YER088C / DOT6

Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER088C / DOT6

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.31|P-value:4.78E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.96|P-value:3.71E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.74|P-value:1.04E-6 ID:SGTC_298|Compound:1391-0729|FD-Score:3.36|P-value:3.96E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.66|P-value:1.27E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:3.22|P-value:6.58E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:3.84|P-value:6.19E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:3.76|P-value:8.36E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:3.82|P-value:6.76E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:3.87|P-value:5.54E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.61|P-value:1.53E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:5.04|P-value:2.18E-7 ID:SGTC_923|Compound:1900-2434|FD-Score:4.81|P-value:7.29E-7 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.79|P-value:7.57E-5 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.33|P-value:4.47E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:8.06|P-value:2.70E-16 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.65|P-value:9.79E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-4.06|P-value:1.74E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.45|P-value:2.84E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.59|P-value:2.16E-6 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.35|P-value:6.78E-6 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.43|P-value:3.08E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:4.14|P-value:1.71E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.55|P-value:1.97E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.11|P-value:9.55E-4 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.21|P-value:6.89E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.20|P-value:6.98E-4 ID:SGTC_1663|Compound:st014140|FD-Score:3.34|P-value:4.24E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.85|P-value:5.85E-5 ID:SGTC_1683|Compound:st010134|FD-Score:3.52|P-value:2.15E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.74|P-value:9.12E-5 ID:SGTC_1860|Compound:st056228|FD-Score:4.11|P-value:1.94E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-3.26|P-value:4.36E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.40|P-value:3.40E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.87|P-value:5.39E-5 ID:SGTC_2093|Compound:5331342|FD-Score:-3.16|P-value:6.10E-4 ID:SGTC_2097|Compound:5376186|FD-Score:6.27|P-value:1.58E-10 ID:SGTC_2102|Compound:5377816|FD-Score:3.52|P-value:2.17E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.69|P-value:8.22E-5 ID:SGTC_2185|Compound:6113692|FD-Score:4.38|P-value:5.80E-6 ID:SGTC_2221|Compound:6807166|FD-Score:4.54|P-value:2.71E-6 ID:SGTC_2238|Compound:6671620|FD-Score:6.13|P-value:3.78E-10 ID:SGTC_2376|Compound:9072922|FD-Score:3.51|P-value:2.31E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.58|P-value:1.75E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.32|P-value:4.54E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.34|P-value:4.33E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.69|P-value:8.48E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.59|P-value:1.26E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-4.45|P-value:2.91E-6 ID:SGTC_2516|Compound:gossypol|FD-Score:3.63|P-value:1.44E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.47|P-value:2.67E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.86|P-value:5.67E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:5.20|P-value:9.41E-8 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.41|P-value:3.33E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.93|P-value:4.29E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:8.12E-4 ID:SGTC_2835|Compound:9006588|FD-Score:3.46|P-value:2.79E-4 ID:SGTC_2911|Compound:7942208|FD-Score:3.13|P-value:9.08E-4 ID:SGTC_2944|Compound:9067346|FD-Score:4.11|P-value:1.96E-5 ID:SGTC_2967|Compound:9089538|FD-Score:7.13|P-value:4.00E-13 ID:SGTC_3047|Compound:9092122|FD-Score:3.91|P-value:4.52E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.74|P-value:9.20E-5 ID:SGTC_3206|Compound:9114350|FD-Score:3.39|P-value:3.59E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-4.36|P-value:4.46E-6 ID:SGTC_3217|Compound:9129673|FD-Score:-3.11|P-value:7.49E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.31|P-value:7.87E-6 ID:SGTC_3221|Compound:9128209|FD-Score:6.79|P-value:4.45E-12 ID:SGTC_3223|Compound:9129970|FD-Score:-3.60|P-value:1.18E-4 ID:SGTC_3255|Compound:9139072|FD-Score:3.27|P-value:5.48E-4 ID:SGTC_3301|Compound:9125354|FD-Score:3.38|P-value:3.66E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.21|P-value:6.83E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.31|P-value:4.78E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.96|P-value:3.71E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.74|P-value:1.04E-6 ID:SGTC_298|Compound:1391-0729|FD-Score:3.36|P-value:3.96E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.66|P-value:1.27E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:3.22|P-value:6.58E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:3.84|P-value:6.19E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:3.76|P-value:8.36E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:3.82|P-value:6.76E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:3.87|P-value:5.54E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.61|P-value:1.53E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:5.04|P-value:2.18E-7 ID:SGTC_923|Compound:1900-2434|FD-Score:4.81|P-value:7.29E-7 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.79|P-value:7.57E-5 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.33|P-value:4.47E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:8.06|P-value:2.70E-16 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.65|P-value:9.79E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-4.06|P-value:1.74E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.45|P-value:2.84E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.59|P-value:2.16E-6 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.35|P-value:6.78E-6 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.43|P-value:3.08E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:4.14|P-value:1.71E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.55|P-value:1.97E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.11|P-value:9.55E-4 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.21|P-value:6.89E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.20|P-value:6.98E-4 ID:SGTC_1663|Compound:st014140|FD-Score:3.34|P-value:4.24E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.85|P-value:5.85E-5 ID:SGTC_1683|Compound:st010134|FD-Score:3.52|P-value:2.15E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.74|P-value:9.12E-5 ID:SGTC_1860|Compound:st056228|FD-Score:4.11|P-value:1.94E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-3.26|P-value:4.36E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.40|P-value:3.40E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.87|P-value:5.39E-5 ID:SGTC_2093|Compound:5331342|FD-Score:-3.16|P-value:6.10E-4 ID:SGTC_2097|Compound:5376186|FD-Score:6.27|P-value:1.58E-10 ID:SGTC_2102|Compound:5377816|FD-Score:3.52|P-value:2.17E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.69|P-value:8.22E-5 ID:SGTC_2185|Compound:6113692|FD-Score:4.38|P-value:5.80E-6 ID:SGTC_2221|Compound:6807166|FD-Score:4.54|P-value:2.71E-6 ID:SGTC_2238|Compound:6671620|FD-Score:6.13|P-value:3.78E-10 ID:SGTC_2376|Compound:9072922|FD-Score:3.51|P-value:2.31E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.58|P-value:1.75E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.32|P-value:4.54E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.34|P-value:4.33E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.69|P-value:8.48E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.59|P-value:1.26E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-4.45|P-value:2.91E-6 ID:SGTC_2516|Compound:gossypol|FD-Score:3.63|P-value:1.44E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.47|P-value:2.67E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.86|P-value:5.67E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:5.20|P-value:9.41E-8 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.41|P-value:3.33E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.93|P-value:4.29E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.16|P-value:8.12E-4 ID:SGTC_2835|Compound:9006588|FD-Score:3.46|P-value:2.79E-4 ID:SGTC_2911|Compound:7942208|FD-Score:3.13|P-value:9.08E-4 ID:SGTC_2944|Compound:9067346|FD-Score:4.11|P-value:1.96E-5 ID:SGTC_2967|Compound:9089538|FD-Score:7.13|P-value:4.00E-13 ID:SGTC_3047|Compound:9092122|FD-Score:3.91|P-value:4.52E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.74|P-value:9.20E-5 ID:SGTC_3206|Compound:9114350|FD-Score:3.39|P-value:3.59E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-4.36|P-value:4.46E-6 ID:SGTC_3217|Compound:9129673|FD-Score:-3.11|P-value:7.49E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.31|P-value:7.87E-6 ID:SGTC_3221|Compound:9128209|FD-Score:6.79|P-value:4.45E-12 ID:SGTC_3223|Compound:9129970|FD-Score:-3.60|P-value:1.18E-4 ID:SGTC_3255|Compound:9139072|FD-Score:3.27|P-value:5.48E-4 ID:SGTC_3301|Compound:9125354|FD-Score:3.38|P-value:3.66E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.21|P-value:6.83E-4

Top fitness defect scores for YER088C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1128 3448-9350 8.06 235.0 µM 2.70E-16 1 3.82E-16
2 SGTC_2967 9089538 7.13 49.9 µM 4.00E-13 4 5.08E-13
3 SGTC_3221 9128209 6.79 49.5 µM 4.45E-12 2 5.46E-12
4 SGTC_2097 5376186 6.27 200.0 µM 1.58E-10 2 1.85E-10
5 SGTC_2238 6671620 6.13 73.2 µM 3.78E-10 5 4.36E-10
6 SGTC_2597 laccaic acid a 5.20 100.0 µM 9.41E-8 14 1.01E-7
7 SGTC_903 1134-0042 5.04 114.0 µM 2.18E-7 9 2.32E-7
8 SGTC_923 1900-2434 4.81 73.3 µM 7.29E-7 RNA processing & uracil transport 17 7.65E-7
9 SGTC_236 1509-0011 4.74 5.3 µM 1.04E-6 341 1.09E-6
10 SGTC_1181 1222-0449 4.59 220.0 µM 2.16E-6 15 2.24E-6
11 SGTC_2221 6807166 4.54 181.1 µM 2.71E-6 1 2.81E-6
12 SGTC_2185 6113692 4.38 200.0 µM 5.80E-6 ergosterol biosynthesis 10 5.95E-6
13 SGTC_1271 0832-2808 4.35 169.0 µM 6.78E-6 6 6.94E-6
14 SGTC_3218 9131285 4.31 49.5 µM 7.87E-6 endomembrane recycling 29 8.04E-6
15 SGTC_1407 3909-7909 4.14 12.3 µM 1.71E-5 60S ribosome export 11 1.73E-5
16 SGTC_1860 st056228 4.11 74.6 µM 1.94E-5 17 1.96E-5
17 SGTC_2944 9067346 4.11 25.6 µM 1.96E-5 19 1.98E-5
18 SGTC_235 0330-0087 3.96 5.0 mM 3.71E-5 TSC3-RPN4 19 3.73E-5
19 SGTC_2620 irigenin 3.93 100.0 µM 4.29E-5 41 4.31E-5
20 SGTC_3047 9092122 3.91 49.5 µM 4.52E-5 20 4.54E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 4.90E-10 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.103 2.44E-9 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.099 1.04E-8 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.097 1.99E-8 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.097 2.04E-8 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.096 2.86E-8 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.088 3.61E-7 YJL096W MRPL49 hom Mitochondrial ribosomal protein of the large subunit
0.087 4.54E-7 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.087 4.96E-7 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.086 6.40E-7 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.086 6.56E-7 YDL142C CRD1 hom Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis
0.086 6.93E-7 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.085 8.03E-7 YNL085W MKT1 hom Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress
0.084 9.95E-7 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.084 1.00E-6 YML090W_d YML090W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source