HIPHOP chemogenomics database

YFR004W / RPN11

Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YFR004W / RPN11

Click on Significant Values for Screen Details

Top fitness defect scores for YFR004W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2124	5246320	7.56	200.0 µM	1.37E-12	heme biosynthesis & mitochondrial translocase	12	2.09E-14	1.23
2	SGTC_2004	4023728	7.13	41.9 µM	2.14E-11		12	5.17E-13	0.22
3	SGTC_3142	9086498	6.96	49.5 µM	6.04E-11	iron homeostasis	19	1.74E-12	0.34
4	SGTC_1016	4226-1401	6.75	38.0 µM	2.10E-10	RNA pol III & RNase P/MRP	6	7.42E-12	0.64
5	SGTC_2519	cryptotanshinone	5.99	88.0 µM	1.49E-8	heme biosynthesis & mitochondrial translocase	8	1.07E-9	0.42
6	SGTC_3015	9080845	4.92	49.5 µM	2.61E-6		2	4.40E-7	0.72
7	SGTC_1131	3454-3128	4.74	32.3 µM	5.54E-6		7	1.06E-6	0.09
8	SGTC_2399	5884133	4.71	200.0 µM	6.33E-6	cell wall signaling	11	1.23E-6	0.14
9	SGTC_1716	st033144	4.61	30.6 µM	9.77E-6		5	2.04E-6	0.48
10	SGTC_359	0081-0058	4.59	15.9 µM	1.07E-5	60S ribosome export	9	2.26E-6	0.27
11	SGTC_2348	9022543	4.57	33.4 µM	1.14E-5	mitochondrial processes	25	2.44E-6	0.02
12	SGTC_744	1594-0068	4.51	283.0 µM	1.48E-5	amide catabolism	2	3.32E-6	0.15
13	SGTC_1918	oxolan-2-ylmethyl 3-bromobenzoate	4.49	70.1 µM	1.58E-5		2	3.58E-6	0.36
14	SGTC_2790	5423970	4.39	71.4 µM	2.33E-5	endomembrane recycling	13	5.61E-6	0.06
15	SGTC_1809	st050081	4.31	57.6 µM	3.16E-5		5	7.99E-6	0.45
16	SGTC_2310	7668677	4.27	200.0 µM	3.78E-5		4	9.83E-6	0.17
17	SGTC_942	3253-1115	4.19	227.0 µM	5.15E-5		4	1.41E-5	0.30
18	SGTC_2129	girgensonine	4.16	121.0 µM	5.65E-5	heme biosynthesis & mitochondrial translocase	20	1.57E-5	0.03
19	SGTC_2538	3',7'-epoxycaryophyllan-6-one	4.09	100.0 µM	7.55E-5		9	2.19E-5	0.02
20	SGTC_2306	7482397	4.05	179.5 µM	8.67E-5	RPP1 & pyrimidine depletion	10	2.57E-5	0.29

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.282	4.84E-62	YOR117W	RPT5	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.236	1.29E-43	YER021W	RPN3	het	Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.228	1.01E-40	YDL007W	RPT2	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.224	4.33E-39	YDL097C	RPN6	het	Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.219	1.11E-37	YGL048C	RPT6	het	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.195	6.20E-30	YDR427W	RPN9	het	Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.193	1.74E-29	YFR052W	RPN12	het	Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.192	4.56E-29	YDL147W	RPN5	het	Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.145	3.53E-17	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.143	8.61E-17	YOR261C	RPN8	het	Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
0.142	1.92E-16	YDR527W	RBA50	het	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.141	3.24E-16	YDR394W	RPT3	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.136	2.79E-15	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.126	2.67E-13	YOR259C	RPT4	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.115	2.24E-11	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress