YER128W / VFA1

Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology

Zygosity: Homozygous strain
fixedexpanded
Profile for YER128W / VFA1

Click on Significant Values for Screen Details ID:SGTC_172|Compound:1112-0046|FD-Score:3.26|P-value:5.38E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.36|P-value:3.01E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:-3.03|P-value:9.91E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.26|P-value:6.11E-8 ID:SGTC_507|Compound:nimesulide|FD-Score:3.42|P-value:2.96E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.18|P-value:7.23E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.25|P-value:5.66E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.01|P-value:2.80E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.26|P-value:9.25E-6 ID:SGTC_782|Compound:0083-0095|FD-Score:4.56|P-value:2.28E-6 ID:SGTC_860|Compound:0443-0016|FD-Score:4.18|P-value:1.31E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:3.53|P-value:2.00E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.81|P-value:6.50E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:5.98|P-value:8.70E-10 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.33|P-value:3.39E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.05|P-value:9.26E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:-3.15|P-value:6.54E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.52|P-value:2.02E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.83|P-value:5.84E-7 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.58|P-value:1.61E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.63|P-value:1.59E-6 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.82|P-value:6.34E-5 ID:SGTC_1521|Compound:st012942|FD-Score:8.70|P-value:8.25E-19 ID:SGTC_1522|Compound:st012842|FD-Score:-3.26|P-value:4.32E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-4.18|P-value:1.00E-5 ID:SGTC_1554|Compound:st069299|FD-Score:3.90|P-value:4.52E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.98|P-value:3.14E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.79|P-value:7.11E-7 ID:SGTC_1628|Compound:st004252|FD-Score:3.31|P-value:4.54E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.23|P-value:5.96E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.30|P-value:7.79E-6 ID:SGTC_1725|Compound:st036284|FD-Score:5.54|P-value:1.18E-8 ID:SGTC_1727|Compound:st034309|FD-Score:4.10|P-value:1.85E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.13|P-value:8.49E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.21|P-value:6.37E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-3.59|P-value:1.27E-4 ID:SGTC_2085|Compound:5307070|FD-Score:5.05|P-value:1.91E-7 ID:SGTC_2239|Compound:6685329|FD-Score:3.08|P-value:9.97E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.89|P-value:4.66E-5 ID:SGTC_2335|Compound:9018011|FD-Score:-3.12|P-value:7.31E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.64|P-value:1.27E-4 ID:SGTC_2450|Compound:5739737|FD-Score:4.63|P-value:1.64E-6 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.93|P-value:3.91E-5 ID:SGTC_2819|Compound:7996655|FD-Score:5.18|P-value:9.43E-8 ID:SGTC_2826|Compound:7992541|FD-Score:3.30|P-value:4.59E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.20|P-value:6.62E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-4.37|P-value:4.23E-6 ID:SGTC_2911|Compound:7942208|FD-Score:4.72|P-value:1.04E-6 ID:SGTC_2914|Compound:7968218|FD-Score:3.42|P-value:2.97E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.60|P-value:1.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.18|P-value:7.18E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.35|P-value:3.81E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.19|P-value:5.52E-4 ID:SGTC_3254|Compound:9136651|FD-Score:-3.26|P-value:4.36E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.61|P-value:1.31E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:3.26|P-value:5.38E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.36|P-value:3.01E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:-3.03|P-value:9.91E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.26|P-value:6.11E-8 ID:SGTC_507|Compound:nimesulide|FD-Score:3.42|P-value:2.96E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.18|P-value:7.23E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.25|P-value:5.66E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.01|P-value:2.80E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.26|P-value:9.25E-6 ID:SGTC_782|Compound:0083-0095|FD-Score:4.56|P-value:2.28E-6 ID:SGTC_860|Compound:0443-0016|FD-Score:4.18|P-value:1.31E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:3.53|P-value:2.00E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.81|P-value:6.50E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:5.98|P-value:8.70E-10 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.33|P-value:3.39E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.05|P-value:9.26E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:-3.15|P-value:6.54E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:3.52|P-value:2.02E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.83|P-value:5.84E-7 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.58|P-value:1.61E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.63|P-value:1.59E-6 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.82|P-value:6.34E-5 ID:SGTC_1521|Compound:st012942|FD-Score:8.70|P-value:8.25E-19 ID:SGTC_1522|Compound:st012842|FD-Score:-3.26|P-value:4.32E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-4.18|P-value:1.00E-5 ID:SGTC_1554|Compound:st069299|FD-Score:3.90|P-value:4.52E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.98|P-value:3.14E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.79|P-value:7.11E-7 ID:SGTC_1628|Compound:st004252|FD-Score:3.31|P-value:4.54E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.23|P-value:5.96E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.30|P-value:7.79E-6 ID:SGTC_1725|Compound:st036284|FD-Score:5.54|P-value:1.18E-8 ID:SGTC_1727|Compound:st034309|FD-Score:4.10|P-value:1.85E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.13|P-value:8.49E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.21|P-value:6.37E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-3.59|P-value:1.27E-4 ID:SGTC_2085|Compound:5307070|FD-Score:5.05|P-value:1.91E-7 ID:SGTC_2239|Compound:6685329|FD-Score:3.08|P-value:9.97E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.89|P-value:4.66E-5 ID:SGTC_2335|Compound:9018011|FD-Score:-3.12|P-value:7.31E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.64|P-value:1.27E-4 ID:SGTC_2450|Compound:5739737|FD-Score:4.63|P-value:1.64E-6 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.93|P-value:3.91E-5 ID:SGTC_2819|Compound:7996655|FD-Score:5.18|P-value:9.43E-8 ID:SGTC_2826|Compound:7992541|FD-Score:3.30|P-value:4.59E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.20|P-value:6.62E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-4.37|P-value:4.23E-6 ID:SGTC_2911|Compound:7942208|FD-Score:4.72|P-value:1.04E-6 ID:SGTC_2914|Compound:7968218|FD-Score:3.42|P-value:2.97E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.60|P-value:1.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.18|P-value:7.18E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.35|P-value:3.81E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.19|P-value:5.52E-4 ID:SGTC_3254|Compound:9136651|FD-Score:-3.26|P-value:4.36E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.61|P-value:1.31E-6

Top fitness defect scores for YER128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1521 st012942 8.70 49.4 µM 8.25E-19 2 1.62E-18
2 SGTC_874 0599-0792 5.98 117.0 µM 8.70E-10 7 1.14E-9
3 SGTC_1725 st036284 5.54 20.6 µM 1.18E-8 8 1.47E-8
4 SGTC_359 0081-0058 5.26 15.9 µM 6.11E-8 60S ribosome export 15 7.37E-8
5 SGTC_2819 7996655 5.18 46.4 µM 9.43E-8 7 1.13E-7
6 SGTC_2085 5307070 5.05 144.1 µM 1.91E-7 superoxide 11 2.25E-7
7 SGTC_1155 4088-0394 4.83 48.2 µM 5.84E-7 11 6.77E-7
8 SGTC_1600 coumarin 106 4.79 71.1 µM 7.11E-7 12 8.22E-7
9 SGTC_2911 7942208 4.72 15.6 µM 1.04E-6 mitochondrial response to ROS 8 1.19E-6
10 SGTC_1468 k284-1086 4.63 134.0 µM 1.59E-6 DNA intercalators 15 1.81E-6
11 SGTC_2450 5739737 4.63 12.9 µM 1.64E-6 10 1.87E-6
12 SGTC_782 0083-0095 4.56 30.5 µM 2.28E-6 16 2.59E-6
13 SGTC_1719 st032227 4.30 49.2 µM 7.79E-6 16 8.64E-6
14 SGTC_773 4358-1457 4.26 44.6 µM 9.25E-6 35 1.02E-5
15 SGTC_860 0443-0016 4.18 25.1 µM 1.31E-5 26 1.44E-5
16 SGTC_1727 st034309 4.10 9.9 µM 1.85E-5 34 2.03E-5
17 SGTC_770 0389-1121 4.01 67.2 µM 2.80E-5 RPP1 & pyrimidine depletion 28 3.04E-5
18 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.98 54.9 µM 3.14E-5 redox potentiating 53 3.41E-5
19 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.93 3.7 µM 3.91E-5 85 4.22E-5
20 SGTC_1554 st069299 3.90 38.7 µM 4.52E-5 24 4.87E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 3.80E-12 YLR392C ART10 hom Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
0.112 7.47E-11 YPL110C GDE1 hom Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
0.110 1.59E-10 YGR242W_d YGR242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
0.105 1.14E-9 YPL261C_d YPL261C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W
0.097 2.08E-8 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.095 3.54E-8 YER157W COG3 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.087 4.15E-7 YHL032C GUT1 hom Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
0.083 1.35E-6 YNL108C_p YNL108C_p hom Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
0.079 4.26E-6 YKR012C_d YKR012C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2
0.076 9.87E-6 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.075 1.54E-5 YCR014C POL4 hom DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
0.074 1.61E-5 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.074 1.66E-5 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.072 2.72E-5 YIR009W MSL1 hom U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
0.072 3.07E-5 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication