YER018C / SPC25

Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER018C / SPC25

Click on Significant Values for Screen Details ID:SGTC_159|Compound:3970-0959|FD-Score:3.56|P-value:5.97E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.18|P-value:3.89E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:2.88|P-value:8.88E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.93|P-value:9.96E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:4.04|P-value:6.45E-6 ID:SGTC_876|Compound:0720-0963|FD-Score:3.59|P-value:5.08E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.00|P-value:7.60E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.08|P-value:4.27E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:2.93|P-value:7.55E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.75|P-value:2.54E-5 ID:SGTC_1174|Compound:0739-0097|FD-Score:2.90|P-value:8.23E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-4.49|P-value:9.19E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.11|P-value:4.44E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:-4.09|P-value:7.33E-6 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.96|P-value:4.54E-8 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.49|P-value:7.91E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:2.97|P-value:6.36E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-4.43|P-value:1.30E-6 ID:SGTC_1629|Compound:st005085|FD-Score:-3.63|P-value:5.91E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.00|P-value:7.91E-6 ID:SGTC_1637|Compound:st007924|FD-Score:4.30|P-value:1.68E-6 ID:SGTC_1664|Compound:st014217|FD-Score:3.23|P-value:2.31E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.67|P-value:5.01E-5 ID:SGTC_1927|Compound:st060202|FD-Score:2.87|P-value:9.40E-4 ID:SGTC_1936|Compound:st074407|FD-Score:-3.57|P-value:7.93E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-2.98|P-value:8.19E-4 ID:SGTC_2011|Compound:4012611|FD-Score:5.32|P-value:4.80E-9 ID:SGTC_2182|Compound:6325192|FD-Score:3.45|P-value:9.28E-5 ID:SGTC_2186|Compound:6124449|FD-Score:3.14|P-value:3.41E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.85|P-value:1.56E-5 ID:SGTC_2458|Compound:5808907|FD-Score:-3.61|P-value:6.49E-5 ID:SGTC_2473|Compound:5854593|FD-Score:3.76|P-value:2.45E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.23|P-value:2.33E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.19|P-value:3.63E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.15|P-value:4.24E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-4.63|P-value:4.49E-7 ID:SGTC_2917|Compound:7979103|FD-Score:-4.57|P-value:6.22E-7 ID:SGTC_2928|Compound:9011484|FD-Score:-5.34|P-value:6.99E-9 ID:SGTC_2957|Compound:9076800|FD-Score:-3.03|P-value:6.82E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-8.84|P-value:2.30E-21 ID:SGTC_3253|Compound:9135987|FD-Score:4.74|P-value:1.62E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:3.56|P-value:5.97E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.18|P-value:3.89E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:2.88|P-value:8.88E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.93|P-value:9.96E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:4.04|P-value:6.45E-6 ID:SGTC_876|Compound:0720-0963|FD-Score:3.59|P-value:5.08E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.00|P-value:7.60E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.08|P-value:4.27E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:2.93|P-value:7.55E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.75|P-value:2.54E-5 ID:SGTC_1174|Compound:0739-0097|FD-Score:2.90|P-value:8.23E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-4.49|P-value:9.19E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.11|P-value:4.44E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:-4.09|P-value:7.33E-6 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.96|P-value:4.54E-8 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.49|P-value:7.91E-5 ID:SGTC_1527|Compound:coumarin|FD-Score:2.97|P-value:6.36E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-4.43|P-value:1.30E-6 ID:SGTC_1629|Compound:st005085|FD-Score:-3.63|P-value:5.91E-5 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.00|P-value:7.91E-6 ID:SGTC_1637|Compound:st007924|FD-Score:4.30|P-value:1.68E-6 ID:SGTC_1664|Compound:st014217|FD-Score:3.23|P-value:2.31E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.67|P-value:5.01E-5 ID:SGTC_1927|Compound:st060202|FD-Score:2.87|P-value:9.40E-4 ID:SGTC_1936|Compound:st074407|FD-Score:-3.57|P-value:7.93E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-2.98|P-value:8.19E-4 ID:SGTC_2011|Compound:4012611|FD-Score:5.32|P-value:4.80E-9 ID:SGTC_2182|Compound:6325192|FD-Score:3.45|P-value:9.28E-5 ID:SGTC_2186|Compound:6124449|FD-Score:3.14|P-value:3.41E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.85|P-value:1.56E-5 ID:SGTC_2458|Compound:5808907|FD-Score:-3.61|P-value:6.49E-5 ID:SGTC_2473|Compound:5854593|FD-Score:3.76|P-value:2.45E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.23|P-value:2.33E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.19|P-value:3.63E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.15|P-value:4.24E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-4.63|P-value:4.49E-7 ID:SGTC_2917|Compound:7979103|FD-Score:-4.57|P-value:6.22E-7 ID:SGTC_2928|Compound:9011484|FD-Score:-5.34|P-value:6.99E-9 ID:SGTC_2957|Compound:9076800|FD-Score:-3.03|P-value:6.82E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-8.84|P-value:2.30E-21 ID:SGTC_3253|Compound:9135987|FD-Score:4.74|P-value:1.62E-7

Top fitness defect scores for YER018C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2011 4012611 5.32 177.0 µM 4.80E-9 mitochondrial processes 4 5.12E-8 0.30
2 SGTC_1255 0566-0045 4.96 221.0 µM 4.54E-8 excess fatty acid 10 3.62E-7 0.09
3 SGTC_3253 9135987 4.74 49.5 µM 1.62E-7 60S ribosome export 2 1.09E-6 0.65
4 SGTC_1637 st007924 4.30 17.4 µM 1.68E-6 3 8.39E-6 0.36
5 SGTC_1246 0422-0085 4.11 105.0 µM 4.44E-6 10 1.96E-5 0.33
6 SGTC_795 0685-0265 4.04 78.0 µM 6.45E-6 excess fatty acid 16 2.70E-5 0.01
7 SGTC_1631 benzylvanillin 4.00 81.9 µM 7.91E-6 Golgi 6 3.23E-5 0.17
8 SGTC_2349 9023730 3.85 187.1 µM 1.56E-5 5 5.84E-5 0.36
9 SGTC_2473 5854593 3.76 8.9 µM 2.45E-5 3 8.65E-5 0.06
10 SGTC_1148 3909-7663 3.75 27.7 µM 2.54E-5 3 8.93E-5 0.11
11 SGTC_876 0720-0963 3.59 56.8 µM 5.08E-5 9 1.63E-4 0.05
12 SGTC_159 3970-0959 3.56 50.0 µM 5.97E-5 24 1.88E-4 0.05
13 SGTC_1264 0783-0040 3.49 158.0 µM 7.91E-5 6 2.40E-4 0.29
14 SGTC_2182 6325192 3.45 200.0 µM 9.28E-5 Golgi 3 2.76E-4 0.33
15 SGTC_1664 st014217 3.23 9.0 µM 2.31E-4 60S ribosome export 12 6.10E-4 0.08
16 SGTC_2541 muurolladie-3-one 3.23 83.1 µM 2.33E-4 excess fatty acid 29 6.16E-4 0.06
17 SGTC_2186 6124449 3.14 200.0 µM 3.41E-4 RSC complex & mRNA processing 29 8.56E-4 0.02
18 SGTC_951 1171-0579 3.08 82.7 µM 4.27E-4 cell wall 39 0.00104 0.15
19 SGTC_1527 coumarin 2.97 137.0 µM 6.36E-4 16 0.00148 0.04
20 SGTC_1003 4049-0218 2.93 100.0 µM 7.55E-4 29 0.00171 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 2.98E-21 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.149 4.79E-18 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.146 2.34E-17 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.135 3.36E-15 YDR479C PEX29 hom Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
0.123 7.48E-13 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.120 2.77E-12 YGL142C GPI10 het Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
0.119 4.51E-12 YMR288W HSH155 het U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
0.119 5.56E-12 YER147C SCC4 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
0.118 8.03E-12 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.115 2.03E-11 YDR373W FRQ1 het N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
0.115 2.82E-11 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.112 8.29E-11 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.109 2.32E-10 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.106 9.07E-10 YGR198W YPP1 het Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
0.104 1.58E-9 YDR082W STN1 het Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping