YER068W / MOT2

Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications

Zygosity: Homozygous strain
fixedexpanded
Profile for YER068W / MOT2

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:3.33|P-value:6.88E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.25|P-value:7.61E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.75|P-value:1.26E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.62|P-value:2.02E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.76|P-value:1.21E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:3.68|P-value:2.02E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:-4.21|P-value:2.06E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-5.52|P-value:3.89E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.73|P-value:3.03E-11 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.49|P-value:3.25E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.97|P-value:6.77E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.61|P-value:2.61E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.47|P-value:3.58E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.89|P-value:9.23E-5 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.56|P-value:3.10E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.20|P-value:8.92E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.11|P-value:3.81E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.28|P-value:8.14E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.40|P-value:5.50E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.60|P-value:2.76E-4 ID:SGTC_1927|Compound:st060202|FD-Score:5.26|P-value:2.06E-7 ID:SGTC_1938|Compound:st074717|FD-Score:-3.78|P-value:1.13E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.23|P-value:9.58E-4 ID:SGTC_2282|Compound:7944268|FD-Score:4.29|P-value:1.83E-5 ID:SGTC_2294|Compound:7971431|FD-Score:3.78|P-value:1.39E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-4.21|P-value:2.04E-5 ID:SGTC_2483|Compound:5729050|FD-Score:-4.20|P-value:2.13E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-10.90|P-value:2.38E-26 ID:SGTC_2809|Compound:7964133|FD-Score:-5.24|P-value:1.68E-7 ID:SGTC_2896|Compound:9052656|FD-Score:-3.82|P-value:9.77E-5 ID:SGTC_2919|Compound:7978188|FD-Score:-3.31|P-value:6.09E-4 ID:SGTC_2920|Compound:7982904|FD-Score:-3.46|P-value:3.66E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.63|P-value:2.47E-4 ID:SGTC_3100|Compound:9120536|FD-Score:-3.21|P-value:8.67E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.32|P-value:5.97E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.48|P-value:3.36E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-3.41|P-value:4.29E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:3.33|P-value:6.88E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.25|P-value:7.61E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.75|P-value:1.26E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.62|P-value:2.02E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.76|P-value:1.21E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:3.68|P-value:2.02E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:-4.21|P-value:2.06E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-5.52|P-value:3.89E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.73|P-value:3.03E-11 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.49|P-value:3.25E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.97|P-value:6.77E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.61|P-value:2.61E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.47|P-value:3.58E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.89|P-value:9.23E-5 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.56|P-value:3.10E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.20|P-value:8.92E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.11|P-value:3.81E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.28|P-value:8.14E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.40|P-value:5.50E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.60|P-value:2.76E-4 ID:SGTC_1927|Compound:st060202|FD-Score:5.26|P-value:2.06E-7 ID:SGTC_1938|Compound:st074717|FD-Score:-3.78|P-value:1.13E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.23|P-value:9.58E-4 ID:SGTC_2282|Compound:7944268|FD-Score:4.29|P-value:1.83E-5 ID:SGTC_2294|Compound:7971431|FD-Score:3.78|P-value:1.39E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-4.21|P-value:2.04E-5 ID:SGTC_2483|Compound:5729050|FD-Score:-4.20|P-value:2.13E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-10.90|P-value:2.38E-26 ID:SGTC_2809|Compound:7964133|FD-Score:-5.24|P-value:1.68E-7 ID:SGTC_2896|Compound:9052656|FD-Score:-3.82|P-value:9.77E-5 ID:SGTC_2919|Compound:7978188|FD-Score:-3.31|P-value:6.09E-4 ID:SGTC_2920|Compound:7982904|FD-Score:-3.46|P-value:3.66E-4 ID:SGTC_2926|Compound:9010726|FD-Score:3.63|P-value:2.47E-4 ID:SGTC_3100|Compound:9120536|FD-Score:-3.21|P-value:8.67E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.32|P-value:5.97E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.48|P-value:3.36E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-3.41|P-value:4.29E-4

Top fitness defect scores for YER068W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1927 st060202 5.26 13.8 µM 2.06E-7 9 7.23E-8
2 SGTC_2282 7944268 4.29 200.0 µM 1.83E-5 RPP1 & pyrimidine depletion 20 8.84E-6
3 SGTC_1516 st013856 4.11 110.0 µM 3.81E-5 12 1.94E-5
4 SGTC_582 0987-0078 3.97 156.0 µM 6.77E-5 calcium & mitochondrial duress 71 3.58E-5
5 SGTC_1244 0416-0015 3.89 52.5 µM 9.23E-5 ERG2 33 4.98E-5
6 SGTC_2294 7971431 3.78 200.0 µM 1.39E-4 16 7.72E-5
7 SGTC_211 3455-0679 3.68 143.5 µM 2.02E-4 27 1.15E-4
8 SGTC_2926 9010726 3.63 65.2 µM 2.47E-4 41 1.43E-4
9 SGTC_691 1548-0686 3.61 185.0 µM 2.61E-4 51 1.52E-4
10 SGTC_1921 5740136 3.60 50.0 µM 2.76E-4 TRP & mitochondrial translation 73 1.61E-4
11 SGTC_1394 3474-0001 3.56 65.7 µM 3.10E-4 endomembrane recycling 51 1.82E-4
12 SGTC_1806 st050005 3.40 60.3 µM 5.50E-4 33 3.37E-4
13 SGTC_39 0986-0283 3.33 58.4 µM 6.88E-4 calcium & mitochondrial duress 60 4.28E-4
14 SGTC_1627 st003713 3.28 25.6 µM 8.14E-4 calcium & mitochondrial duress 117 5.12E-4
15 SGTC_2016 5121758 3.23 140.0 µM 9.58E-4 redox potentiating 87 6.10E-4
16 SGTC_1263 0774-1208 3.20 64.8 µM 0.00108 91 6.91E-4
17 SGTC_2593 nobiletin 3.19 86.2 µM 0.00110 57 7.06E-4
18 SGTC_141 0443-0274 3.19 32.3 µM 0.00111 mitochondrial response to ROS 89 7.14E-4
19 SGTC_2601 securinine 3.09 100.0 µM 0.00153 65 0.00101
20 SGTC_1348 1493-0373 3.08 93.8 µM 0.00159 49 0.00105

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.324 5.59E-83 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.263 4.22E-54 YLR395C COX8 hom Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.249 8.48E-49 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.231 7.82E-42 YHR194W MDM31 hom Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
0.222 1.24E-38 YGR015C_p YGR015C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.196 2.21E-30 YJL043W_p YJL043W_p hom Putative protein of unknown function; YJL043W is a non-essential gene
0.187 1.05E-27 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.184 7.22E-27 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.179 1.64E-25 YNL220W ADE12 hom Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
0.174 2.87E-24 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.171 1.51E-23 YGL144C ROG1 hom Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication
0.168 1.48E-22 YIL115C NUP159 het FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
0.166 3.38E-22 YNR067C DSE4 hom Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother
0.152 6.77E-19 YPL100W ATG21 hom Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein
0.150 2.66E-18 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7