YFL033C / RIM15

Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase

Zygosity: Homozygous strain
fixedexpanded
Profile for YFL033C / RIM15

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.97|P-value:1.93E-7 ID:SGTC_81|Compound:0411-0046|FD-Score:3.89|P-value:3.52E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:4.61|P-value:1.22E-6 ID:SGTC_114|Compound:0364-0001|FD-Score:4.04|P-value:1.80E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.53|P-value:8.37E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:3.66|P-value:9.01E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:4.04|P-value:1.86E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.15|P-value:6.27E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.38|P-value:3.91E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:13.00|P-value:1.86E-40 ID:SGTC_178|Compound:4092-0806|FD-Score:7.33|P-value:3.70E-14 ID:SGTC_196|Compound:k015-0023|FD-Score:3.75|P-value:6.32E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:8.33|P-value:9.47E-18 ID:SGTC_357|Compound:1325-0123|FD-Score:-3.24|P-value:4.99E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.05|P-value:9.80E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-13.00|P-value:4.36E-40 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.14|P-value:7.32E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.37|P-value:2.84E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.13|P-value:8.12E-8 ID:SGTC_529|Compound:4076-0302|FD-Score:3.44|P-value:2.22E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.30|P-value:3.73E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:4.06|P-value:1.69E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.49|P-value:1.81E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:4.01|P-value:2.08E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.11|P-value:8.13E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.50|P-value:2.19E-6 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.77|P-value:5.43E-7 ID:SGTC_1187|Compound:1391-0724|FD-Score:-3.67|P-value:9.75E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.74|P-value:7.21E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.17|P-value:6.03E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.43|P-value:2.25E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.21|P-value:8.34E-6 ID:SGTC_1520|Compound:flindersine|FD-Score:3.05|P-value:8.92E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.13|P-value:1.21E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.29|P-value:5.91E-6 ID:SGTC_1819|Compound:st053448|FD-Score:-3.26|P-value:4.74E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.36|P-value:2.99E-14 ID:SGTC_1906|Compound:st060206|FD-Score:3.23|P-value:4.71E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.56|P-value:1.35E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.35|P-value:3.08E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.34|P-value:5.01E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.30|P-value:4.79E-14 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.51|P-value:2.00E-18 ID:SGTC_2829|Compound:7992743|FD-Score:3.09|P-value:7.83E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.17|P-value:5.88E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.63|P-value:1.03E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.13|P-value:1.24E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:4.97|P-value:1.93E-7 ID:SGTC_81|Compound:0411-0046|FD-Score:3.89|P-value:3.52E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:4.61|P-value:1.22E-6 ID:SGTC_114|Compound:0364-0001|FD-Score:4.04|P-value:1.80E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.53|P-value:8.37E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:3.66|P-value:9.01E-5 ID:SGTC_150|Compound:3970-0969|FD-Score:4.04|P-value:1.86E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.15|P-value:6.27E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.38|P-value:3.91E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:13.00|P-value:1.86E-40 ID:SGTC_178|Compound:4092-0806|FD-Score:7.33|P-value:3.70E-14 ID:SGTC_196|Compound:k015-0023|FD-Score:3.75|P-value:6.32E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:8.33|P-value:9.47E-18 ID:SGTC_357|Compound:1325-0123|FD-Score:-3.24|P-value:4.99E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.05|P-value:9.80E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-13.00|P-value:4.36E-40 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.14|P-value:7.32E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.37|P-value:2.84E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.13|P-value:8.12E-8 ID:SGTC_529|Compound:4076-0302|FD-Score:3.44|P-value:2.22E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.30|P-value:3.73E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:4.06|P-value:1.69E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.49|P-value:1.81E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:4.01|P-value:2.08E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.11|P-value:8.13E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.50|P-value:2.19E-6 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.77|P-value:5.43E-7 ID:SGTC_1187|Compound:1391-0724|FD-Score:-3.67|P-value:9.75E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.74|P-value:7.21E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.17|P-value:6.03E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.43|P-value:2.25E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.21|P-value:8.34E-6 ID:SGTC_1520|Compound:flindersine|FD-Score:3.05|P-value:8.92E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.13|P-value:1.21E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.29|P-value:5.91E-6 ID:SGTC_1819|Compound:st053448|FD-Score:-3.26|P-value:4.74E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:7.36|P-value:2.99E-14 ID:SGTC_1906|Compound:st060206|FD-Score:3.23|P-value:4.71E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.56|P-value:1.35E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.35|P-value:3.08E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.34|P-value:5.01E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.30|P-value:4.79E-14 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.51|P-value:2.00E-18 ID:SGTC_2829|Compound:7992743|FD-Score:3.09|P-value:7.83E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.17|P-value:5.88E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.63|P-value:1.03E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.13|P-value:1.24E-5

Top fitness defect scores for YFL033C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_168 3970-0862 13.00 530.5 µM 1.86E-40 4 5.36E-39
2 SGTC_2688 kanamycin b 8.51 96.7 µM 2.00E-18 calcium & mitochondrial duress 51 8.83E-18
3 SGTC_214 4373-3496 8.33 70.0 µM 9.47E-18 3 3.96E-17
4 SGTC_1844 rapamycin 7.36 4.0 nM 2.99E-14 66 9.26E-14
5 SGTC_178 4092-0806 7.33 87.5 µM 3.70E-14 ergosterol depletion effects on membrane 23 1.14E-13
6 SGTC_2679 rapamycin 7.30 1.0 nM 4.79E-14 52 1.46E-13
7 SGTC_2250 alverine citrate 6.34 130.0 µM 5.01E-11 46 1.18E-10
8 SGTC_115 0527-0192 5.53 112.0 µM 8.37E-9 18 1.63E-8
9 SGTC_528 3492-0019 5.13 172.0 µM 8.12E-8 calcium & mitochondrial duress 149 1.45E-7
10 SGTC_43 0570-0105 4.97 72.5 µM 1.93E-7 ERG2 55 3.34E-7
11 SGTC_1183 1254-0324 4.77 198.0 µM 5.43E-7 10 9.04E-7
12 SGTC_108 0509-0257 4.61 621.1 µM 1.22E-6 32 1.97E-6
13 SGTC_1167 k781-0610 4.50 148.0 µM 2.19E-6 RNA processing & uracil transport 14 3.46E-6
14 SGTC_159 3970-0959 4.38 50.0 µM 3.91E-6 60 6.04E-6
15 SGTC_1712 st032288 4.29 53.4 µM 5.91E-6 calcium & mitochondrial duress 167 9.00E-6
16 SGTC_1340 1486-1359 4.21 257.0 µM 8.34E-6 15 1.25E-5
17 SGTC_1597 7-benzyloxygramine 4.13 47.5 µM 1.21E-5 calcium & mitochondrial duress 108 1.80E-5
18 SGTC_2896 9052656 4.13 71.4 µM 1.24E-5 endomembrane recycling 101 1.83E-5
19 SGTC_922 1866-0035 4.06 480.0 µM 1.69E-5 excess fatty acid 53 2.47E-5
20 SGTC_114 0364-0001 4.04 490.3 µM 1.80E-5 47 2.63E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.450 4.25E-167 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.312 9.71E-77 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
0.260 7.29E-53 YOR043W WHI2 hom Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression
0.256 2.53E-51 YOR132W VPS17 hom Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
0.252 1.63E-49 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.251 1.88E-49 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.248 2.69E-48 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.247 6.06E-48 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
0.247 1.00E-47 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.246 2.20E-47 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.235 4.07E-43 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.231 1.07E-41 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.226 3.85E-40 YBR111W-A SUS1 hom Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
0.223 3.80E-39 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.222 1.20E-38 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant