YFL002C / SPB4

Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL002C / SPB4

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:5.32|P-value:1.82E-6 ID:SGTC_37|Compound:0566-0070|FD-Score:4.10|P-value:1.75E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.75|P-value:5.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.38|P-value:6.78E-5 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.89|P-value:3.92E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.59|P-value:8.73E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.69|P-value:6.54E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:8.47|P-value:8.95E-14 ID:SGTC_181|Compound:1447-1644|FD-Score:6.99|P-value:6.10E-10 ID:SGTC_216|Compound:k038-0035|FD-Score:3.56|P-value:9.58E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.78|P-value:1.54E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:5.19|P-value:3.10E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:8.51|P-value:6.99E-14 ID:SGTC_354|Compound:2783-3500|FD-Score:5.83|P-value:1.94E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.88|P-value:3.56E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:4.92|P-value:9.14E-6 ID:SGTC_580|Compound:1123-0028|FD-Score:5.38|P-value:1.40E-6 ID:SGTC_717|Compound:1532-0246|FD-Score:3.55|P-value:9.75E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.98|P-value:2.63E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.84|P-value:4.10E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:-3.93|P-value:3.48E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.93|P-value:2.63E-12 ID:SGTC_839|Compound:0122-0028|FD-Score:5.88|P-value:1.53E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:4.48|P-value:4.69E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.32|P-value:8.24E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:5.62|P-value:5.02E-7 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.70|P-value:6.30E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:6.68|P-value:3.04E-9 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.81|P-value:4.48E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.98|P-value:2.59E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.37|P-value:6.94E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.80|P-value:4.57E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.97|P-value:2.72E-4 ID:SGTC_1516|Compound:st013856|FD-Score:5.47|P-value:9.58E-7 ID:SGTC_1608|Compound:st001473|FD-Score:-3.94|P-value:3.32E-4 ID:SGTC_1615|Compound:st002656|FD-Score:8.81|P-value:9.64E-15 ID:SGTC_1644|Compound:st010134|FD-Score:4.20|P-value:1.28E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.63|P-value:7.77E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.88|P-value:3.65E-4 ID:SGTC_1812|Compound:st052057|FD-Score:4.77|P-value:1.63E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.54|P-value:3.87E-5 ID:SGTC_1871|Compound:st057448|FD-Score:4.72|P-value:2.01E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.84|P-value:4.13E-4 ID:SGTC_1930|Compound:st074634|FD-Score:7.91|P-value:3.05E-12 ID:SGTC_2041|Compound:5107442|FD-Score:5.86|P-value:1.67E-7 ID:SGTC_2109|Compound:5483026|FD-Score:6.17|P-value:3.94E-8 ID:SGTC_2111|Compound:5486399|FD-Score:4.49|P-value:4.52E-5 ID:SGTC_2132|Compound:5317594|FD-Score:4.96|P-value:7.71E-6 ID:SGTC_2186|Compound:6124449|FD-Score:5.37|P-value:1.44E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.76|P-value:5.28E-4 ID:SGTC_2277|Compound:7947813|FD-Score:4.76|P-value:1.73E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.79|P-value:1.52E-5 ID:SGTC_2348|Compound:9022543|FD-Score:7.37|P-value:7.15E-11 ID:SGTC_2356|Compound:9061756|FD-Score:3.62|P-value:8.03E-4 ID:SGTC_2378|Compound:9074213|FD-Score:4.44|P-value:5.48E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.58|P-value:8.89E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.60|P-value:8.50E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.65|P-value:8.10E-4 ID:SGTC_2534|Compound:helenine|FD-Score:7.09|P-value:3.40E-10 ID:SGTC_2544|Compound:sphondin|FD-Score:5.45|P-value:1.05E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.72|P-value:5.94E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.67|P-value:6.81E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.63|P-value:7.76E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.84|P-value:4.15E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.79|P-value:1.51E-5 ID:SGTC_2912|Compound:7964298|FD-Score:5.68|P-value:3.75E-7 ID:SGTC_2934|Compound:9036370|FD-Score:4.06|P-value:1.99E-4 ID:SGTC_2936|Compound:9038235|FD-Score:4.04|P-value:2.13E-4 ID:SGTC_2977|Compound:9011197|FD-Score:5.47|P-value:9.55E-7 ID:SGTC_2986|Compound:9022445|FD-Score:-4.21|P-value:1.38E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.70|P-value:6.24E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-4.57|P-value:3.84E-5 ID:SGTC_3087|Compound:9117406|FD-Score:3.56|P-value:9.52E-4 ID:SGTC_3106|Compound:9121286|FD-Score:4.25|P-value:1.05E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.31|P-value:9.78E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-4.64|P-value:3.08E-5 ID:SGTC_3224|Compound:9130643|FD-Score:4.05|P-value:2.11E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.09|P-value:3.45E-10 ID:SGTC_3322|Compound:9139058|FD-Score:4.71|P-value:2.07E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:5.32|P-value:1.82E-6 ID:SGTC_37|Compound:0566-0070|FD-Score:4.10|P-value:1.75E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.75|P-value:5.33E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.38|P-value:6.78E-5 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.89|P-value:3.92E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.59|P-value:8.73E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.69|P-value:6.54E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:8.47|P-value:8.95E-14 ID:SGTC_181|Compound:1447-1644|FD-Score:6.99|P-value:6.10E-10 ID:SGTC_216|Compound:k038-0035|FD-Score:3.56|P-value:9.58E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.78|P-value:1.54E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:5.19|P-value:3.10E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:8.51|P-value:6.99E-14 ID:SGTC_354|Compound:2783-3500|FD-Score:5.83|P-value:1.94E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.88|P-value:3.56E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:4.92|P-value:9.14E-6 ID:SGTC_580|Compound:1123-0028|FD-Score:5.38|P-value:1.40E-6 ID:SGTC_717|Compound:1532-0246|FD-Score:3.55|P-value:9.75E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.98|P-value:2.63E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.84|P-value:4.10E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:-3.93|P-value:3.48E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.93|P-value:2.63E-12 ID:SGTC_839|Compound:0122-0028|FD-Score:5.88|P-value:1.53E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:4.48|P-value:4.69E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.32|P-value:8.24E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:5.62|P-value:5.02E-7 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.70|P-value:6.30E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:6.68|P-value:3.04E-9 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.81|P-value:4.48E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.98|P-value:2.59E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.37|P-value:6.94E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.80|P-value:4.57E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.97|P-value:2.72E-4 ID:SGTC_1516|Compound:st013856|FD-Score:5.47|P-value:9.58E-7 ID:SGTC_1608|Compound:st001473|FD-Score:-3.94|P-value:3.32E-4 ID:SGTC_1615|Compound:st002656|FD-Score:8.81|P-value:9.64E-15 ID:SGTC_1644|Compound:st010134|FD-Score:4.20|P-value:1.28E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.63|P-value:7.77E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.88|P-value:3.65E-4 ID:SGTC_1812|Compound:st052057|FD-Score:4.77|P-value:1.63E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.54|P-value:3.87E-5 ID:SGTC_1871|Compound:st057448|FD-Score:4.72|P-value:2.01E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.84|P-value:4.13E-4 ID:SGTC_1930|Compound:st074634|FD-Score:7.91|P-value:3.05E-12 ID:SGTC_2041|Compound:5107442|FD-Score:5.86|P-value:1.67E-7 ID:SGTC_2109|Compound:5483026|FD-Score:6.17|P-value:3.94E-8 ID:SGTC_2111|Compound:5486399|FD-Score:4.49|P-value:4.52E-5 ID:SGTC_2132|Compound:5317594|FD-Score:4.96|P-value:7.71E-6 ID:SGTC_2186|Compound:6124449|FD-Score:5.37|P-value:1.44E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.76|P-value:5.28E-4 ID:SGTC_2277|Compound:7947813|FD-Score:4.76|P-value:1.73E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.79|P-value:1.52E-5 ID:SGTC_2348|Compound:9022543|FD-Score:7.37|P-value:7.15E-11 ID:SGTC_2356|Compound:9061756|FD-Score:3.62|P-value:8.03E-4 ID:SGTC_2378|Compound:9074213|FD-Score:4.44|P-value:5.48E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.58|P-value:8.89E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.60|P-value:8.50E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.65|P-value:8.10E-4 ID:SGTC_2534|Compound:helenine|FD-Score:7.09|P-value:3.40E-10 ID:SGTC_2544|Compound:sphondin|FD-Score:5.45|P-value:1.05E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.72|P-value:5.94E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.67|P-value:6.81E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.63|P-value:7.76E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.84|P-value:4.15E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.79|P-value:1.51E-5 ID:SGTC_2912|Compound:7964298|FD-Score:5.68|P-value:3.75E-7 ID:SGTC_2934|Compound:9036370|FD-Score:4.06|P-value:1.99E-4 ID:SGTC_2936|Compound:9038235|FD-Score:4.04|P-value:2.13E-4 ID:SGTC_2977|Compound:9011197|FD-Score:5.47|P-value:9.55E-7 ID:SGTC_2986|Compound:9022445|FD-Score:-4.21|P-value:1.38E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.70|P-value:6.24E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-4.57|P-value:3.84E-5 ID:SGTC_3087|Compound:9117406|FD-Score:3.56|P-value:9.52E-4 ID:SGTC_3106|Compound:9121286|FD-Score:4.25|P-value:1.05E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-4.31|P-value:9.78E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-4.64|P-value:3.08E-5 ID:SGTC_3224|Compound:9130643|FD-Score:4.05|P-value:2.11E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.09|P-value:3.45E-10 ID:SGTC_3322|Compound:9139058|FD-Score:4.71|P-value:2.07E-5

Top fitness defect scores for YFL002C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1615 st002656 8.81 86.5 µM 9.64E-15 redox potentiating 1 6.52E-19 3.36
2 SGTC_351 caspofungin 8.51 25.0 nM 6.99E-14 cell wall 6 8.92E-18 0.81
3 SGTC_163 1327-0036 8.47 7.5 µM 8.95E-14 19 1.24E-17 0.27
4 SGTC_829 1635-0120 7.93 117.0 µM 2.63E-12 DNA damage response 16 1.07E-15 0.02
5 SGTC_1930 st074634 7.91 34.3 µM 3.05E-12 RNA pol III & RNase P/MRP 5 1.30E-15 0.33
6 SGTC_2348 9022543 7.37 33.4 µM 7.15E-11 mitochondrial processes 11 8.33E-14 0.28
7 SGTC_2534 helenine 7.09 20.1 µM 3.40E-10 RSC complex & mRNA processing 10 6.50E-13 0.72
8 SGTC_3247 9135149 7.09 49.5 µM 3.45E-10 DNA damage response 6 6.62E-13 0.35
9 SGTC_181 1447-1644 6.99 8.1 µM 6.10E-10 endomembrane recycling 3 1.40E-12 0.29
10 SGTC_1050 3232-1156 6.68 69.3 µM 3.04E-9 RSC complex & mRNA processing 6 1.16E-11 0.24
11 SGTC_2109 5483026 6.17 200.0 µM 3.94E-8 RSC complex & mRNA processing 17 3.39E-10 0.06
12 SGTC_839 0122-0028 5.88 230.0 µM 1.53E-7 RSC complex & mRNA processing 13 2.01E-9 4.00E-4
13 SGTC_2041 5107442 5.86 45.3 µM 1.67E-7 RSC complex & mRNA processing 18 2.26E-9 0.41
14 SGTC_354 2783-3500 5.83 4.9 µM 1.94E-7 cell wall 15 2.76E-9 0.29
15 SGTC_2912 7964298 5.68 10.9 µM 3.75E-7 cell wall 22 6.55E-9 0.03
16 SGTC_994 3910-0338 5.62 138.0 µM 5.02E-7 calcium & mitochondrial duress 3 9.60E-9 0.50
17 SGTC_2977 9011197 5.47 71.4 µM 9.55E-7 3 2.23E-8 0.65
18 SGTC_1516 st013856 5.47 110.0 µM 9.58E-7 3 2.24E-8 1.43
19 SGTC_2544 sphondin 5.45 68.5 µM 1.05E-6 RNA pol III & RNase P/MRP 21 2.53E-8 0.02
20 SGTC_580 1123-0028 5.38 41.0 µM 1.40E-6 DNA damage response 11 3.70E-8 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.461 2.07E-176 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.363 6.25E-105 YDR404C RPB7 het RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
0.359 1.99E-102 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.335 6.44E-89 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.311 4.23E-76 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.306 1.69E-73 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.300 1.06E-70 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.296 6.70E-69 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.283 6.08E-63 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.281 4.00E-62 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.280 1.97E-61 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.272 7.76E-58 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.267 5.06E-56 YLR321C SFH1 het Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
0.246 2.81E-47 YBR154C RPB5 het RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.240 4.55E-45 YMR033W ARP9 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation