YER052C / HOM3

Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YER052C / HOM3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:37.20|P-value:5.78E-299 ID:SGTC_18|Compound:0111-0177|FD-Score:5.73|P-value:6.54E-9 ID:SGTC_34|Compound:0469-0683|FD-Score:5.33|P-value:6.17E-8 ID:SGTC_50|Compound:0929-0078|FD-Score:-4.00|P-value:3.41E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.65|P-value:1.43E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.00|P-value:3.73E-5 ID:SGTC_90|Compound:0870-0863|FD-Score:4.16|P-value:1.85E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:6.82|P-value:6.78E-12 ID:SGTC_122|Compound:0236-0016|FD-Score:3.20|P-value:7.49E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:41.00|P-value:0 ID:SGTC_165|Compound:k038-0023|FD-Score:3.45|P-value:3.15E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:6.66|P-value:2.01E-11 ID:SGTC_236|Compound:1509-0011|FD-Score:19.80|P-value:3.06E-86 ID:SGTC_291|Compound:4130-1278|FD-Score:3.24|P-value:6.57E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.70|P-value:1.23E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.52|P-value:2.38E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.45|P-value:3.13E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:5.46|P-value:3.13E-8 ID:SGTC_386|Compound:1509-0011|FD-Score:13.70|P-value:3.69E-42 ID:SGTC_431|Compound:k089-0097|FD-Score:3.41|P-value:3.58E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:4.65|P-value:2.01E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:5.67|P-value:9.24E-9 ID:SGTC_799|Compound:1310-0557|FD-Score:3.99|P-value:3.75E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:-5.14|P-value:1.65E-7 ID:SGTC_957|Compound:1187-1561|FD-Score:-5.93|P-value:1.91E-9 ID:SGTC_961|Compound:1252-1571|FD-Score:3.45|P-value:3.10E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.58|P-value:1.88E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.27|P-value:6.00E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.94|P-value:4.67E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:4.42|P-value:5.86E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.74|P-value:1.05E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.85|P-value:6.84E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.20|P-value:7.41E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:-3.73|P-value:1.03E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:18.20|P-value:3.30E-73 ID:SGTC_1908|Compound:st060353|FD-Score:-5.06|P-value:2.43E-7 ID:SGTC_2050|Compound:5263056|FD-Score:3.44|P-value:3.24E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.41|P-value:3.68E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.93|P-value:4.80E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.13|P-value:3.49E-16 ID:SGTC_2289|Compound:7946404|FD-Score:5.17|P-value:1.51E-7 ID:SGTC_2297|Compound:7971671|FD-Score:3.56|P-value:2.12E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.42|P-value:5.80E-6 ID:SGTC_2464|Compound:5717415|FD-Score:3.73|P-value:1.10E-4 ID:SGTC_2469|Compound:5655019|FD-Score:4.68|P-value:1.77E-6 ID:SGTC_2471|Compound:5466956|FD-Score:3.47|P-value:2.97E-4 ID:SGTC_2475|Compound:5812728|FD-Score:5.19|P-value:1.30E-7 ID:SGTC_2479|Compound:5617353|FD-Score:3.80|P-value:8.33E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.18|P-value:8.00E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:4.45|P-value:5.13E-6 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.25|P-value:6.21E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.45|P-value:3.09E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-5.60|P-value:1.31E-8 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.93|P-value:4.86E-5 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.13|P-value:9.61E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:16.70|P-value:2.76E-62 ID:SGTC_2679|Compound:rapamycin|FD-Score:25.30|P-value:5.98E-139 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.63|P-value:1.57E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-4.00|P-value:3.44E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:4.41|P-value:6.16E-6 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.23|P-value:1.36E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.24|P-value:6.65E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.78|P-value:8.99E-5 ID:SGTC_2882|Compound:9058044|FD-Score:-3.44|P-value:3.14E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-3.28|P-value:5.48E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.39|P-value:3.83E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-5.04|P-value:2.77E-7 ID:SGTC_3089|Compound:9117425|FD-Score:-3.38|P-value:3.94E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.36|P-value:4.41E-4 ID:SGTC_3204|Compound:9114183|FD-Score:4.03|P-value:3.18E-5 ID:SGTC_3207|Compound:9114163|FD-Score:4.67|P-value:1.86E-6 ID:SGTC_3210|Compound:9128157|FD-Score:3.69|P-value:1.26E-4 ID:SGTC_3324|Compound:9138759|FD-Score:5.30|P-value:7.50E-8 ID:SGTC_7|Compound:0192-1582|FD-Score:37.20|P-value:5.78E-299 ID:SGTC_18|Compound:0111-0177|FD-Score:5.73|P-value:6.54E-9 ID:SGTC_34|Compound:0469-0683|FD-Score:5.33|P-value:6.17E-8 ID:SGTC_50|Compound:0929-0078|FD-Score:-4.00|P-value:3.41E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:3.78|P-value:9.06E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.65|P-value:1.43E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.00|P-value:3.73E-5 ID:SGTC_90|Compound:0870-0863|FD-Score:4.16|P-value:1.85E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:6.82|P-value:6.78E-12 ID:SGTC_122|Compound:0236-0016|FD-Score:3.20|P-value:7.49E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:41.00|P-value:0 ID:SGTC_165|Compound:k038-0023|FD-Score:3.45|P-value:3.15E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:6.66|P-value:2.01E-11 ID:SGTC_236|Compound:1509-0011|FD-Score:19.80|P-value:3.06E-86 ID:SGTC_291|Compound:4130-1278|FD-Score:3.24|P-value:6.57E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.70|P-value:1.23E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.52|P-value:2.38E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.45|P-value:3.13E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:5.46|P-value:3.13E-8 ID:SGTC_386|Compound:1509-0011|FD-Score:13.70|P-value:3.69E-42 ID:SGTC_431|Compound:k089-0097|FD-Score:3.41|P-value:3.58E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:4.65|P-value:2.01E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:5.67|P-value:9.24E-9 ID:SGTC_799|Compound:1310-0557|FD-Score:3.99|P-value:3.75E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:-5.14|P-value:1.65E-7 ID:SGTC_957|Compound:1187-1561|FD-Score:-5.93|P-value:1.91E-9 ID:SGTC_961|Compound:1252-1571|FD-Score:3.45|P-value:3.10E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.58|P-value:1.88E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.27|P-value:6.00E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.94|P-value:4.67E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:4.42|P-value:5.86E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.74|P-value:1.05E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.85|P-value:6.84E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.20|P-value:7.41E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:-3.73|P-value:1.03E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:18.20|P-value:3.30E-73 ID:SGTC_1908|Compound:st060353|FD-Score:-5.06|P-value:2.43E-7 ID:SGTC_2050|Compound:5263056|FD-Score:3.44|P-value:3.24E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.41|P-value:3.68E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.93|P-value:4.80E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:8.13|P-value:3.49E-16 ID:SGTC_2289|Compound:7946404|FD-Score:5.17|P-value:1.51E-7 ID:SGTC_2297|Compound:7971671|FD-Score:3.56|P-value:2.12E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.42|P-value:5.80E-6 ID:SGTC_2464|Compound:5717415|FD-Score:3.73|P-value:1.10E-4 ID:SGTC_2469|Compound:5655019|FD-Score:4.68|P-value:1.77E-6 ID:SGTC_2471|Compound:5466956|FD-Score:3.47|P-value:2.97E-4 ID:SGTC_2475|Compound:5812728|FD-Score:5.19|P-value:1.30E-7 ID:SGTC_2479|Compound:5617353|FD-Score:3.80|P-value:8.33E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.18|P-value:8.00E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:4.45|P-value:5.13E-6 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.25|P-value:6.21E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.45|P-value:3.09E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-5.60|P-value:1.31E-8 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.93|P-value:4.86E-5 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.13|P-value:9.61E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:16.70|P-value:2.76E-62 ID:SGTC_2679|Compound:rapamycin|FD-Score:25.30|P-value:5.98E-139 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.63|P-value:1.57E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-4.00|P-value:3.44E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:4.41|P-value:6.16E-6 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.23|P-value:1.36E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.24|P-value:6.65E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.78|P-value:8.99E-5 ID:SGTC_2882|Compound:9058044|FD-Score:-3.44|P-value:3.14E-4 ID:SGTC_2912|Compound:7964298|FD-Score:-3.28|P-value:5.48E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.39|P-value:3.83E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-5.04|P-value:2.77E-7 ID:SGTC_3089|Compound:9117425|FD-Score:-3.38|P-value:3.94E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.36|P-value:4.41E-4 ID:SGTC_3204|Compound:9114183|FD-Score:4.03|P-value:3.18E-5 ID:SGTC_3207|Compound:9114163|FD-Score:4.67|P-value:1.86E-6 ID:SGTC_3210|Compound:9128157|FD-Score:3.69|P-value:1.26E-4 ID:SGTC_3324|Compound:9138759|FD-Score:5.30|P-value:7.50E-8

Top fitness defect scores for YER052C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_163 1327-0036 41.00 7.5 µM 0 1 0
2 SGTC_7 0192-1582 37.20 4.2 µM 5.78E-299 1 2.41E-303
3 SGTC_2679 rapamycin 25.30 1.0 nM 5.98E-139 2 5.45E-141
4 SGTC_236 1509-0011 19.80 5.3 µM 3.06E-86 56 1.66E-87
5 SGTC_1844 rapamycin 18.20 4.0 nM 3.30E-73 7 2.78E-74
6 SGTC_2651 1327-0036 16.70 5.0 µM 2.76E-62 2 3.38E-63
7 SGTC_386 1509-0011 13.70 3.1 µM 3.69E-42 27 8.95E-43
8 SGTC_2233 sulbentine 8.13 40.4 µM 3.49E-16 13 2.06E-16
9 SGTC_107 0358-0022 6.82 2.7 µM 6.78E-12 SWF1 & branched chain AA biosynthesis 12 4.64E-12
10 SGTC_195 4234-0266 6.66 16.6 µM 2.01E-11 5 1.40E-11
11 SGTC_18 0111-0177 5.73 500.0 nM 6.54E-9 24 4.96E-9
12 SGTC_538 2922-0838 5.67 53.3 µM 9.24E-9 Golgi 8 7.05E-9
13 SGTC_371 0195-0006 5.46 7.4 µM 3.13E-8 5 2.43E-8
14 SGTC_34 0469-0683 5.33 81.8 µM 6.17E-8 SWF1 & branched chain AA biosynthesis 13 4.84E-8
15 SGTC_3324 9138759 5.30 60.8 µM 7.50E-8 5 5.90E-8
16 SGTC_2475 5812728 5.19 15.0 µM 1.30E-7 mitochondrial processes 9 1.03E-7
17 SGTC_2289 7946404 5.17 165.1 µM 1.51E-7 52 1.20E-7
18 SGTC_2469 5655019 4.68 90.8 µM 1.77E-6 14 1.46E-6
19 SGTC_3207 9114163 4.67 49.5 µM 1.86E-6 14 1.54E-6
20 SGTC_534 k292-0788 4.65 63.4 µM 2.01E-6 mitochondrial processes 15 1.67E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.690 0 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.472 4.70E-185 YDR158W HOM2 hom Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.404 9.44E-132 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.397 8.94E-127 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.382 1.02E-116 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.377 6.55E-114 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.372 1.67E-110 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.343 7.27E-93 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.341 9.22E-92 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.324 1.35E-82 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.322 9.11E-82 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.317 3.79E-79 YBR049C REB1 het RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
0.311 2.54E-76 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.302 1.38E-71 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.300 1.59E-70 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB