YFR042W / KEG1

Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR042W / KEG1

Click on Significant Values for Screen Details ID:SGTC_148|Compound:3253-0744|FD-Score:-2.96|P-value:9.85E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.41|P-value:1.53E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:-3.05|P-value:7.21E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.52|P-value:8.79E-7 ID:SGTC_701|Compound:4424-0219|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.88|P-value:1.98E-5 ID:SGTC_854|Compound:0422-0053|FD-Score:-3.01|P-value:8.34E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.06|P-value:8.66E-6 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.58|P-value:7.58E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.97|P-value:1.28E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.28|P-value:3.73E-6 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-4.02|P-value:1.30E-5 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.35|P-value:1.89E-4 ID:SGTC_1704|Compound:st028805|FD-Score:-3.13|P-value:5.36E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.28|P-value:2.50E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-3.01|P-value:8.42E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-7.23|P-value:1.59E-14 ID:SGTC_2054|Compound:5233173|FD-Score:-5.50|P-value:4.04E-9 ID:SGTC_2055|Compound:5235184|FD-Score:2.96|P-value:8.58E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.14|P-value:4.38E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.21|P-value:3.88E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:-3.09|P-value:6.18E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.51|P-value:9.81E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.01|P-value:8.34E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.00|P-value:8.52E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.01|P-value:8.18E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-4.43|P-value:1.72E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-7.75|P-value:2.03E-16 ID:SGTC_3325|Compound:9138760|FD-Score:3.21|P-value:3.38E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-2.96|P-value:9.85E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.41|P-value:1.53E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:-3.05|P-value:7.21E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.52|P-value:8.79E-7 ID:SGTC_701|Compound:4424-0219|FD-Score:3.35|P-value:1.90E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.88|P-value:1.98E-5 ID:SGTC_854|Compound:0422-0053|FD-Score:-3.01|P-value:8.34E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.06|P-value:8.66E-6 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.58|P-value:7.58E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.97|P-value:1.28E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.28|P-value:3.73E-6 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-4.02|P-value:1.30E-5 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.35|P-value:1.89E-4 ID:SGTC_1704|Compound:st028805|FD-Score:-3.13|P-value:5.36E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.28|P-value:2.50E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-3.01|P-value:8.42E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-7.23|P-value:1.59E-14 ID:SGTC_2054|Compound:5233173|FD-Score:-5.50|P-value:4.04E-9 ID:SGTC_2055|Compound:5235184|FD-Score:2.96|P-value:8.58E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.14|P-value:4.38E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.21|P-value:3.88E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:-3.09|P-value:6.18E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.51|P-value:9.81E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.01|P-value:8.34E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.00|P-value:8.52E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.01|P-value:8.18E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-4.43|P-value:1.72E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-7.75|P-value:2.03E-16 ID:SGTC_3325|Compound:9138760|FD-Score:3.21|P-value:3.38E-4

Top fitness defect scores for YFR042W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_629 3448-6466 4.52 76.7 µM 8.79E-7 2 3.15E-6 0.85
2 SGTC_1172 0370-0043 4.06 85.0 µM 8.66E-6 2 2.47E-5 0.08
3 SGTC_1378 2093-0007 3.97 380.0 nM 1.28E-5 5 3.52E-5 0.11
4 SGTC_705 1124-0212 3.88 310.0 µM 1.98E-5 8 5.22E-5 0.01
5 SGTC_1370 2001-0092 3.58 44.6 µM 7.58E-5 8 1.75E-4 0.05
6 SGTC_2922 7999257 3.51 32.5 µM 9.81E-5 9 2.21E-4 0.03
7 SGTC_534 k292-0788 3.41 63.4 µM 1.53E-4 mitochondrial processes 16 3.29E-4 0.23
8 SGTC_1680 diphenhydramine 3.35 100.0 µM 1.89E-4 NEO1 20 3.99E-4 0.19
9 SGTC_701 4424-0219 3.35 185.0 µM 1.90E-4 calcium & mitochondrial duress 4 4.02E-4 0.04
10 SGTC_1746 st038117 3.28 42.0 µM 2.50E-4 10 5.13E-4 0.11
11 SGTC_3325 9138760 3.21 55.2 µM 3.38E-4 Golgi 10 6.74E-4 0.01
12 SGTC_2411 st077133 3.14 39.9 µM 4.38E-4 RNA pol III & RNase P/MRP 34 8.52E-4 0.02
13 SGTC_2055 5235184 2.96 200.0 µM 8.58E-4 ERG2 13 0.00156 0.06
14 SGTC_2975 9084847 2.88 53.7 µM 0.00110 23 0.00196 0.05
15 SGTC_1007 4101-0031 2.88 70.0 µM 0.00111 14 0.00197 0.07
16 SGTC_1077 5-fluorocytosine 2.88 377.0 nM 0.00111 exosome 30 0.00197 0.09
17 SGTC_2723 salbutamol 2.80 83.6 µM 0.00149 4 0.00257 5.55E-5
18 SGTC_821 1114-0266 2.75 29.2 µM 0.00176 16 0.00300 0.01
19 SGTC_2945 9067681 2.74 61.8 µM 0.00180 23 0.00305 0.03
20 SGTC_3063 1327-0034 2.73 4.0 µM 0.00187 20 0.00315 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.280 1.27E-61 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.248 4.81E-48 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed
0.164 1.33E-21 YFL041W FET5 hom Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport
0.159 2.54E-20 YDR434W GPI17 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
0.146 1.53E-17 YDR037W KRS1 het Lysyl-tRNA synthetase
0.144 4.81E-17 YDR013W PSF1 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.143 9.27E-17 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.142 1.59E-16 YKR094C RPL40B hom Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
0.140 4.24E-16 YJR078W BNA2 hom Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp
0.132 1.42E-14 YGR069W_d YGR069W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.127 1.91E-13 YDL008W APC11 het Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
0.125 3.35E-13 YJR135C MCM22 hom Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
0.124 6.53E-13 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.122 1.28E-12 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.119 4.90E-12 YEL056W HAT2 hom Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing