YER172C / BRR2

RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER172C / BRR2

Click on Significant Values for Screen Details ID:SGTC_174|Compound:3553-0389|FD-Score:-2.71|P-value:4.87E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:5.69|P-value:5.01E-12 ID:SGTC_182|Compound:3448-4371|FD-Score:2.71|P-value:6.32E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.09|P-value:5.32E-7 ID:SGTC_508|Compound:roscovitine|FD-Score:-2.75|P-value:4.08E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.80|P-value:4.27E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-2.83|P-value:2.99E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:-2.81|P-value:3.18E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.94|P-value:9.01E-7 ID:SGTC_763|Compound:3453-1109|FD-Score:3.11|P-value:1.04E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:5.37|P-value:6.47E-11 ID:SGTC_912|Compound:3474-0017|FD-Score:-2.95|P-value:1.69E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.05|P-value:1.37E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.80|P-value:3.22E-9 ID:SGTC_1194|Compound:1488-0194|FD-Score:2.63|P-value:8.83E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.74|P-value:5.45E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.58|P-value:8.65E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:2.88|P-value:3.09E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.13|P-value:9.68E-5 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.31|P-value:4.06E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.91|P-value:2.62E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:2.63|P-value:8.86E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.09|P-value:1.15E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-2.66|P-value:6.04E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.07|P-value:1.29E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-2.69|P-value:5.36E-4 ID:SGTC_2365|Compound:9042475|FD-Score:2.60|P-value:9.74E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-2.56|P-value:9.28E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.17|P-value:6.03E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.85|P-value:3.43E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-2.71|P-value:5.03E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.90|P-value:2.83E-4 ID:SGTC_2716|Compound:st080858|FD-Score:2.79|P-value:4.53E-4 ID:SGTC_2866|Compound:9034245|FD-Score:2.71|P-value:6.22E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-3.03|P-value:1.18E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.71|P-value:6.15E-9 ID:SGTC_3203|Compound:9113543|FD-Score:-4.04|P-value:5.02E-7 ID:SGTC_174|Compound:3553-0389|FD-Score:-2.71|P-value:4.87E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:5.69|P-value:5.01E-12 ID:SGTC_182|Compound:3448-4371|FD-Score:2.71|P-value:6.32E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.09|P-value:5.32E-7 ID:SGTC_508|Compound:roscovitine|FD-Score:-2.75|P-value:4.08E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.80|P-value:4.27E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-2.83|P-value:2.99E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:-2.81|P-value:3.18E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.94|P-value:9.01E-7 ID:SGTC_763|Compound:3453-1109|FD-Score:3.11|P-value:1.04E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:5.37|P-value:6.47E-11 ID:SGTC_912|Compound:3474-0017|FD-Score:-2.95|P-value:1.69E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.05|P-value:1.37E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.80|P-value:3.22E-9 ID:SGTC_1194|Compound:1488-0194|FD-Score:2.63|P-value:8.83E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.74|P-value:5.45E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.58|P-value:8.65E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:2.88|P-value:3.09E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.13|P-value:9.68E-5 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.31|P-value:4.06E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.91|P-value:2.62E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:2.63|P-value:8.86E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.09|P-value:1.15E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-2.66|P-value:6.04E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.07|P-value:1.29E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-2.69|P-value:5.36E-4 ID:SGTC_2365|Compound:9042475|FD-Score:2.60|P-value:9.74E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-2.56|P-value:9.28E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.17|P-value:6.03E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.85|P-value:3.43E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-2.71|P-value:5.03E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.90|P-value:2.83E-4 ID:SGTC_2716|Compound:st080858|FD-Score:2.79|P-value:4.53E-4 ID:SGTC_2866|Compound:9034245|FD-Score:2.71|P-value:6.22E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-3.03|P-value:1.18E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.71|P-value:6.15E-9 ID:SGTC_3203|Compound:9113543|FD-Score:-4.04|P-value:5.02E-7

Top fitness defect scores for YER172C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_179 1273-0063 5.69 6.8 µM 5.01E-12 1 6.42E-9 0.71
2 SGTC_769 0302-0231 5.37 76.0 µM 6.47E-11 4 3.86E-8 0.23
3 SGTC_359 0081-0058 4.09 15.9 µM 5.32E-7 60S ribosome export 13 2.19E-5 0.05
4 SGTC_1345 1488-0192 3.31 31.4 µM 4.06E-5 4 4.74E-4 0.15
5 SGTC_1343 1487-0095 3.13 224.0 µM 9.68E-5 7 8.80E-4 0.02
6 SGTC_763 3453-1109 3.11 128.0 µM 1.04E-4 13 9.24E-4 0.02
7 SGTC_1691 st019933 3.09 84.6 µM 1.15E-4 5 9.96E-4 0.04
8 SGTC_1828 st055811 3.07 49.0 µM 1.29E-4 3 0.00108 0.01
9 SGTC_1047 2043-3044 3.05 82.0 µM 1.37E-4 19 0.00113 0.04
10 SGTC_1370 2001-0092 2.91 44.6 µM 2.62E-4 14 0.00179 0.03
11 SGTC_2708 st078559 2.90 56.0 µM 2.83E-4 9 0.00189 0.14
12 SGTC_1309 pyrazolanthrone 2.88 13.4 µM 3.09E-4 26 0.00202 0.03
13 SGTC_2574 2',4'-dihydroxychalcone 2.85 14.6 µM 3.43E-4 28 0.00217 0.04
14 SGTC_563 tetrahydrosecaminediol 2.80 614.0 µM 4.27E-4 amide catabolism 11 0.00254 0.12
15 SGTC_2716 st080858 2.79 38.6 µM 4.53E-4 60S ribosome export 14 0.00265 0.04
16 SGTC_1261 0712-0010 2.74 12.7 µM 5.45E-4 RNA processing & uracil transport 25 0.00303 6.18E-5
17 SGTC_2866 9034245 2.71 58.4 µM 6.22E-4 6 0.00333 0.01
18 SGTC_182 3448-4371 2.71 550.5 µM 6.32E-4 13 0.00337 0.02
19 SGTC_1194 1488-0194 2.63 225.0 µM 8.83E-4 15 0.00428 0.19
20 SGTC_1553 gossypol 2.63 38.6 µM 8.86E-4 9 0.00429 9.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.233 1.64E-42 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.227 1.68E-40 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.222 1.14E-38 YDR242W AMD2 hom Putative amidase
0.214 3.46E-36 YMR115W MGR3 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.214 4.56E-36 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.201 4.71E-32 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.180 9.26E-26 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.179 1.15E-25 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.176 7.42E-25 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.167 2.49E-22 YOR166C SWT1 hom RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
0.163 1.98E-21 YNR039C ZRG17 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
0.161 6.08E-21 YKL141W SDH3 het Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
0.160 1.30E-20 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.159 1.99E-20 YDL138W RGT2 hom Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
0.150 2.71E-18 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs