YER056C / FCY2

Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER056C / FCY2

Click on Significant Values for Screen Details ID:SGTC_61|Compound:0978-8327|FD-Score:-3.19|P-value:3.15E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:8.64|P-value:1.73E-21 ID:SGTC_293|Compound:4049-0273|FD-Score:3.13|P-value:2.69E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.09|P-value:3.18E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.37|P-value:1.51E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.11|P-value:2.98E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:5.14|P-value:8.12E-9 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.84|P-value:1.92E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:2.93|P-value:6.05E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.05|P-value:3.69E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:2.96|P-value:5.38E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.11|P-value:4.36E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.91|P-value:6.13E-8 ID:SGTC_1950|Compound:st077106|FD-Score:3.29|P-value:1.40E-4 ID:SGTC_1952|Compound:st077036|FD-Score:-3.29|P-value:2.16E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.12|P-value:4.19E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-2.94|P-value:8.38E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.04|P-value:4.33E-6 ID:SGTC_1997|Compound:st073024|FD-Score:3.18|P-value:2.17E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.13|P-value:2.66E-4 ID:SGTC_2116|Compound:5511452|FD-Score:-3.23|P-value:2.74E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.65|P-value:2.91E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.38|P-value:9.44E-5 ID:SGTC_2395|Compound:5646831|FD-Score:2.89|P-value:6.93E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.46|P-value:6.73E-5 ID:SGTC_2456|Compound:5770359|FD-Score:3.52|P-value:5.09E-5 ID:SGTC_2459|Compound:5811157|FD-Score:-3.03|P-value:6.02E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.16|P-value:2.36E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.90|P-value:6.71E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.07|P-value:5.09E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.19|P-value:2.01E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.40|P-value:8.84E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.29|P-value:2.14E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-2.96|P-value:7.61E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-2.91|P-value:9.31E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.37|P-value:9.83E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-4.21|P-value:2.99E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.74|P-value:2.95E-5 ID:SGTC_2837|Compound:9008476|FD-Score:3.90|P-value:8.58E-6 ID:SGTC_2913|Compound:7966556|FD-Score:3.29|P-value:1.39E-4 ID:SGTC_3237|Compound:9133362|FD-Score:2.95|P-value:5.42E-4 ID:SGTC_3259|Compound:9138485|FD-Score:2.92|P-value:6.15E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.18|P-value:3.37E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.19|P-value:3.15E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:8.64|P-value:1.73E-21 ID:SGTC_293|Compound:4049-0273|FD-Score:3.13|P-value:2.69E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:3.09|P-value:3.18E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.37|P-value:1.51E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:3.11|P-value:2.98E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:5.14|P-value:8.12E-9 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.84|P-value:1.92E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:2.93|P-value:6.05E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.05|P-value:3.69E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:2.96|P-value:5.38E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.11|P-value:4.36E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.91|P-value:6.13E-8 ID:SGTC_1950|Compound:st077106|FD-Score:3.29|P-value:1.40E-4 ID:SGTC_1952|Compound:st077036|FD-Score:-3.29|P-value:2.16E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.12|P-value:4.19E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-2.94|P-value:8.38E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.04|P-value:4.33E-6 ID:SGTC_1997|Compound:st073024|FD-Score:3.18|P-value:2.17E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.13|P-value:2.66E-4 ID:SGTC_2116|Compound:5511452|FD-Score:-3.23|P-value:2.74E-4 ID:SGTC_2377|Compound:9073351|FD-Score:3.65|P-value:2.91E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.38|P-value:9.44E-5 ID:SGTC_2395|Compound:5646831|FD-Score:2.89|P-value:6.93E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.46|P-value:6.73E-5 ID:SGTC_2456|Compound:5770359|FD-Score:3.52|P-value:5.09E-5 ID:SGTC_2459|Compound:5811157|FD-Score:-3.03|P-value:6.02E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.16|P-value:2.36E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.90|P-value:6.71E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.07|P-value:5.09E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:4.19|P-value:2.01E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.40|P-value:8.84E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.29|P-value:2.14E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-2.96|P-value:7.61E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-2.91|P-value:9.31E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.37|P-value:9.83E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-4.21|P-value:2.99E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.74|P-value:2.95E-5 ID:SGTC_2837|Compound:9008476|FD-Score:3.90|P-value:8.58E-6 ID:SGTC_2913|Compound:7966556|FD-Score:3.29|P-value:1.39E-4 ID:SGTC_3237|Compound:9133362|FD-Score:2.95|P-value:5.42E-4 ID:SGTC_3259|Compound:9138485|FD-Score:2.92|P-value:6.15E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.18|P-value:3.37E-4

Top fitness defect scores for YER056C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_245 methotrexate 8.64 289.6 µM 1.73E-21 3 2.90E-18
2 SGTC_816 0325-0417 5.14 81.3 µM 8.12E-9 3 1.35E-7
3 SGTC_2603 homopterocarpin 4.19 100.0 µM 2.01E-6 29 1.40E-5
4 SGTC_1985 st071382 4.04 65.5 µM 4.33E-6 16 2.67E-5
5 SGTC_2837 9008476 3.90 45.5 µM 8.58E-6 38 4.76E-5
6 SGTC_2377 9073351 3.65 62.6 µM 2.91E-5 ergosterol biosynthesis 28 1.33E-4
7 SGTC_2456 5770359 3.52 74.5 µM 5.09E-5 24 2.13E-4
8 SGTC_2398 5228213 3.46 48.8 µM 6.73E-5 iron homeostasis 58 2.70E-4
9 SGTC_2611 pyromycin 3.40 23.9 µM 8.84E-5 60 3.40E-4
10 SGTC_2380 9035439 3.38 200.0 µM 9.44E-5 endomembrane recycling 50 3.59E-4
11 SGTC_2779 methotrexate 3.37 400.0 µM 9.83E-5 44 3.72E-4
12 SGTC_2913 7966556 3.29 36.6 µM 1.39E-4 60S ribosome export 32 4.97E-4
13 SGTC_1950 st077106 3.29 7.2 µM 1.40E-4 RNA processing & uracil transport 54 4.99E-4
14 SGTC_609 0986-0026 3.24 86.3 µM 1.74E-4 calcium & mitochondrial duress 60 6.00E-4
15 SGTC_1997 st073024 3.18 93.8 µM 2.17E-4 26 7.24E-4
16 SGTC_2473 5854593 3.16 8.9 µM 2.36E-4 32 7.76E-4
17 SGTC_1998 st075797 3.13 76.8 µM 2.66E-4 mitochondrial response to ROS 43 8.60E-4
18 SGTC_293 4049-0273 3.13 38.5 µM 2.69E-4 41 8.69E-4
19 SGTC_815 1582-0027 3.11 36.9 µM 2.98E-4 71 9.46E-4
20 SGTC_378 3970-0959 3.09 8.3 µM 3.18E-4 45 9.99E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.307 3.37E-74 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.211 4.28E-35 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.200 1.13E-31 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.177 6.12E-25 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.170 4.59E-23 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.167 2.17E-22 YLR267W_p BOP2_p hom Protein of unknown function
0.166 2.91E-22 YDR399W HPT1 hom Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
0.155 1.63E-19 YNL256W FOL1 het Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
0.142 1.44E-16 YKL072W STB6 hom Protein that binds Sin3p in a two-hybrid assay
0.141 2.07E-16 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.139 6.45E-16 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.137 1.67E-15 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.137 1.67E-15 YGL102C_d YGL102C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
0.135 3.96E-15 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.132 1.60E-14 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs