YEL060C / PRB1

Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL060C / PRB1

Click on Significant Values for Screen Details ID:SGTC_302|Compound:1094-0063|FD-Score:-2.79|P-value:4.46E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:2.69|P-value:8.13E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.03|P-value:1.57E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:2.91|P-value:3.34E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:2.65|P-value:9.79E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.88|P-value:2.61E-6 ID:SGTC_713|Compound:1488-1073|FD-Score:3.45|P-value:2.64E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:2.68|P-value:8.52E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.98|P-value:1.50E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.22|P-value:6.41E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:2.72|P-value:7.22E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.03|P-value:1.91E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-2.69|P-value:6.78E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.71|P-value:7.68E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.23|P-value:7.77E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.12|P-value:1.29E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:2.77|P-value:5.84E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:5.16|P-value:6.77E-10 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.99|P-value:1.09E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.09|P-value:5.93E-7 ID:SGTC_1508|Compound:1080-0625|FD-Score:2.86|P-value:4.05E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-2.60|P-value:9.84E-4 ID:SGTC_1716|Compound:st033144|FD-Score:2.86|P-value:4.03E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.33|P-value:3.90E-5 ID:SGTC_2115|Compound:tiliquinol|FD-Score:-3.01|P-value:1.74E-4 ID:SGTC_2117|Compound:5521686|FD-Score:-4.11|P-value:5.43E-7 ID:SGTC_2241|Compound:6772625|FD-Score:-3.73|P-value:4.72E-6 ID:SGTC_2293|Compound:7624732|FD-Score:-3.58|P-value:1.05E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.02|P-value:2.04E-4 ID:SGTC_2324|Compound:6149169|FD-Score:2.75|P-value:6.56E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-2.89|P-value:2.96E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.79|P-value:5.39E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:3.15|P-value:1.10E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:4.11|P-value:6.98E-7 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.24|P-value:7.44E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-2.96|P-value:2.13E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.32|P-value:5.07E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:6.36|P-value:3.80E-14 ID:SGTC_2601|Compound:securinine|FD-Score:-3.87|P-value:2.19E-6 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-7.22|P-value:6.32E-18 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.66|P-value:8.63E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-5.27|P-value:2.14E-10 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:2.77|P-value:5.87E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.02|P-value:7.60E-13 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.36|P-value:3.21E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-3.62|P-value:8.66E-6 ID:SGTC_2858|Compound:9025781|FD-Score:3.38|P-value:3.66E-5 ID:SGTC_2867|Compound:9039098|FD-Score:-2.88|P-value:3.10E-4 ID:SGTC_2899|Compound:9053889|FD-Score:2.66|P-value:9.12E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:2.78|P-value:5.72E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-2.77|P-value:4.90E-4 ID:SGTC_3169|Compound:9102970|FD-Score:-2.63|P-value:8.80E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-2.79|P-value:4.46E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:2.69|P-value:8.13E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.03|P-value:1.57E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:2.91|P-value:3.34E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:2.65|P-value:9.79E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.88|P-value:2.61E-6 ID:SGTC_713|Compound:1488-1073|FD-Score:3.45|P-value:2.64E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:2.68|P-value:8.52E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.98|P-value:1.50E-6 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.22|P-value:6.41E-5 ID:SGTC_876|Compound:0720-0963|FD-Score:2.72|P-value:7.22E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.03|P-value:1.91E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-2.69|P-value:6.78E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.71|P-value:7.68E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.23|P-value:7.77E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.12|P-value:1.29E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:2.77|P-value:5.84E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:5.16|P-value:6.77E-10 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.99|P-value:1.09E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.09|P-value:5.93E-7 ID:SGTC_1508|Compound:1080-0625|FD-Score:2.86|P-value:4.05E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-2.60|P-value:9.84E-4 ID:SGTC_1716|Compound:st033144|FD-Score:2.86|P-value:4.03E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.33|P-value:3.90E-5 ID:SGTC_2115|Compound:tiliquinol|FD-Score:-3.01|P-value:1.74E-4 ID:SGTC_2117|Compound:5521686|FD-Score:-4.11|P-value:5.43E-7 ID:SGTC_2241|Compound:6772625|FD-Score:-3.73|P-value:4.72E-6 ID:SGTC_2293|Compound:7624732|FD-Score:-3.58|P-value:1.05E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.02|P-value:2.04E-4 ID:SGTC_2324|Compound:6149169|FD-Score:2.75|P-value:6.56E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-2.89|P-value:2.96E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.79|P-value:5.39E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:3.15|P-value:1.10E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:4.11|P-value:6.98E-7 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.24|P-value:7.44E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-2.96|P-value:2.13E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.32|P-value:5.07E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:6.36|P-value:3.80E-14 ID:SGTC_2601|Compound:securinine|FD-Score:-3.87|P-value:2.19E-6 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-7.22|P-value:6.32E-18 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.66|P-value:8.63E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-5.27|P-value:2.14E-10 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:2.77|P-value:5.87E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.02|P-value:7.60E-13 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.36|P-value:3.21E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-3.62|P-value:8.66E-6 ID:SGTC_2858|Compound:9025781|FD-Score:3.38|P-value:3.66E-5 ID:SGTC_2867|Compound:9039098|FD-Score:-2.88|P-value:3.10E-4 ID:SGTC_2899|Compound:9053889|FD-Score:2.66|P-value:9.12E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:2.78|P-value:5.72E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-2.77|P-value:4.90E-4 ID:SGTC_3169|Compound:9102970|FD-Score:-2.63|P-value:8.80E-4

Top fitness defect scores for YEL060C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2597 laccaic acid a 6.36 100.0 µM 3.80E-14 6 1.02E-10
2 SGTC_2752 diclazuril 6.02 33.1 µM 7.60E-13 56 8.96E-10
3 SGTC_1451 4152-0210 5.16 104.0 µM 6.77E-10 6 1.25E-7
4 SGTC_2491 paeonol 4.11 100.0 µM 6.98E-7 9 1.97E-5
5 SGTC_769 0302-0231 3.98 76.0 µM 1.50E-6 40 3.45E-5
6 SGTC_699 0375-0592 3.88 111.0 µM 2.61E-6 iron homeostasis 22 5.16E-5
7 SGTC_2621 lithochol-11-enic acid 3.66 100.0 µM 8.63E-6 35 1.24E-4
8 SGTC_713 1488-1073 3.45 324.0 µM 2.64E-5 ERG2 21 2.82E-4
9 SGTC_2858 9025781 3.38 9.0 µM 3.66E-5 sphingolipid biosynthesis & PDR1 134 3.59E-4
10 SGTC_2594 a-mangostin 3.32 100.0 µM 5.07E-5 57 4.56E-4
11 SGTC_2493 resveratrol 3.24 26.1 µM 7.44E-5 24 6.05E-4
12 SGTC_1214 0358-0031 3.23 63.7 µM 7.77E-5 41 6.24E-4
13 SGTC_2490 carminic acid 3.15 95.4 µM 1.10E-4 19 8.06E-4
14 SGTC_1247 0448-0043 3.12 5.8 µM 1.29E-4 ERG2 101 9.06E-4
15 SGTC_1139 k015-0024 3.03 46.9 µM 1.91E-4 PDR1 36 0.00121
16 SGTC_2297 7971671 3.02 152.3 µM 2.04E-4 RNA processing & uracil transport 62 0.00127
17 SGTC_549 1661-1316 2.91 519.0 µM 3.34E-4 64 0.00183
18 SGTC_1716 st033144 2.86 30.6 µM 4.03E-4 66 0.00211
19 SGTC_1508 1080-0625 2.86 72.9 µM 4.05E-4 54 0.00211
20 SGTC_2483 5729050 2.79 27.2 µM 5.39E-4 93 0.00261

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.235 3.20E-43 YMR322C_p SNO4_p hom Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
0.218 1.77E-37 YDR107C TMN2 hom Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
0.217 4.37E-37 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.206 1.27E-33 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.204 8.14E-33 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.199 2.00E-31 YPR159C-A_p YPR159C-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.190 1.24E-28 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.188 4.76E-28 YOR161C-C_p YOR161C-C_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.183 8.57E-27 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.181 3.09E-26 YLR213C CRR1 hom Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
0.181 3.95E-26 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C
0.178 2.53E-25 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.176 8.67E-25 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.172 9.30E-24 YOR301W RAX1 hom Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.172 1.44E-23 YDR517W GRH1 hom Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress