YER171W / RAD3

5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER171W / RAD3

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:2.84|P-value:4.40E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.70|P-value:1.16E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.89|P-value:4.18E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.77|P-value:1.73E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:3.05|P-value:1.80E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.56|P-value:2.39E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:2.76|P-value:5.90E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.80|P-value:5.04E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-2.95|P-value:3.91E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:7.93|P-value:1.51E-20 ID:SGTC_339|Compound:3091-4833|FD-Score:2.90|P-value:3.32E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-2.95|P-value:3.86E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.06|P-value:3.36E-9 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.03|P-value:1.28E-6 ID:SGTC_444|Compound:curcumin|FD-Score:3.40|P-value:3.55E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.99|P-value:1.56E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:3.03|P-value:1.92E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.00|P-value:2.17E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.57|P-value:2.29E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.69|P-value:3.95E-8 ID:SGTC_825|Compound:1988-2224|FD-Score:-2.81|P-value:6.93E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-4.65|P-value:5.00E-8 ID:SGTC_994|Compound:3910-0338|FD-Score:5.02|P-value:2.41E-9 ID:SGTC_998|Compound:3996-0085|FD-Score:-4.54|P-value:9.99E-8 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.43|P-value:4.37E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:-2.90|P-value:4.71E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.73|P-value:9.86E-6 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.16|P-value:1.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.19|P-value:1.34E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.01|P-value:2.07E-4 ID:SGTC_1611|Compound:st001856|FD-Score:2.65|P-value:9.23E-4 ID:SGTC_1614|Compound:st002383|FD-Score:-3.18|P-value:1.44E-4 ID:SGTC_1633|Compound:st005664|FD-Score:2.69|P-value:8.13E-4 ID:SGTC_1634|Compound:st006576|FD-Score:2.72|P-value:7.12E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.57|P-value:1.46E-5 ID:SGTC_1715|Compound:st032160|FD-Score:3.36|P-value:4.18E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.62|P-value:1.15E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.11|P-value:1.92E-4 ID:SGTC_1750|Compound:st081859|FD-Score:8.03|P-value:5.30E-21 ID:SGTC_1757|Compound:st041620|FD-Score:2.70|P-value:7.65E-4 ID:SGTC_2019|Compound:4016004|FD-Score:2.69|P-value:7.99E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.66|P-value:4.57E-8 ID:SGTC_2096|Compound:5468907|FD-Score:3.28|P-value:6.25E-5 ID:SGTC_2196|Compound:6573537|FD-Score:2.73|P-value:6.89E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.08|P-value:1.57E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.32|P-value:5.19E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.40|P-value:5.13E-5 ID:SGTC_2623|Compound:humulene|FD-Score:-2.93|P-value:4.16E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.10|P-value:1.42E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:-2.91|P-value:4.46E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.12|P-value:1.28E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.32|P-value:2.31E-7 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.44|P-value:1.08E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-2.76|P-value:8.40E-4 ID:SGTC_2838|Compound:9002687|FD-Score:3.91|P-value:2.48E-6 ID:SGTC_2855|Compound:9016525|FD-Score:-3.39|P-value:5.32E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.19|P-value:8.24E-7 ID:SGTC_2987|Compound:9018338|FD-Score:-3.55|P-value:2.54E-5 ID:SGTC_3094|Compound:9117742|FD-Score:-2.85|P-value:5.81E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-2.72|P-value:9.57E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:2.84|P-value:4.40E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.70|P-value:1.16E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.89|P-value:4.18E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.77|P-value:1.73E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:3.05|P-value:1.80E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.56|P-value:2.39E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:2.76|P-value:5.90E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.80|P-value:5.04E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-2.95|P-value:3.91E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:7.93|P-value:1.51E-20 ID:SGTC_339|Compound:3091-4833|FD-Score:2.90|P-value:3.32E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-2.95|P-value:3.86E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.06|P-value:3.36E-9 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.03|P-value:1.28E-6 ID:SGTC_444|Compound:curcumin|FD-Score:3.40|P-value:3.55E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.99|P-value:1.56E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:3.03|P-value:1.92E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.00|P-value:2.17E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.57|P-value:2.29E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.69|P-value:3.95E-8 ID:SGTC_825|Compound:1988-2224|FD-Score:-2.81|P-value:6.93E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-4.65|P-value:5.00E-8 ID:SGTC_994|Compound:3910-0338|FD-Score:5.02|P-value:2.41E-9 ID:SGTC_998|Compound:3996-0085|FD-Score:-4.54|P-value:9.99E-8 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.43|P-value:4.37E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:-2.90|P-value:4.71E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.73|P-value:9.86E-6 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.16|P-value:1.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.19|P-value:1.34E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.01|P-value:2.07E-4 ID:SGTC_1611|Compound:st001856|FD-Score:2.65|P-value:9.23E-4 ID:SGTC_1614|Compound:st002383|FD-Score:-3.18|P-value:1.44E-4 ID:SGTC_1633|Compound:st005664|FD-Score:2.69|P-value:8.13E-4 ID:SGTC_1634|Compound:st006576|FD-Score:2.72|P-value:7.12E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.57|P-value:1.46E-5 ID:SGTC_1715|Compound:st032160|FD-Score:3.36|P-value:4.18E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.62|P-value:1.15E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.11|P-value:1.92E-4 ID:SGTC_1750|Compound:st081859|FD-Score:8.03|P-value:5.30E-21 ID:SGTC_1757|Compound:st041620|FD-Score:2.70|P-value:7.65E-4 ID:SGTC_2019|Compound:4016004|FD-Score:2.69|P-value:7.99E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.66|P-value:4.57E-8 ID:SGTC_2096|Compound:5468907|FD-Score:3.28|P-value:6.25E-5 ID:SGTC_2196|Compound:6573537|FD-Score:2.73|P-value:6.89E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.08|P-value:1.57E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.32|P-value:5.19E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.40|P-value:5.13E-5 ID:SGTC_2623|Compound:humulene|FD-Score:-2.93|P-value:4.16E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.10|P-value:1.42E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:-2.91|P-value:4.46E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.12|P-value:1.28E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.32|P-value:2.31E-7 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.44|P-value:1.08E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:-2.76|P-value:8.40E-4 ID:SGTC_2838|Compound:9002687|FD-Score:3.91|P-value:2.48E-6 ID:SGTC_2855|Compound:9016525|FD-Score:-3.39|P-value:5.32E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.19|P-value:8.24E-7 ID:SGTC_2987|Compound:9018338|FD-Score:-3.55|P-value:2.54E-5 ID:SGTC_3094|Compound:9117742|FD-Score:-2.85|P-value:5.81E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-2.72|P-value:9.57E-4

Top fitness defect scores for YER171W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1750 st081859 8.03 2.4 µM 5.30E-21 endomembrane recycling 10 4.90E-16 0.29
2 SGTC_314 1671-0008 7.93 2.7 µM 1.51E-20 1 1.07E-15 1.39
3 SGTC_994 3910-0338 5.02 138.0 µM 2.41E-9 calcium & mitochondrial duress 6 2.55E-7 0.15
4 SGTC_2759 disulfiram 4.44 3.4 µM 1.08E-7 13 4.41E-6 0.17
5 SGTC_2757 clomiphene 4.32 4.2 µM 2.31E-7 fatty acid desaturase (OLE1) 8 7.80E-6 0.24
6 SGTC_423 5-fluorouracil 4.03 457.0 µM 1.28E-6 exosome 27 2.81E-5 0.00
7 SGTC_453 enantio-paf c16 3.99 10.0 µM 1.56E-6 21 3.27E-5 0.02
8 SGTC_2838 9002687 3.91 71.4 µM 2.48E-6 fatty acid desaturase (OLE1) 14 4.64E-5 0.03
9 SGTC_1717 st033143 3.62 58.0 µM 1.15E-5 8 1.47E-4 0.02
10 SGTC_1698 5-fluorocytosine 3.57 1.1 µM 1.46E-5 exosome 18 1.76E-4 0.14
11 SGTC_444 curcumin 3.40 90.4 µM 3.55E-5 iron homeostasis 23 3.42E-4 0.02
12 SGTC_1715 st032160 3.36 10.2 µM 4.18E-5 12 3.87E-4 0.06
13 SGTC_2597 laccaic acid a 3.32 100.0 µM 5.19E-5 12 4.56E-4 0.02
14 SGTC_2096 5468907 3.28 200.0 µM 6.25E-5 5 5.24E-4 0.03
15 SGTC_2671 danthron 3.12 100.0 µM 1.28E-4 DNA damage response 27 8.99E-4 0.09
16 SGTC_2651 1327-0036 3.10 5.0 µM 1.42E-4 59 9.72E-4 0.09
17 SGTC_2308 7584454 3.08 197.6 µM 1.57E-4 24 0.00105 0.02
18 SGTC_163 1327-0036 3.05 7.5 µM 1.80E-4 69 0.00116 0.10
19 SGTC_487 nimodipine 3.03 119.0 µM 1.92E-4 3 0.00122 0.14
20 SGTC_1577 3(2'-chlorophenyl)-7-ethoxycoumarin 3.01 66.5 µM 2.07E-4 8 0.00129 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.252 7.50E-50 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.218 2.50E-37 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.211 4.39E-35 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.209 1.52E-34 YPR153W YPR153W hom Putative protein of unknown function
0.208 3.21E-34 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.199 2.60E-31 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.184 9.00E-27 YDL011C_d YDL011C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W
0.183 9.79E-27 YJL002C OST1 het Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.181 4.20E-26 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.176 8.84E-25 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.174 3.23E-24 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.168 1.00E-22 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.163 1.87E-21 YHR077C NMD2 hom Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
0.161 5.11E-21 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.161 6.59E-21 YNR017W TIM23 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel