YER069W / ARG5,6

Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine

Zygosity: Homozygous strain
fixedexpanded
Profile for YER069W / ARG5,6

Click on Significant Values for Screen Details ID:SGTC_54|Compound:0159-0055|FD-Score:4.77|P-value:1.04E-7 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.00|P-value:7.06E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.17|P-value:9.35E-12 ID:SGTC_530|Compound:4358-1714|FD-Score:4.89|P-value:5.28E-8 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.97|P-value:5.71E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.30|P-value:1.70E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.58|P-value:2.69E-7 ID:SGTC_827|Compound:3253-0474|FD-Score:3.22|P-value:2.33E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-3.21|P-value:2.17E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.31|P-value:1.20E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:2.89|P-value:8.51E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.52|P-value:5.77E-5 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.07|P-value:3.89E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-3.96|P-value:7.07E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.58|P-value:5.08E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.82|P-value:1.62E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.68|P-value:2.84E-5 ID:SGTC_1753|Compound:st043925|FD-Score:3.53|P-value:6.40E-5 ID:SGTC_1756|Compound:st041558|FD-Score:2.87|P-value:9.04E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.99|P-value:5.29E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:7.38|P-value:4.73E-16 ID:SGTC_2834|Compound:9001755|FD-Score:-2.84|P-value:9.25E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.39|P-value:1.04E-4 ID:SGTC_2998|Compound:9066762|FD-Score:4.34|P-value:1.17E-6 ID:SGTC_2999|Compound:9069765|FD-Score:-4.21|P-value:2.02E-6 ID:SGTC_3019|Compound:9081971|FD-Score:3.24|P-value:2.17E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.39|P-value:1.15E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.91|P-value:1.08E-5 ID:SGTC_3143|Compound:9086658|FD-Score:2.91|P-value:7.94E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-2.91|P-value:7.19E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-2.86|P-value:8.67E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.76|P-value:1.88E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-6.46|P-value:7.87E-13 ID:SGTC_3211|Compound:9128781|FD-Score:15.30|P-value:7.65E-63 ID:SGTC_3283|Compound:9109332|FD-Score:-3.43|P-value:8.46E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:4.77|P-value:1.04E-7 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.00|P-value:7.06E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.17|P-value:9.35E-12 ID:SGTC_530|Compound:4358-1714|FD-Score:4.89|P-value:5.28E-8 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.97|P-value:5.71E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.30|P-value:1.70E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.58|P-value:2.69E-7 ID:SGTC_827|Compound:3253-0474|FD-Score:3.22|P-value:2.33E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-3.21|P-value:2.17E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.31|P-value:1.20E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:2.89|P-value:8.51E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.52|P-value:5.77E-5 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.07|P-value:3.89E-4 ID:SGTC_1626|Compound:st003712|FD-Score:-3.96|P-value:7.07E-6 ID:SGTC_1630|Compound:st005209|FD-Score:3.58|P-value:5.08E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.82|P-value:1.62E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.68|P-value:2.84E-5 ID:SGTC_1753|Compound:st043925|FD-Score:3.53|P-value:6.40E-5 ID:SGTC_1756|Compound:st041558|FD-Score:2.87|P-value:9.04E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.99|P-value:5.29E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:7.38|P-value:4.73E-16 ID:SGTC_2834|Compound:9001755|FD-Score:-2.84|P-value:9.25E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.39|P-value:1.04E-4 ID:SGTC_2998|Compound:9066762|FD-Score:4.34|P-value:1.17E-6 ID:SGTC_2999|Compound:9069765|FD-Score:-4.21|P-value:2.02E-6 ID:SGTC_3019|Compound:9081971|FD-Score:3.24|P-value:2.17E-4 ID:SGTC_3123|Compound:9126162|FD-Score:3.39|P-value:1.15E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.91|P-value:1.08E-5 ID:SGTC_3143|Compound:9086658|FD-Score:2.91|P-value:7.94E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-2.91|P-value:7.19E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-2.86|P-value:8.67E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.76|P-value:1.88E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-6.46|P-value:7.87E-13 ID:SGTC_3211|Compound:9128781|FD-Score:15.30|P-value:7.65E-63 ID:SGTC_3283|Compound:9109332|FD-Score:-3.43|P-value:8.46E-5

Top fitness defect scores for YER069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3211 9128781 15.30 49.5 µM 7.65E-63 1 3.05E-53
2 SGTC_2758 dehydroepiandrosterone 7.38 69.3 µM 4.73E-16 17 7.96E-14
3 SGTC_453 enantio-paf c16 6.17 10.0 µM 9.35E-12 40 3.39E-10
4 SGTC_530 4358-1714 4.89 28.0 µM 5.28E-8 19 5.07E-7
5 SGTC_54 0159-0055 4.77 9.0 µM 1.04E-7 ERG2 31 9.04E-7
6 SGTC_2998 9066762 4.34 71.4 µM 1.17E-6 RPP1 & pyrimidine depletion 20 7.05E-6
7 SGTC_392 N-ethylmaleimide 4.00 8.3 µM 7.06E-6 RNA pol III & RNase P/MRP 13 3.23E-5
8 SGTC_3135 9127742 3.91 49.5 µM 1.08E-5 24 4.63E-5
9 SGTC_1725 st036284 3.82 20.6 µM 1.62E-5 28 6.55E-5
10 SGTC_1630 st005209 3.58 31.3 µM 5.08E-5 36 1.73E-4
11 SGTC_1753 st043925 3.53 64.0 µM 6.40E-5 15 2.11E-4
12 SGTC_3123 9126162 3.39 49.5 µM 1.15E-4 33 3.48E-4
13 SGTC_676 falcarinol 3.30 140.0 µM 1.70E-4 80 4.85E-4
14 SGTC_3019 9081971 3.24 49.5 µM 2.17E-4 31 5.97E-4
15 SGTC_827 3253-0474 3.22 693.0 µM 2.33E-4 48 6.35E-4
16 SGTC_3143 9086658 2.91 49.5 µM 7.94E-4 113 0.00181
17 SGTC_1130 3454-2858 2.89 15.9 µM 8.51E-4 NEO1 145 0.00192
18 SGTC_1756 st041558 2.87 35.5 µM 9.04E-4 37 0.00202
19 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 2.80 100.0 µM 0.00118 76 0.00254
20 SGTC_1127 asiatic acid 2.80 681.4 nM 0.00120 97 0.00258

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.415 7.66E-140 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.254 1.30E-50 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.226 4.76E-40 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.222 1.34E-38 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.217 6.46E-37 YKL151C YKL151C hom NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.216 7.61E-37 YBR265W TSC10 het 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.215 1.77E-36 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.193 2.06E-29 YBR041W FAT1 hom Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
0.187 1.15E-27 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.182 2.68E-26 YOL058W ARG1 hom Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.168 1.40E-22 YBR062C_p YBR062C_p hom Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway.
0.159 2.22E-20 YJL122W ALB1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
0.158 3.77E-20 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.157 4.86E-20 YDR063W AIM7 hom Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
0.156 1.03E-19 YJL065C DLS1 hom Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication