YFL023W / BUD27

Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL023W / BUD27

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:3.37|P-value:4.69E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.50|P-value:3.00E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:5.64|P-value:1.66E-8 ID:SGTC_57|Compound:miconazole|FD-Score:-3.52|P-value:3.02E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.68|P-value:1.66E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.24|P-value:7.36E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.34|P-value:5.29E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.53|P-value:2.70E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.44|P-value:1.65E-13 ID:SGTC_210|Compound:3551-0445|FD-Score:4.16|P-value:2.23E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.87|P-value:2.26E-22 ID:SGTC_232|Compound:clozapine|FD-Score:4.23|P-value:1.65E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:4.67|P-value:2.29E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:3.42|P-value:3.89E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.39|P-value:8.47E-6 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.16|P-value:2.10E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.44|P-value:3.98E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.52|P-value:2.79E-4 ID:SGTC_500|Compound:h-89|FD-Score:5.78|P-value:7.58E-9 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.43|P-value:6.92E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:-6.36|P-value:2.75E-10 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.82|P-value:9.96E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:3.62|P-value:1.90E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.65|P-value:1.73E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:4.85|P-value:9.92E-7 ID:SGTC_637|Compound:1181-0519|FD-Score:4.05|P-value:3.54E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:4.21|P-value:1.82E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.56|P-value:2.60E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:4.03|P-value:3.90E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.17|P-value:2.15E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:5.80|P-value:6.69E-9 ID:SGTC_834|Compound:0312-0008|FD-Score:3.16|P-value:9.75E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.46|P-value:3.42E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.45|P-value:3.54E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.64|P-value:1.81E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:4.99|P-value:4.96E-7 ID:SGTC_939|Compound:1165-0479|FD-Score:4.01|P-value:4.15E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:4.07|P-value:3.25E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.97|P-value:5.02E-5 ID:SGTC_982|Compound:1491-0518|FD-Score:3.64|P-value:1.78E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.10|P-value:2.88E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.95|P-value:6.23E-7 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.05|P-value:3.62E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.20|P-value:8.54E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:5.85|P-value:5.03E-9 ID:SGTC_1136|Compound:pergolide|FD-Score:3.70|P-value:1.43E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.57|P-value:3.70E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.03|P-value:3.83E-5 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.63|P-value:1.85E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:3.32|P-value:5.64E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.34|P-value:5.30E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.22|P-value:8.00E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.59|P-value:2.10E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.78|P-value:1.05E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.87|P-value:7.37E-5 ID:SGTC_1781|Compound:st038725|FD-Score:7.48|P-value:1.18E-13 ID:SGTC_1797|Compound:st048695|FD-Score:3.48|P-value:3.17E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.31|P-value:1.21E-5 ID:SGTC_1915|Compound:st067105|FD-Score:4.74|P-value:1.71E-6 ID:SGTC_1973|Compound:st070275|FD-Score:3.87|P-value:7.40E-5 ID:SGTC_1979|Compound:st071809|FD-Score:3.60|P-value:2.08E-4 ID:SGTC_1982|Compound:st072467|FD-Score:4.12|P-value:2.66E-5 ID:SGTC_1991|Compound:st073000|FD-Score:6.06|P-value:1.47E-9 ID:SGTC_2004|Compound:4023728|FD-Score:3.67|P-value:1.57E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.74|P-value:1.24E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.68|P-value:2.24E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.21|P-value:8.08E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.57|P-value:2.31E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.14|P-value:2.50E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.48|P-value:3.14E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.25|P-value:7.08E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.28|P-value:6.35E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.47|P-value:5.76E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.73|P-value:1.75E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.37|P-value:4.68E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.86|P-value:7.68E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.70|P-value:1.43E-4 ID:SGTC_2843|Compound:9000894|FD-Score:3.25|P-value:7.10E-4 ID:SGTC_2921|Compound:7974240|FD-Score:5.03|P-value:4.09E-7 ID:SGTC_3282|Compound:9108498|FD-Score:4.92|P-value:7.12E-7 ID:SGTC_3317|Compound:9138495|FD-Score:3.32|P-value:5.64E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:3.37|P-value:4.69E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.50|P-value:3.00E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:5.64|P-value:1.66E-8 ID:SGTC_57|Compound:miconazole|FD-Score:-3.52|P-value:3.02E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.68|P-value:1.66E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.24|P-value:7.36E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.34|P-value:5.29E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.53|P-value:2.70E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.44|P-value:1.65E-13 ID:SGTC_210|Compound:3551-0445|FD-Score:4.16|P-value:2.23E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.87|P-value:2.26E-22 ID:SGTC_232|Compound:clozapine|FD-Score:4.23|P-value:1.65E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:4.67|P-value:2.29E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:3.42|P-value:3.89E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.39|P-value:8.47E-6 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.16|P-value:2.10E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.44|P-value:3.98E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.52|P-value:2.79E-4 ID:SGTC_500|Compound:h-89|FD-Score:5.78|P-value:7.58E-9 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:4.43|P-value:6.92E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:-6.36|P-value:2.75E-10 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.82|P-value:9.96E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:3.62|P-value:1.90E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:3.65|P-value:1.73E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:4.85|P-value:9.92E-7 ID:SGTC_637|Compound:1181-0519|FD-Score:4.05|P-value:3.54E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:4.21|P-value:1.82E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.56|P-value:2.60E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:4.03|P-value:3.90E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.17|P-value:2.15E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:5.80|P-value:6.69E-9 ID:SGTC_834|Compound:0312-0008|FD-Score:3.16|P-value:9.75E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:3.46|P-value:3.42E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:3.45|P-value:3.54E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.64|P-value:1.81E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:4.99|P-value:4.96E-7 ID:SGTC_939|Compound:1165-0479|FD-Score:4.01|P-value:4.15E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:4.07|P-value:3.25E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.97|P-value:5.02E-5 ID:SGTC_982|Compound:1491-0518|FD-Score:3.64|P-value:1.78E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.10|P-value:2.88E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.95|P-value:6.23E-7 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.05|P-value:3.62E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.20|P-value:8.54E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:5.85|P-value:5.03E-9 ID:SGTC_1136|Compound:pergolide|FD-Score:3.70|P-value:1.43E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.57|P-value:3.70E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.03|P-value:3.83E-5 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.63|P-value:1.85E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:3.32|P-value:5.64E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.34|P-value:5.30E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.22|P-value:8.00E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.59|P-value:2.10E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.78|P-value:1.05E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.87|P-value:7.37E-5 ID:SGTC_1781|Compound:st038725|FD-Score:7.48|P-value:1.18E-13 ID:SGTC_1797|Compound:st048695|FD-Score:3.48|P-value:3.17E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.31|P-value:1.21E-5 ID:SGTC_1915|Compound:st067105|FD-Score:4.74|P-value:1.71E-6 ID:SGTC_1973|Compound:st070275|FD-Score:3.87|P-value:7.40E-5 ID:SGTC_1979|Compound:st071809|FD-Score:3.60|P-value:2.08E-4 ID:SGTC_1982|Compound:st072467|FD-Score:4.12|P-value:2.66E-5 ID:SGTC_1991|Compound:st073000|FD-Score:6.06|P-value:1.47E-9 ID:SGTC_2004|Compound:4023728|FD-Score:3.67|P-value:1.57E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.74|P-value:1.24E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.68|P-value:2.24E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.21|P-value:8.08E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.57|P-value:2.31E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.14|P-value:2.50E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.48|P-value:3.14E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.25|P-value:7.08E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.28|P-value:6.35E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.47|P-value:5.76E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.73|P-value:1.75E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.37|P-value:4.68E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.86|P-value:7.68E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.70|P-value:1.43E-4 ID:SGTC_2843|Compound:9000894|FD-Score:3.25|P-value:7.10E-4 ID:SGTC_2921|Compound:7974240|FD-Score:5.03|P-value:4.09E-7 ID:SGTC_3282|Compound:9108498|FD-Score:4.92|P-value:7.12E-7 ID:SGTC_3317|Compound:9138495|FD-Score:3.32|P-value:5.64E-4

Top fitness defect scores for YFL023W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 9.87 10.3 µM 2.26E-22 NEO1-PIK1 61 2.90E-23
2 SGTC_1781 st038725 7.48 66.3 µM 1.18E-13 NEO1 35 3.67E-14
3 SGTC_168 3970-0862 7.44 530.5 µM 1.65E-13 24 5.22E-14
4 SGTC_1991 st073000 6.06 8.9 µM 1.47E-9 NEO1 34 6.87E-10
5 SGTC_1112 tnp00069 5.85 1.2 µM 5.03E-9 4 2.49E-9
6 SGTC_832 4083-0019 5.80 164.0 µM 6.69E-9 translation 24 3.35E-9
7 SGTC_500 h-89 5.78 112.0 µM 7.58E-9 cell wall signaling 13 3.82E-9
8 SGTC_52 0329-0193 5.64 875.0 nM 1.66E-8 TSC3-RPN4 11 8.65E-9
9 SGTC_682 0kpi-0099 5.56 17.0 µM 2.60E-8 27 1.38E-8
10 SGTC_445 e6 berbamine 5.16 66.0 µM 2.10E-7 NEO1-PIK1 54 1.22E-7
11 SGTC_2921 7974240 5.03 10.7 µM 4.09E-7 26 2.44E-7
12 SGTC_921 1561-0023 4.99 372.0 µM 4.96E-7 7 2.98E-7
13 SGTC_1027 k015-0025 4.95 8.7 µM 6.23E-7 14 3.78E-7
14 SGTC_3282 9108498 4.92 15.5 µM 7.12E-7 16 4.34E-7
15 SGTC_535 2293-4129 4.85 48.2 µM 9.92E-7 NEO1 38 6.14E-7
16 SGTC_1915 st067105 4.74 30.6 µM 1.71E-6 RNA pol III & RNase P/MRP 23 1.08E-6
17 SGTC_2489 cantharidin 4.73 73.4 µM 1.75E-6 cell wall signaling 23 1.11E-6
18 SGTC_2060 5238662 4.68 140.0 µM 2.24E-6 15 1.44E-6
19 SGTC_301 4119-0100 4.67 24.5 µM 2.29E-6 NEO1 63 1.47E-6
20 SGTC_1239 0337-0584 4.57 35.4 µM 3.70E-6 unfolded protein response 37 2.42E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.385 8.35E-119 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.332 8.08E-87 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.326 1.37E-83 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.318 1.26E-79 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.317 3.05E-79 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.306 2.81E-73 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.295 5.86E-68 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.294 1.18E-67 YAL009W SPO7 hom Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.282 1.87E-62 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.278 1.81E-60 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.271 2.47E-57 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.265 7.57E-55 YGL072C_d YGL072C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.256 2.90E-51 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.250 1.03E-48 YDR378C LSM6 hom Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
0.249 1.79E-48 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion