YER094C / PUP3

Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER094C / PUP3

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:6.59|P-value:1.41E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:23.30|P-value:6.87E-74 ID:SGTC_137|Compound:0438-0332|FD-Score:9.34|P-value:1.76E-13 ID:SGTC_158|Compound:k038-0087|FD-Score:4.75|P-value:1.08E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-5.92|P-value:2.01E-6 ID:SGTC_187|Compound:1488-2104|FD-Score:6.52|P-value:1.89E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:5.75|P-value:3.79E-6 ID:SGTC_320|Compound:4092-0846|FD-Score:5.91|P-value:2.11E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.25|P-value:4.71E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-5.04|P-value:4.35E-5 ID:SGTC_398|Compound:bronopol|FD-Score:5.37|P-value:1.47E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.04|P-value:8.28E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:7.20|P-value:1.04E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:5.69|P-value:4.78E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:4.77|P-value:1.03E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.16|P-value:2.90E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:6.55|P-value:1.72E-7 ID:SGTC_635|Compound:k038-0028|FD-Score:4.90|P-value:6.71E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:4.81|P-value:9.07E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:-4.31|P-value:3.90E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.88|P-value:7.14E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.20|P-value:5.27E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.79|P-value:9.62E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.92|P-value:6.47E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.09|P-value:7.29E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.06|P-value:8.84E-13 ID:SGTC_1712|Compound:st032288|FD-Score:4.29|P-value:4.18E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.86|P-value:7.63E-5 ID:SGTC_1814|Compound:st052110|FD-Score:4.49|P-value:2.36E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.44|P-value:1.14E-5 ID:SGTC_1950|Compound:st077106|FD-Score:4.29|P-value:4.24E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.62|P-value:1.29E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:11.20|P-value:1.79E-18 ID:SGTC_2291|Compound:7917025|FD-Score:5.88|P-value:2.37E-6 ID:SGTC_2351|Compound:9026858|FD-Score:4.05|P-value:8.08E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:5.44|P-value:1.14E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.99|P-value:9.39E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.72|P-value:1.19E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.26|P-value:4.54E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.36|P-value:1.51E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.56|P-value:7.30E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:6.59|P-value:1.41E-7 ID:SGTC_64|Compound:000l-0018|FD-Score:23.30|P-value:6.87E-74 ID:SGTC_137|Compound:0438-0332|FD-Score:9.34|P-value:1.76E-13 ID:SGTC_158|Compound:k038-0087|FD-Score:4.75|P-value:1.08E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-5.92|P-value:2.01E-6 ID:SGTC_187|Compound:1488-2104|FD-Score:6.52|P-value:1.89E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:5.75|P-value:3.79E-6 ID:SGTC_320|Compound:4092-0846|FD-Score:5.91|P-value:2.11E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.25|P-value:4.71E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-5.04|P-value:4.35E-5 ID:SGTC_398|Compound:bronopol|FD-Score:5.37|P-value:1.47E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.04|P-value:8.28E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:7.20|P-value:1.04E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:5.69|P-value:4.78E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:4.77|P-value:1.03E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.16|P-value:2.90E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:6.55|P-value:1.72E-7 ID:SGTC_635|Compound:k038-0028|FD-Score:4.90|P-value:6.71E-5 ID:SGTC_837|Compound:0091-0265|FD-Score:4.81|P-value:9.07E-5 ID:SGTC_966|Compound:1300-0360|FD-Score:-4.31|P-value:3.90E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.88|P-value:7.14E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.20|P-value:5.27E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.79|P-value:9.62E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.92|P-value:6.47E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.09|P-value:7.29E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:9.06|P-value:8.84E-13 ID:SGTC_1712|Compound:st032288|FD-Score:4.29|P-value:4.18E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.86|P-value:7.63E-5 ID:SGTC_1814|Compound:st052110|FD-Score:4.49|P-value:2.36E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.44|P-value:1.14E-5 ID:SGTC_1950|Compound:st077106|FD-Score:4.29|P-value:4.24E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.62|P-value:1.29E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:11.20|P-value:1.79E-18 ID:SGTC_2291|Compound:7917025|FD-Score:5.88|P-value:2.37E-6 ID:SGTC_2351|Compound:9026858|FD-Score:4.05|P-value:8.08E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:5.44|P-value:1.14E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.99|P-value:9.39E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.72|P-value:1.19E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.26|P-value:4.54E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.36|P-value:1.51E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-5.56|P-value:7.30E-6

Top fitness defect scores for YER094C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_64 000l-0018 23.30 272.1 µM 6.87E-74 ubiquinone biosynthesis & proteasome 1 2.13E-120 2.27
2 SGTC_2250 alverine citrate 11.20 130.0 µM 1.79E-18 7 3.16E-29 0.28
3 SGTC_137 0438-0332 9.34 85.4 µM 1.76E-13 ubiquinone biosynthesis & proteasome 10 4.81E-21 1.43
4 SGTC_1597 7-benzyloxygramine 9.06 47.5 µM 8.84E-13 calcium & mitochondrial duress 20 6.77E-20 0.71
5 SGTC_467 ro 31-8220 7.20 72.8 µM 1.04E-8 calcium & mitochondrial duress 4 3.02E-13 0.75
6 SGTC_2249 haloperidol 6.62 116.4 µM 1.29E-7 ergosterol depletion effects on membrane 13 1.84E-11 0.04
7 SGTC_36 0335-0881 6.59 14.2 µM 1.41E-7 ubiquinone biosynthesis & proteasome 11 2.14E-11 0.06
8 SGTC_582 0987-0078 6.55 156.0 µM 1.72E-7 calcium & mitochondrial duress 3 2.94E-11 0.61
9 SGTC_187 1488-2104 6.52 32.7 µM 1.89E-7 calcium & mitochondrial duress 3 3.44E-11 0.91
10 SGTC_320 4092-0846 5.91 5.3 µM 2.11E-6 ergosterol depletion effects on membrane 9 1.73E-9 0.01
11 SGTC_2291 7917025 5.88 104.3 µM 2.37E-6 iron homeostasis 2 2.09E-9 0.33
12 SGTC_312 4092-0653 5.75 6.8 µM 3.79E-6 ergosterol depletion effects on membrane 15 4.48E-9 0.13
13 SGTC_528 3492-0019 5.69 172.0 µM 4.78E-6 calcium & mitochondrial duress 10 6.52E-9 0.00
14 SGTC_2580 diallyl disulphide 5.44 81.4 µM 1.14E-5 1 2.67E-8 0.46
15 SGTC_1880 MG-132 5.44 525.0 µM 1.14E-5 12 2.68E-8 0.01
16 SGTC_398 bronopol 5.37 33.3 µM 1.47E-5 RNA processing & uracil transport 2 4.01E-8 0.69
17 SGTC_2947 9072639 5.36 7.7 µM 1.51E-5 tubulin folding & SWR complex 5 4.20E-8 0.41
18 SGTC_574 1315-0397 5.16 40.0 µM 2.90E-5 4 1.21E-7 0.56
19 SGTC_1381 2324-0066 4.92 62.1 µM 6.47E-5 2 4.41E-7 0.15
20 SGTC_635 k038-0028 4.90 24.7 µM 6.71E-5 ubiquinone biosynthesis & proteasome 7 4.67E-7 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.581 4.21E-302 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.486 1.14E-198 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.479 6.74E-192 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.478 2.68E-191 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.478 3.57E-191 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.469 2.22E-183 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.468 9.67E-182 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.426 8.23E-148 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.390 4.45E-122 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.374 3.80E-112 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.370 4.89E-109 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.318 7.94E-80 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.289 1.97E-65 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.282 2.16E-62 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.280 1.36E-61 YPL144W POC4 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions