YEL071W / DLD3

D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL071W / DLD3

Click on Significant Values for Screen Details ID:SGTC_89|Compound:0302-0250|FD-Score:-3.12|P-value:6.82E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.37|P-value:2.71E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.36|P-value:3.86E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:3.98|P-value:2.22E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.54|P-value:1.42E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.44|P-value:2.10E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.15|P-value:6.24E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.96|P-value:2.36E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.07|P-value:8.30E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:-3.92|P-value:2.92E-5 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.23|P-value:4.54E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.08|P-value:8.04E-4 ID:SGTC_1970|Compound:st070122|FD-Score:3.02|P-value:9.72E-4 ID:SGTC_2094|Compound:5332512|FD-Score:5.14|P-value:6.77E-8 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.19|P-value:5.36E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.05|P-value:8.77E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.72|P-value:6.77E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.17|P-value:5.82E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.87|P-value:3.61E-5 ID:SGTC_2486|Compound:5283202|FD-Score:-3.78|P-value:5.41E-5 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.45|P-value:2.00E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.05|P-value:8.56E-4 ID:SGTC_2716|Compound:st080858|FD-Score:4.22|P-value:7.52E-6 ID:SGTC_3226|Compound:9131256|FD-Score:3.07|P-value:8.16E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.86|P-value:2.98E-7 ID:SGTC_3331|Compound:9141060|FD-Score:-3.15|P-value:6.20E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:-3.12|P-value:6.82E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.37|P-value:2.71E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.36|P-value:3.86E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:3.98|P-value:2.22E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.54|P-value:1.42E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.44|P-value:2.10E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.15|P-value:6.24E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.96|P-value:2.36E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.07|P-value:8.30E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:-3.92|P-value:2.92E-5 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.23|P-value:4.54E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.08|P-value:8.04E-4 ID:SGTC_1970|Compound:st070122|FD-Score:3.02|P-value:9.72E-4 ID:SGTC_2094|Compound:5332512|FD-Score:5.14|P-value:6.77E-8 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.19|P-value:5.36E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.05|P-value:8.77E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.72|P-value:6.77E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.17|P-value:5.82E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.87|P-value:3.61E-5 ID:SGTC_2486|Compound:5283202|FD-Score:-3.78|P-value:5.41E-5 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.45|P-value:2.00E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.05|P-value:8.56E-4 ID:SGTC_2716|Compound:st080858|FD-Score:4.22|P-value:7.52E-6 ID:SGTC_3226|Compound:9131256|FD-Score:3.07|P-value:8.16E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.86|P-value:2.98E-7 ID:SGTC_3331|Compound:9141060|FD-Score:-3.15|P-value:6.20E-4

Top fitness defect scores for YEL071W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2094 5332512 5.14 191.7 µM 6.77E-8 8 1.40E-7
2 SGTC_3323 9138564 4.86 53.9 µM 2.98E-7 8 5.73E-7
3 SGTC_2716 st080858 4.22 38.6 µM 7.52E-6 60S ribosome export 14 1.24E-5
4 SGTC_142 0335-0849 3.98 62.7 µM 2.22E-5 ubiquinone biosynthesis & proteasome 125 3.47E-5
5 SGTC_1251 0490-5699 3.96 265.0 µM 2.36E-5 33 3.68E-5
6 SGTC_2485 5633444 3.87 17.8 µM 3.61E-5 redox potentiating 47 5.52E-5
7 SGTC_2308 7584454 3.72 197.6 µM 6.77E-5 26 1.00E-4
8 SGTC_305 1682-2595 3.44 20.8 µM 2.10E-4 RNA processing & uracil transport 37 2.95E-4
9 SGTC_134 0269-0018 3.37 6.9 µM 2.71E-4 169 3.76E-4
10 SGTC_216 k038-0035 3.24 28.4 µM 4.37E-4 ubiquinone biosynthesis & proteasome 71 5.93E-4
11 SGTC_1506 4226-2149 3.23 212.0 µM 4.54E-4 64 6.15E-4
12 SGTC_2130 NSC 7 3.19 2.5 µM 5.36E-4 copper-dependent oxidative stress 73 7.20E-4
13 SGTC_3226 9131256 3.07 49.5 µM 8.16E-4 7 0.00108
14 SGTC_2708 st078559 3.05 56.0 µM 8.56E-4 28 0.00113
15 SGTC_2186 6124449 3.05 200.0 µM 8.77E-4 RSC complex & mRNA processing 56 0.00115
16 SGTC_1970 st070122 3.02 35.8 µM 9.72E-4 TSC3-RPN4 72 0.00127
17 SGTC_2478 5763493 2.95 32.3 µM 0.00122 64 0.00158
18 SGTC_916 4466-0038 2.93 42.0 nM 0.00130 cell wall 154 0.00167
19 SGTC_2093 5331342 2.93 194.4 µM 0.00133 61 0.00172
20 SGTC_545 4218-0007 2.83 221.0 µM 0.00183 73 0.00232

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 9.05E-20 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.124 6.83E-13 YOL034W SMC5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
0.123 8.20E-13 YPL277C_p YPL277C_p hom Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels
0.118 6.62E-12 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.117 8.81E-12 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.117 1.21E-11 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.116 1.44E-11 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.115 2.67E-11 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.108 2.93E-10 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.108 3.16E-10 YPR065W ROX1 hom Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.108 3.46E-10 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.107 4.41E-10 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.107 5.29E-10 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.107 5.23E-10 YJL087C TRL1 het tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
0.106 8.79E-10 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2