E-4 ID:SGTC_3299|Compound:9121982|FD-Score:4.04|P-value:7.28E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:6.26|P-value:2.22E-9 ID:SGTC_64|Compound:000l-0018|FD-Score:6.89|P-value:5.71E-11 ID:SGTC_315|Compound:k915-0449|FD-Score:-3.67|P-value:3.39E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.53|P-value:4.49E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.11|P-value:5.70E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:-5.42|P-value:2.53E-7 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:6.47E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.89|P-value:1.59E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-4.18|P-value:5.39E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.08|P-value:6.38E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:3.81|P-value:1.72E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.77|P-value:2.02E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.70|P-value:2.57E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.92|P-value:1.95E-6 ID:SGTC_1296|Compound:1068-0102|FD-Score:4.54|P-value:1.02E-5 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.08|P-value:8.02E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.42|P-value:7.99E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.00|P-value:8.64E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.51|P-value:5.80E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:4.33|P-value:2.39E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.52|P-value:4.63E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.38|P-value:7.46E-4 ID:SGTC_1688|Compound:st021091|FD-Score:-3.50|P-value:5.98E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.63|P-value:3.99E-4 ID:SGTC_1811|Compound:st050069|FD-Score:3.59|P-value:3.65E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.87|P-value:1.74E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.75|P-value:4.15E-6 ID:SGTC_2157|Compound:5649910|FD-Score:3.44|P-value:6.02E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.98|P-value:1.17E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.41|P-value:6.75E-4 ID:SGTC_2269|Compound:7935389|FD-Score:3.50|P-value:5.00E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-5.18|P-value:7.94E-7 ID:SGTC_2464|Compound:5717415|FD-Score:3.33|P-value:8.59E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.97|P-value:1.20E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-4.10|P-value:7.43E-5 ID:SGTC_2707|Compound:st078556|FD-Score:3.82|P-value:1.64E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-4.82|P-value:3.99E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.45|P-value:1.46E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:-3.61|P-value:4.18E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.32|P-value:9.05E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.64|P-value:3.11E-4 ID:SGTC_3299|Compound:9121982|FD-Score:4.04|P-value:7.28E-5

Top fitness defect scores for YEL015W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_64 000l-0018 6.89 272.1 µM 5.71E-11 ubiquinone biosynthesis & proteasome 60 2.88E-12
2 SGTC_58 0957-0393 6.26 9.0 µM 2.22E-9 cell wall 198 1.88E-10
3 SGTC_1133 3473-0247 4.92 50.0 µM 1.95E-6 RSC complex & mRNA processing 24 4.29E-7
4 SGTC_2150 5556613 4.75 200.0 µM 4.15E-6 7 1.01E-6
5 SGTC_1296 1068-0102 4.54 11.9 µM 1.02E-5 7 2.80E-6
6 SGTC_2751 anetholtrithion 4.45 17.2 µM 1.46E-5 19 4.21E-6
7 SGTC_1377 2944-0135 4.33 198.0 µM 2.39E-5 calcium & mitochondrial duress 26 7.41E-6
8 SGTC_410 α-linolenic acid 4.11 100.0 µM 5.70E-5 excess fatty acid 34 1.99E-5
9 SGTC_608 k048-0125 4.08 159.0 µM 6.38E-5 26 2.26E-5
10 SGTC_3299 9121982 4.04 43.1 µM 7.28E-5 24 2.62E-5
11 SGTC_1351 1496-0003 4.00 46.5 µM 8.64E-5 22 3.18E-5
12 SGTC_2707 st078556 3.82 66.6 µM 1.64E-4 14 6.60E-5
13 SGTC_644 1498-1197 3.81 63.3 µM 1.72E-4 27 6.95E-5
14 SGTC_879 0831-1030 3.77 162.0 µM 2.02E-4 52 8.33E-5
15 SGTC_903 1134-0042 3.70 114.0 µM 2.57E-4 27 1.10E-4
16 SGTC_2791 5663188 3.64 71.4 µM 3.11E-4 RPP1 & pyrimidine depletion 29 1.36E-4
17 SGTC_1811 st050069 3.59 61.5 µM 3.65E-4 7 1.63E-4
18 SGTC_404 fumagillin 3.53 50.0 µM 4.49E-4 33 2.06E-4
19 SGTC_1529 apigenin 3.52 74.0 µM 4.63E-4 47 2.14E-4
20 SGTC_2269 7935389 3.50 174.4 µM 5.00E-4 tubulin folding & SWR complex 40 2.33E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 1.70E-20 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.155 2.49E-19 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.151 1.50E-18 YPL144W POC4 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.148 8.14E-18 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.141 1.95E-16 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.141 2.80E-16 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.137 1.43E-15 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.134 5.95E-15 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.131 2.57E-14 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.130 4.89E-14 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.128 9.29E-14 YNL016W PUB1 hom Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
0.124 4.75E-13 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.123 7.72E-13 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.117 9.43E-12 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.117 1.03E-11 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation