YER021W / RPN3

Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER021W / RPN3

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:3.75|P-value:5.26E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:-3.63|P-value:9.47E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.72|P-value:1.98E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:-4.11|P-value:2.14E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-4.67|P-value:3.01E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.88|P-value:4.42E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.99|P-value:3.15E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-4.45|P-value:6.59E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:5.28|P-value:2.10E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:3.99|P-value:2.51E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:5.31|P-value:1.89E-6 ID:SGTC_634|Compound:1611-4317|FD-Score:5.54|P-value:7.20E-7 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.83|P-value:5.17E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:3.99|P-value:2.49E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:4.65|P-value:2.53E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.71|P-value:5.92E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:4.02|P-value:2.30E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:4.27|P-value:9.95E-5 ID:SGTC_1009|Compound:4106-0057|FD-Score:-3.62|P-value:9.61E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:9.13|P-value:1.15E-15 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.00|P-value:3.00E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.67|P-value:6.81E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:7.46|P-value:4.51E-11 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.59|P-value:8.64E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:8.73|P-value:1.66E-14 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.79|P-value:4.76E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.14|P-value:1.53E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.06|P-value:9.31E-8 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.55|P-value:9.66E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:-4.00|P-value:3.04E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.79|P-value:5.83E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:6.15|P-value:4.38E-8 ID:SGTC_1453|Compound:4237-0541|FD-Score:9.22|P-value:6.08E-16 ID:SGTC_1455|Compound:4262-0120|FD-Score:8.63|P-value:3.27E-14 ID:SGTC_1461|Compound:k072-0203|FD-Score:8.13|P-value:7.96E-13 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:4.65|P-value:2.56E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.21|P-value:1.21E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.54|P-value:9.82E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.61|P-value:9.88E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.65|P-value:2.50E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.63|P-value:7.64E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.71|P-value:2.65E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.12|P-value:1.61E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-4.19|P-value:1.63E-4 ID:SGTC_1993|Compound:st073995|FD-Score:4.64|P-value:2.63E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.68|P-value:6.63E-4 ID:SGTC_2044|Compound:5102272|FD-Score:5.12|P-value:4.16E-6 ID:SGTC_2046|Compound:4110841|FD-Score:3.66|P-value:7.06E-4 ID:SGTC_2083|Compound:5306046|FD-Score:4.09|P-value:1.82E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.65|P-value:7.25E-4 ID:SGTC_2112|Compound:5488546|FD-Score:4.02|P-value:2.29E-4 ID:SGTC_2124|Compound:5246320|FD-Score:13.90|P-value:6.91E-34 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.12|P-value:1.64E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.44|P-value:5.40E-5 ID:SGTC_2256|Compound:7954252|FD-Score:3.79|P-value:4.67E-4 ID:SGTC_2269|Compound:7935389|FD-Score:4.30|P-value:8.98E-5 ID:SGTC_2275|Compound:7947463|FD-Score:5.79|P-value:2.33E-7 ID:SGTC_2289|Compound:7946404|FD-Score:6.05|P-value:7.12E-8 ID:SGTC_2336|Compound:9001819|FD-Score:17.80|P-value:8.04E-54 ID:SGTC_2370|Compound:9071495|FD-Score:4.74|P-value:1.83E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:6.11|P-value:5.39E-8 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.85|P-value:3.89E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.99|P-value:2.47E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.89|P-value:3.46E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.83|P-value:4.13E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.63|P-value:3.53E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.59|P-value:8.46E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.70|P-value:6.14E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:3.89|P-value:3.39E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.99|P-value:2.53E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.90|P-value:3.35E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.37|P-value:8.81E-5 ID:SGTC_2790|Compound:5423970|FD-Score:5.99|P-value:9.39E-8 ID:SGTC_2921|Compound:7974240|FD-Score:3.57|P-value:9.23E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.56|P-value:9.49E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.73|P-value:6.94E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.92|P-value:1.17E-5 ID:SGTC_3142|Compound:9086498|FD-Score:5.43|P-value:1.12E-6 ID:SGTC_3327|Compound:9140648|FD-Score:5.18|P-value:3.19E-6 ID:SGTC_3346|Compound:9158254|FD-Score:3.77|P-value:5.03E-4 ID:SGTC_3352|Compound:9151559|FD-Score:3.65|P-value:7.17E-4 ID:SGTC_3356|Compound:9151811|FD-Score:5.10|P-value:4.45E-6 ID:SGTC_1|Compound:0258-0098|FD-Score:3.75|P-value:5.26E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:-3.63|P-value:9.47E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.72|P-value:1.98E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:-4.11|P-value:2.14E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-4.67|P-value:3.01E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.88|P-value:4.42E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.99|P-value:3.15E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-4.45|P-value:6.59E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:5.28|P-value:2.10E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:3.99|P-value:2.51E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:5.31|P-value:1.89E-6 ID:SGTC_634|Compound:1611-4317|FD-Score:5.54|P-value:7.20E-7 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.83|P-value:5.17E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:3.99|P-value:2.49E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:4.65|P-value:2.53E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.71|P-value:5.92E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:4.02|P-value:2.30E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:4.27|P-value:9.95E-5 ID:SGTC_1009|Compound:4106-0057|FD-Score:-3.62|P-value:9.61E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:9.13|P-value:1.15E-15 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.00|P-value:3.00E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.67|P-value:6.81E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:7.46|P-value:4.51E-11 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.59|P-value:8.64E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:8.73|P-value:1.66E-14 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.79|P-value:4.76E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.14|P-value:1.53E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.06|P-value:9.31E-8 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.55|P-value:9.66E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:-4.00|P-value:3.04E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.79|P-value:5.83E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:6.15|P-value:4.38E-8 ID:SGTC_1453|Compound:4237-0541|FD-Score:9.22|P-value:6.08E-16 ID:SGTC_1455|Compound:4262-0120|FD-Score:8.63|P-value:3.27E-14 ID:SGTC_1461|Compound:k072-0203|FD-Score:8.13|P-value:7.96E-13 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:4.65|P-value:2.56E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.21|P-value:1.21E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.54|P-value:9.82E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.61|P-value:9.88E-4 ID:SGTC_1727|Compound:st034309|FD-Score:4.65|P-value:2.50E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.63|P-value:7.64E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.71|P-value:2.65E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.12|P-value:1.61E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-4.19|P-value:1.63E-4 ID:SGTC_1993|Compound:st073995|FD-Score:4.64|P-value:2.63E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.68|P-value:6.63E-4 ID:SGTC_2044|Compound:5102272|FD-Score:5.12|P-value:4.16E-6 ID:SGTC_2046|Compound:4110841|FD-Score:3.66|P-value:7.06E-4 ID:SGTC_2083|Compound:5306046|FD-Score:4.09|P-value:1.82E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.65|P-value:7.25E-4 ID:SGTC_2112|Compound:5488546|FD-Score:4.02|P-value:2.29E-4 ID:SGTC_2124|Compound:5246320|FD-Score:13.90|P-value:6.91E-34 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.12|P-value:1.64E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.44|P-value:5.40E-5 ID:SGTC_2256|Compound:7954252|FD-Score:3.79|P-value:4.67E-4 ID:SGTC_2269|Compound:7935389|FD-Score:4.30|P-value:8.98E-5 ID:SGTC_2275|Compound:7947463|FD-Score:5.79|P-value:2.33E-7 ID:SGTC_2289|Compound:7946404|FD-Score:6.05|P-value:7.12E-8 ID:SGTC_2336|Compound:9001819|FD-Score:17.80|P-value:8.04E-54 ID:SGTC_2370|Compound:9071495|FD-Score:4.74|P-value:1.83E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:6.11|P-value:5.39E-8 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.85|P-value:3.89E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.99|P-value:2.47E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.89|P-value:3.46E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.83|P-value:4.13E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.63|P-value:3.53E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.59|P-value:8.46E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.70|P-value:6.14E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:3.89|P-value:3.39E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.99|P-value:2.53E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:3.90|P-value:3.35E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.37|P-value:8.81E-5 ID:SGTC_2790|Compound:5423970|FD-Score:5.99|P-value:9.39E-8 ID:SGTC_2921|Compound:7974240|FD-Score:3.57|P-value:9.23E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.56|P-value:9.49E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.73|P-value:6.94E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.92|P-value:1.17E-5 ID:SGTC_3142|Compound:9086498|FD-Score:5.43|P-value:1.12E-6 ID:SGTC_3327|Compound:9140648|FD-Score:5.18|P-value:3.19E-6 ID:SGTC_3346|Compound:9158254|FD-Score:3.77|P-value:5.03E-4 ID:SGTC_3352|Compound:9151559|FD-Score:3.65|P-value:7.17E-4 ID:SGTC_3356|Compound:9151811|FD-Score:5.10|P-value:4.45E-6

Top fitness defect scores for YER021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2336 9001819 17.80 198.7 µM 8.04E-54 heme biosynthesis & mitochondrial translocase 2 6.09E-71 4.29
2 SGTC_2124 5246320 13.90 200.0 µM 6.91E-34 heme biosynthesis & mitochondrial translocase 4 2.15E-44 2.35
3 SGTC_1453 4237-0541 9.22 195.0 µM 6.08E-16 heme biosynthesis & mitochondrial translocase 3 1.53E-20 2.26
4 SGTC_1016 4226-1401 9.13 38.0 µM 1.15E-15 RNA pol III & RNase P/MRP 4 3.58E-20 1.77
5 SGTC_1113 artemisinin 8.73 1.2 µM 1.66E-14 heme biosynthesis & mitochondrial translocase 6 1.23E-18 0.55
6 SGTC_1455 4262-0120 8.63 131.0 µM 3.27E-14 plasma membrane duress 1 3.02E-18 3.38
7 SGTC_1461 k072-0203 8.13 53.8 µM 7.96E-13 1 2.07E-16 3.06
8 SGTC_1098 clofazimine 7.46 15.1 µM 4.51E-11 heme biosynthesis & mitochondrial translocase 7 4.34E-14 0.59
9 SGTC_1446 4239-0190 6.15 62.0 µM 4.38E-8 2 3.85E-10 0.92
10 SGTC_2519 cryptotanshinone 6.11 88.0 µM 5.39E-8 heme biosynthesis & mitochondrial translocase 7 5.07E-10 0.12
11 SGTC_2289 7946404 6.05 165.1 µM 7.12E-8 6 7.30E-10 0.42
12 SGTC_2790 5423970 5.99 71.4 µM 9.39E-8 endomembrane recycling 5 1.05E-9 0.24
13 SGTC_2275 7947463 5.79 200.0 µM 2.33E-7 iron homeostasis 3 3.49E-9 0.45
14 SGTC_634 1611-4317 5.54 11.2 µM 7.20E-7 ERG2 1 1.54E-8 0.46
15 SGTC_3142 9086498 5.43 49.5 µM 1.12E-6 iron homeostasis 28 2.74E-8 0.31
16 SGTC_623 k072-0108 5.31 16.9 µM 1.89E-6 heme biosynthesis & mitochondrial translocase 9 5.47E-8 0.22
17 SGTC_553 k007-0256 5.28 45.7 µM 2.10E-6 heme biosynthesis & mitochondrial translocase 13 6.32E-8 0.14
18 SGTC_3327 9140648 5.18 26.9 µM 3.19E-6 6 1.09E-7 0.32
19 SGTC_2044 5102272 5.12 200.0 µM 4.16E-6 2 1.55E-7 0.37
20 SGTC_3356 9151811 5.10 4.5 µM 4.45E-6 cell wall 2 1.69E-7 0.20

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.389 8.62E-122 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.380 1.62E-115 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.355 4.18E-100 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.345 1.07E-94 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.319 2.31E-80 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.268 2.87E-56 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.264 1.57E-54 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.254 2.26E-50 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.244 9.36E-47 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.236 1.29E-43 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.220 6.80E-38 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.209 1.68E-34 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.204 6.83E-33 YIL075C RPN2 het Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
0.199 2.01E-31 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.195 3.27E-30 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication