YER049W / TPA1

Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate

Zygosity: Homozygous strain
fixedexpanded
Profile for YER049W / TPA1

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:2.94|P-value:4.63E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.50|P-value:3.16E-5 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.84|P-value:5.83E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-2.71|P-value:9.67E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.70|P-value:1.55E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.32|P-value:7.47E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.84|P-value:5.79E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:5.54|P-value:1.99E-10 ID:SGTC_1270|Compound:0831-1170|FD-Score:4.42|P-value:3.10E-7 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.29|P-value:1.05E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-3.39|P-value:5.36E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-2.81|P-value:6.53E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.98|P-value:2.76E-6 ID:SGTC_1527|Compound:coumarin|FD-Score:2.76|P-value:9.47E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-2.99|P-value:3.14E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.34|P-value:8.57E-5 ID:SGTC_1784|Compound:st039334|FD-Score:4.20|P-value:1.08E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.74|P-value:9.69E-6 ID:SGTC_2103|Compound:5378138|FD-Score:2.84|P-value:7.17E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-2.97|P-value:3.35E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-2.77|P-value:7.47E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-2.70|P-value:9.94E-4 ID:SGTC_2545|Compound:solidagenone|FD-Score:2.82|P-value:7.67E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:3.46|P-value:4.89E-5 ID:SGTC_2551|Compound:apiole|FD-Score:2.98|P-value:3.93E-4 ID:SGTC_2827|Compound:7993847|FD-Score:2.97|P-value:4.22E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.76|P-value:1.12E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.74|P-value:1.24E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.40|P-value:6.48E-5 ID:SGTC_2941|Compound:9056145|FD-Score:2.82|P-value:7.52E-4 ID:SGTC_2945|Compound:9067681|FD-Score:5.20|P-value:2.13E-9 ID:SGTC_2948|Compound:9070189|FD-Score:3.10|P-value:2.40E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.84|P-value:6.92E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.38|P-value:7.10E-5 ID:SGTC_3294|Compound:9122953|FD-Score:2.83|P-value:7.29E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.12|P-value:2.23E-4 ID:SGTC_3332|Compound:9141069|FD-Score:2.79|P-value:8.44E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-5.59|P-value:9.21E-11 ID:SGTC_3339|Compound:9145883|FD-Score:3.14|P-value:2.09E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:2.94|P-value:4.63E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.50|P-value:3.16E-5 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.84|P-value:5.83E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-2.71|P-value:9.67E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.70|P-value:1.55E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.32|P-value:7.47E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.84|P-value:5.79E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:5.54|P-value:1.99E-10 ID:SGTC_1270|Compound:0831-1170|FD-Score:4.42|P-value:3.10E-7 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.29|P-value:1.05E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-3.39|P-value:5.36E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-2.81|P-value:6.53E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.98|P-value:2.76E-6 ID:SGTC_1527|Compound:coumarin|FD-Score:2.76|P-value:9.47E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-2.99|P-value:3.14E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.34|P-value:8.57E-5 ID:SGTC_1784|Compound:st039334|FD-Score:4.20|P-value:1.08E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.74|P-value:9.69E-6 ID:SGTC_2103|Compound:5378138|FD-Score:2.84|P-value:7.17E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-2.97|P-value:3.35E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-2.77|P-value:7.47E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-2.70|P-value:9.94E-4 ID:SGTC_2545|Compound:solidagenone|FD-Score:2.82|P-value:7.67E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:3.46|P-value:4.89E-5 ID:SGTC_2551|Compound:apiole|FD-Score:2.98|P-value:3.93E-4 ID:SGTC_2827|Compound:7993847|FD-Score:2.97|P-value:4.22E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.76|P-value:1.12E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.74|P-value:1.24E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.40|P-value:6.48E-5 ID:SGTC_2941|Compound:9056145|FD-Score:2.82|P-value:7.52E-4 ID:SGTC_2945|Compound:9067681|FD-Score:5.20|P-value:2.13E-9 ID:SGTC_2948|Compound:9070189|FD-Score:3.10|P-value:2.40E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.84|P-value:6.92E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.38|P-value:7.10E-5 ID:SGTC_3294|Compound:9122953|FD-Score:2.83|P-value:7.29E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.12|P-value:2.23E-4 ID:SGTC_3332|Compound:9141069|FD-Score:2.79|P-value:8.44E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-5.59|P-value:9.21E-11 ID:SGTC_3339|Compound:9145883|FD-Score:3.14|P-value:2.09E-4

Top fitness defect scores for YER049W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1206 3978-0017 5.54 132.0 µM 1.99E-10 ERG2 11 1.53E-8
2 SGTC_2945 9067681 5.20 61.8 µM 2.13E-9 6 9.83E-8
3 SGTC_1270 0831-1170 4.42 17.3 µM 3.10E-7 6 4.97E-6
4 SGTC_1784 st039334 4.20 36.3 µM 1.08E-6 15 1.33E-5
5 SGTC_2837 9008476 3.76 45.5 µM 1.12E-5 41 8.42E-5
6 SGTC_2934 9036370 3.74 36.9 µM 1.24E-5 39 9.10E-5
7 SGTC_676 falcarinol 3.70 140.0 µM 1.55E-5 62 1.09E-4
8 SGTC_2550 chrysin dimethyl ether 3.46 71.9 µM 4.89E-5 15 2.71E-4
9 SGTC_2935 9038016 3.40 30.0 µM 6.48E-5 33 3.38E-4
10 SGTC_3072 9116419 3.38 49.5 µM 7.10E-5 ERG2 39 3.64E-4
11 SGTC_1739 st037196 3.34 92.5 µM 8.57E-5 44 4.23E-4
12 SGTC_1296 1068-0102 3.29 11.9 µM 1.05E-4 21 4.96E-4
13 SGTC_3339 9145883 3.14 14.9 µM 2.09E-4 42 8.57E-4
14 SGTC_3319 9138450 3.12 16.8 µM 2.23E-4 44 9.04E-4
15 SGTC_2948 9070189 3.10 9.4 µM 2.40E-4 plasma membrane duress 68 9.57E-4
16 SGTC_2551 apiole 2.98 73.9 µM 3.93E-4 31 0.00142
17 SGTC_2827 7993847 2.97 71.4 µM 4.22E-4 62 0.00150
18 SGTC_86 0417-1630 2.94 471.9 µM 4.63E-4 ubiquinone biosynthesis & proteasome 233 0.00162
19 SGTC_2954 9074818 2.84 52.7 µM 6.92E-4 86 0.00223
20 SGTC_2103 5378138 2.84 10.0 µM 7.17E-4 74 0.00229

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.167 2.95E-22 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.157 5.53E-20 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.154 3.08E-19 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.144 4.83E-17 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.133 1.26E-14 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.131 2.28E-14 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.130 3.79E-14 YMR039C SUB1 hom Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress
0.130 4.93E-14 YDL171C GLT1 hom NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
0.124 5.67E-13 YCR072C RSA4 het WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
0.122 1.24E-12 YLL046C RNP1 hom Ribonucleoprotein that contains two RNA recognition motifs (RRM)
0.119 4.41E-12 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.119 4.49E-12 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.118 7.13E-12 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.116 1.71E-11 YGR127W_p YGR127W_p hom Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
0.115 2.18E-11 YGL149W_d YGL149W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C.