YFL014W / HSP12

Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL014W / HSP12

Click on Significant Values for Screen Details ID:SGTC_144|Compound:4161-2749|FD-Score:-2.86|P-value:4.33E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-2.95|P-value:2.85E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.67|P-value:9.05E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:2.84|P-value:3.81E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.36|P-value:3.39E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-2.81|P-value:5.25E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.83|P-value:4.92E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:3.10|P-value:1.18E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:-2.84|P-value:4.67E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.89|P-value:3.81E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.07|P-value:1.36E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:-2.86|P-value:4.23E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.21|P-value:5.32E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.88|P-value:5.61E-9 ID:SGTC_1642|Compound:st009582|FD-Score:2.83|P-value:3.98E-4 ID:SGTC_1753|Compound:st043925|FD-Score:3.01|P-value:1.77E-4 ID:SGTC_1756|Compound:st041558|FD-Score:4.19|P-value:3.51E-7 ID:SGTC_1758|Compound:st042942|FD-Score:2.89|P-value:3.02E-4 ID:SGTC_1908|Compound:st060353|FD-Score:2.77|P-value:5.04E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-3.63|P-value:1.12E-5 ID:SGTC_1986|Compound:st071812|FD-Score:-2.81|P-value:5.22E-4 ID:SGTC_2014|Compound:4013930|FD-Score:4.37|P-value:1.22E-7 ID:SGTC_2330|Compound:7979373|FD-Score:-2.68|P-value:9.06E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.31|P-value:4.49E-5 ID:SGTC_2803|Compound:7707263|FD-Score:-3.98|P-value:1.64E-6 ID:SGTC_2829|Compound:7992743|FD-Score:2.78|P-value:4.86E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.48|P-value:2.44E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.55|P-value:1.71E-5 ID:SGTC_3292|Compound:9120825|FD-Score:2.77|P-value:5.12E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.86|P-value:4.33E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-2.95|P-value:2.85E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.67|P-value:9.05E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:2.84|P-value:3.81E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.36|P-value:3.39E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-2.81|P-value:5.25E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.83|P-value:4.92E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:3.10|P-value:1.18E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:-2.84|P-value:4.67E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.89|P-value:3.81E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.07|P-value:1.36E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:-2.86|P-value:4.23E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.21|P-value:5.32E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.88|P-value:5.61E-9 ID:SGTC_1642|Compound:st009582|FD-Score:2.83|P-value:3.98E-4 ID:SGTC_1753|Compound:st043925|FD-Score:3.01|P-value:1.77E-4 ID:SGTC_1756|Compound:st041558|FD-Score:4.19|P-value:3.51E-7 ID:SGTC_1758|Compound:st042942|FD-Score:2.89|P-value:3.02E-4 ID:SGTC_1908|Compound:st060353|FD-Score:2.77|P-value:5.04E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-3.63|P-value:1.12E-5 ID:SGTC_1986|Compound:st071812|FD-Score:-2.81|P-value:5.22E-4 ID:SGTC_2014|Compound:4013930|FD-Score:4.37|P-value:1.22E-7 ID:SGTC_2330|Compound:7979373|FD-Score:-2.68|P-value:9.06E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.31|P-value:4.49E-5 ID:SGTC_2803|Compound:7707263|FD-Score:-3.98|P-value:1.64E-6 ID:SGTC_2829|Compound:7992743|FD-Score:2.78|P-value:4.86E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.48|P-value:2.44E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.55|P-value:1.71E-5 ID:SGTC_3292|Compound:9120825|FD-Score:2.77|P-value:5.12E-4

Top fitness defect scores for YFL014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2014 4013930 4.37 200.0 µM 1.22E-7 19 6.31E-6
2 SGTC_1756 st041558 4.19 35.5 µM 3.51E-7 8 1.37E-5
3 SGTC_367 0335-0881 3.36 7.1 µM 3.39E-5 ubiquinone biosynthesis & proteasome 114 3.84E-4
4 SGTC_2799 7812892 3.31 81.8 µM 4.49E-5 superoxide 51 4.72E-4
5 SGTC_701 4424-0219 3.10 185.0 µM 1.18E-4 calcium & mitochondrial duress 47 9.57E-4
6 SGTC_987 1497-1013 3.07 404.0 µM 1.36E-4 42 0.00107
7 SGTC_1753 st043925 3.01 64.0 µM 1.77E-4 40 0.00129
8 SGTC_1758 st042942 2.89 75.2 µM 3.02E-4 60 0.00191
9 SGTC_287 k060-0039 2.84 29.6 µM 3.81E-4 47 0.00227
10 SGTC_1642 st009582 2.83 77.7 µM 3.98E-4 RPP1 & pyrimidine depletion 66 0.00234
11 SGTC_2829 7992743 2.78 58.4 µM 4.86E-4 114 0.00271
12 SGTC_1908 st060353 2.77 56.5 µM 5.04E-4 RPP1 & pyrimidine depletion 105 0.00278
13 SGTC_3292 9120825 2.77 59.8 µM 5.12E-4 65 0.00281
14 SGTC_798 1287-0182 2.57 476.0 µM 0.00113 115 0.00506
15 SGTC_2491 paeonol 2.57 100.0 µM 0.00116 57 0.00513
16 SGTC_3297 9117502 2.48 71.4 µM 0.00163 88 0.00660
17 SGTC_2483 5729050 2.48 27.2 µM 0.00165 147 0.00666
18 SGTC_933 2914-0560 2.43 114.0 µM 0.00198 136 0.00762
19 SGTC_1100 amiodarone 2.38 5.1 µM 0.00232 125 0.00856
20 SGTC_1703 st026983 2.35 48.3 µM 0.00263 129 0.00941

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.387 3.58E-120 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.380 1.62E-115 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.358 3.32E-102 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.353 5.53E-99 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.342 2.12E-92 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.338 1.13E-90 YDR497C ITR1 hom Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
0.333 1.73E-87 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.332 5.40E-87 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.330 2.06E-85 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.321 4.16E-81 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.304 1.10E-72 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.300 1.26E-70 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.283 5.78E-63 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.275 3.18E-59 YDR517W GRH1 hom Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
0.274 1.05E-58 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication