YER175C / TMT1

Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle

Zygosity: Homozygous strain
fixedexpanded
Profile for YER175C / TMT1

Click on Significant Values for Screen Details ID:SGTC_182|Compound:3448-4371|FD-Score:2.81|P-value:4.89E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.22|P-value:8.39E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.70|P-value:7.55E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:4.71|P-value:2.41E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:3.48|P-value:2.54E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:2.90|P-value:3.42E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.35|P-value:4.66E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:4.30|P-value:2.97E-7 ID:SGTC_592|Compound:k241-0073|FD-Score:3.28|P-value:6.40E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:2.99|P-value:2.37E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.01|P-value:2.15E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.64|P-value:2.45E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.24|P-value:7.88E-5 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.55|P-value:1.80E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:2.87|P-value:3.83E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.27|P-value:6.78E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.27|P-value:1.35E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.92|P-value:3.12E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.83|P-value:8.42E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:2.95|P-value:2.74E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:2.97|P-value:2.60E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.03|P-value:1.96E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:2.74|P-value:6.67E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.54|P-value:1.82E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.37|P-value:8.82E-5 ID:SGTC_1753|Compound:st043925|FD-Score:-2.95|P-value:5.32E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.46|P-value:2.74E-5 ID:SGTC_1834|Compound:st055981|FD-Score:2.67|P-value:8.76E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.13|P-value:1.93E-6 ID:SGTC_2125|Compound:5246583|FD-Score:2.96|P-value:2.68E-4 ID:SGTC_2148|Compound:5546263|FD-Score:2.76|P-value:6.02E-4 ID:SGTC_2154|Compound:5570129|FD-Score:4.36|P-value:2.05E-7 ID:SGTC_2171|Compound:5739187|FD-Score:3.01|P-value:2.12E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.32|P-value:5.45E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.41|P-value:3.50E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.26|P-value:7.00E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.82|P-value:4.35E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.47|P-value:2.64E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.22|P-value:4.63E-7 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.30|P-value:3.02E-7 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.90|P-value:3.45E-4 ID:SGTC_2802|Compound:7925815|FD-Score:4.24|P-value:4.24E-7 ID:SGTC_2902|Compound:5787006|FD-Score:2.65|P-value:9.16E-4 ID:SGTC_182|Compound:3448-4371|FD-Score:2.81|P-value:4.89E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.22|P-value:8.39E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.70|P-value:7.55E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:4.71|P-value:2.41E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:3.48|P-value:2.54E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:2.90|P-value:3.42E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.35|P-value:4.66E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:4.30|P-value:2.97E-7 ID:SGTC_592|Compound:k241-0073|FD-Score:3.28|P-value:6.40E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:2.99|P-value:2.37E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.01|P-value:2.15E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.64|P-value:2.45E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.24|P-value:7.88E-5 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.55|P-value:1.80E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:2.87|P-value:3.83E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.27|P-value:6.78E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.27|P-value:1.35E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.92|P-value:3.12E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.83|P-value:8.42E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:2.95|P-value:2.74E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:2.97|P-value:2.60E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.03|P-value:1.96E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:2.74|P-value:6.67E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.54|P-value:1.82E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.37|P-value:8.82E-5 ID:SGTC_1753|Compound:st043925|FD-Score:-2.95|P-value:5.32E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.46|P-value:2.74E-5 ID:SGTC_1834|Compound:st055981|FD-Score:2.67|P-value:8.76E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.13|P-value:1.93E-6 ID:SGTC_2125|Compound:5246583|FD-Score:2.96|P-value:2.68E-4 ID:SGTC_2148|Compound:5546263|FD-Score:2.76|P-value:6.02E-4 ID:SGTC_2154|Compound:5570129|FD-Score:4.36|P-value:2.05E-7 ID:SGTC_2171|Compound:5739187|FD-Score:3.01|P-value:2.12E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.32|P-value:5.45E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.41|P-value:3.50E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.26|P-value:7.00E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.82|P-value:4.35E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.47|P-value:2.64E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.22|P-value:4.63E-7 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.30|P-value:3.02E-7 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.90|P-value:3.45E-4 ID:SGTC_2802|Compound:7925815|FD-Score:4.24|P-value:4.24E-7 ID:SGTC_2902|Compound:5787006|FD-Score:2.65|P-value:9.16E-4

Top fitness defect scores for YER175C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_361 000s-0510 4.71 1.4 µM 2.41E-8 19 1.25E-6
2 SGTC_2154 5570129 4.36 200.0 µM 2.05E-7 5 6.41E-6
3 SGTC_517 1365-0151 4.30 113.0 µM 2.97E-7 29 8.53E-6
4 SGTC_2689 tacrolimus 4.30 100.0 µM 3.02E-7 36 8.63E-6
5 SGTC_2802 7925815 4.24 54.5 µM 4.24E-7 33 1.12E-5
6 SGTC_2600 tangeritin 4.22 88.0 µM 4.63E-7 28 1.20E-5
7 SGTC_2566 crystal violet carbinol 3.82 226.0 nM 4.35E-6 42 6.63E-5
8 SGTC_1075 aripiprazole 3.55 15.2 µM 1.80E-5 44 1.96E-4
9 SGTC_1455 4262-0120 3.54 131.0 µM 1.82E-5 plasma membrane duress 35 1.98E-4
10 SGTC_366 0181-0206 3.48 4.3 µM 2.54E-5 Golgi 59 2.55E-4
11 SGTC_2567 aureobasidin a 3.47 60.0 nM 2.64E-5 117 2.63E-4
12 SGTC_1783 st039459 3.46 87.5 µM 2.74E-5 Golgi 35 2.70E-4
13 SGTC_2433 5607874 3.41 27.8 µM 3.50E-5 37 3.26E-4
14 SGTC_515 4092-1064 3.35 102.0 µM 4.66E-5 plasma membrane duress 78 4.06E-4
15 SGTC_2329 6138490 3.32 200.0 µM 5.45E-5 62 4.57E-4
16 SGTC_592 k241-0073 3.28 46.5 µM 6.40E-5 38 5.17E-4
17 SGTC_1189 1431-2119 3.27 7.3 µM 6.78E-5 60 5.41E-4
18 SGTC_2437 5741531 3.26 144.2 µM 7.00E-5 68 5.54E-4
19 SGTC_1031 k035-0009 3.24 16.0 µM 7.88E-5 62 6.07E-4
20 SGTC_265 mycophenolic acid 3.22 32.9 µM 8.39E-5 RPP1 & pyrimidine depletion 49 6.36E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 7.99E-24 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.126 2.20E-13 YER085C_p YER085C_p hom Putative protein of unknown function
0.119 4.08E-12 YLR406C RPL31B hom Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
0.117 1.49E-11 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.113 4.80E-11 YNL266W_d YNL266W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C
0.111 1.23E-10 YLR285W NNT1 hom S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
0.109 2.72E-10 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.108 3.35E-10 YER106W MAM1 hom Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
0.100 7.64E-9 YJR023C_d YJR023C_d het Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
0.096 2.55E-8 YGL121C GPG1 hom Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing
0.095 3.73E-8 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.094 4.83E-8 YFR032C-B_p YFR032C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.090 1.83E-7 YDR536W STL1 hom Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
0.090 2.01E-7 YDR464W SPP41 het Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
0.087 4.57E-7 YMR141C_d YMR141C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data