YER089C / PTC2

Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YER089C / PTC2

Click on Significant Values for Screen Details ID:SGTC_119|Compound:0121-0043|FD-Score:-3.05|P-value:3.13E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:7.22|P-value:1.25E-16 ID:SGTC_164|Compound:4100-3994|FD-Score:3.98|P-value:3.05E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:3.24|P-value:1.14E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.95|P-value:4.42E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.07|P-value:2.39E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.62|P-value:7.79E-8 ID:SGTC_304|Compound:1310-0298|FD-Score:3.06|P-value:2.45E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.53|P-value:3.65E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-3.53|P-value:3.69E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.15|P-value:2.07E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-2.77|P-value:9.37E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.61|P-value:2.43E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.82|P-value:6.83E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-2.91|P-value:5.56E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-2.95|P-value:4.63E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.81|P-value:6.69E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:3.05|P-value:2.61E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:2.86|P-value:5.50E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.96|P-value:3.26E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.78|P-value:7.61E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.19|P-value:1.42E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.20|P-value:1.63E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.59|P-value:2.21E-5 ID:SGTC_1616|Compound:st002734|FD-Score:3.10|P-value:2.07E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.85|P-value:5.76E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.13|P-value:2.20E-4 ID:SGTC_1793|Compound:st048256|FD-Score:2.91|P-value:4.61E-4 ID:SGTC_1839|Compound:st053978|FD-Score:-3.08|P-value:2.68E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.43|P-value:3.07E-7 ID:SGTC_1941|Compound:st076221|FD-Score:2.76|P-value:8.13E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.73|P-value:9.26E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.58|P-value:2.36E-5 ID:SGTC_2240|Compound:6690706|FD-Score:-3.89|P-value:6.24E-6 ID:SGTC_2244|Compound:6635614|FD-Score:-3.62|P-value:2.40E-5 ID:SGTC_2282|Compound:7944268|FD-Score:2.80|P-value:7.20E-4 ID:SGTC_2379|Compound:9035213|FD-Score:2.86|P-value:5.47E-4 ID:SGTC_2397|Compound:5161689|FD-Score:2.82|P-value:6.60E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.10|P-value:2.55E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.51|P-value:3.94E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:2.98|P-value:3.39E-4 ID:SGTC_2941|Compound:9056145|FD-Score:2.80|P-value:6.96E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.05|P-value:3.13E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:7.22|P-value:1.25E-16 ID:SGTC_164|Compound:4100-3994|FD-Score:3.98|P-value:3.05E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:3.24|P-value:1.14E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.95|P-value:4.42E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.07|P-value:2.39E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.62|P-value:7.79E-8 ID:SGTC_304|Compound:1310-0298|FD-Score:3.06|P-value:2.45E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.53|P-value:3.65E-5 ID:SGTC_401|Compound:neomycin|FD-Score:-3.53|P-value:3.69E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.15|P-value:2.07E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-2.77|P-value:9.37E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.61|P-value:2.43E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.82|P-value:6.83E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-2.91|P-value:5.56E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-2.95|P-value:4.63E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.81|P-value:6.69E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:3.05|P-value:2.61E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:2.86|P-value:5.50E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.96|P-value:3.26E-6 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.78|P-value:7.61E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.19|P-value:1.42E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.20|P-value:1.63E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.59|P-value:2.21E-5 ID:SGTC_1616|Compound:st002734|FD-Score:3.10|P-value:2.07E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.85|P-value:5.76E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.13|P-value:2.20E-4 ID:SGTC_1793|Compound:st048256|FD-Score:2.91|P-value:4.61E-4 ID:SGTC_1839|Compound:st053978|FD-Score:-3.08|P-value:2.68E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.43|P-value:3.07E-7 ID:SGTC_1941|Compound:st076221|FD-Score:2.76|P-value:8.13E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.73|P-value:9.26E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.58|P-value:2.36E-5 ID:SGTC_2240|Compound:6690706|FD-Score:-3.89|P-value:6.24E-6 ID:SGTC_2244|Compound:6635614|FD-Score:-3.62|P-value:2.40E-5 ID:SGTC_2282|Compound:7944268|FD-Score:2.80|P-value:7.20E-4 ID:SGTC_2379|Compound:9035213|FD-Score:2.86|P-value:5.47E-4 ID:SGTC_2397|Compound:5161689|FD-Score:2.82|P-value:6.60E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.10|P-value:2.55E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.51|P-value:3.94E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:2.98|P-value:3.39E-4 ID:SGTC_2941|Compound:9056145|FD-Score:2.80|P-value:6.96E-4

Top fitness defect scores for YER089C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_163 1327-0036 7.22 7.5 µM 1.25E-16 26 2.52E-13
2 SGTC_268 camptothecin 4.62 39.6 µM 7.79E-8 DNA damage response 53 1.96E-6
3 SGTC_164 4100-3994 3.98 89.6 µM 3.05E-6 32 3.49E-5
4 SGTC_1254 0550-0035 3.96 264.0 µM 3.26E-6 20 3.68E-5
5 SGTC_416 estradiol valerate 3.82 100.0 µM 6.83E-6 14 6.58E-5
6 SGTC_1607 st000305 3.59 50.6 µM 2.21E-5 21 1.66E-4
7 SGTC_2167 5876490 3.58 200.0 µM 2.36E-5 45 1.75E-4
8 SGTC_165 k038-0023 3.24 64.4 µM 1.14E-4 ubiquinone biosynthesis & proteasome 121 6.03E-4
9 SGTC_1444 4112-3581 3.19 28.0 µM 1.42E-4 39 7.18E-4
10 SGTC_1616 st002734 3.10 56.1 µM 2.07E-4 69 9.70E-4
11 SGTC_255 tunicamycin 3.07 200.5 nM 2.39E-4 unfolded protein response 134 0.00108
12 SGTC_304 1310-0298 3.06 97.0 µM 2.45E-4 cell wall 117 0.00110
13 SGTC_1169 aniline yellow 3.05 25.8 µM 2.61E-4 31 0.00116
14 SGTC_2755 lomerizine 2.98 42.7 µM 3.39E-4 72 0.00143
15 SGTC_1793 st048256 2.91 72.2 µM 4.61E-4 24 0.00182
16 SGTC_2379 9035213 2.86 200.0 µM 5.47E-4 71 0.00209
17 SGTC_1181 1222-0449 2.86 220.0 µM 5.50E-4 76 0.00210
18 SGTC_1630 st005209 2.85 31.3 µM 5.76E-4 94 0.00217
19 SGTC_2397 5161689 2.82 200.0 µM 6.60E-4 105 0.00242
20 SGTC_901 1112-0062 2.81 60.1 µM 6.69E-4 49 0.00245

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.221 2.05E-38 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.219 1.34E-37 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.211 5.82E-35 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.195 6.27E-30 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.192 4.42E-29 YDR502C SAM2 hom S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
0.191 6.38E-29 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.174 2.40E-24 YPL209C IPL1 het Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
0.174 2.60E-24 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.173 6.39E-24 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.164 1.24E-21 YDR139C RUB1 hom Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
0.160 1.01E-20 YDR381W YRA1 het RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
0.157 4.63E-20 YIL022W TIM44 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.149 4.41E-18 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.149 4.78E-18 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.144 6.11E-17 YIL003W CFD1 het Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol