YFR052W / RPN12

Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFR052W / RPN12

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:-3.19|P-value:3.69E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.06|P-value:4.65E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.93|P-value:7.77E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.16|P-value:4.01E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:4.55|P-value:4.82E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:2.95|P-value:7.15E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:2.95|P-value:7.16E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:2.92|P-value:7.81E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.48|P-value:6.96E-7 ID:SGTC_359|Compound:0081-0058|FD-Score:4.04|P-value:6.74E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-2.96|P-value:8.71E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:3.28|P-value:2.01E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.89|P-value:1.35E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.89|P-value:1.35E-5 ID:SGTC_497|Compound:capsazepine|FD-Score:3.65|P-value:4.08E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.22|P-value:2.47E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.52|P-value:7.97E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.08|P-value:4.29E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.37|P-value:1.79E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.57|P-value:4.19E-7 ID:SGTC_1190|Compound:1486-1332|FD-Score:-4.25|P-value:3.24E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.93|P-value:1.48E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:4.70|P-value:2.08E-7 ID:SGTC_1286|Compound:0929-0070|FD-Score:-3.11|P-value:4.89E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.02|P-value:9.76E-6 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.19|P-value:3.61E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.22|P-value:3.16E-4 ID:SGTC_1320|Compound:1313-0481|FD-Score:3.39|P-value:1.26E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.12|P-value:3.72E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.96|P-value:6.09E-14 ID:SGTC_1351|Compound:1496-0003|FD-Score:5.33|P-value:4.88E-9 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.42|P-value:1.46E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.10|P-value:5.16E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.04|P-value:6.52E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.02|P-value:7.01E-4 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.58|P-value:5.64E-5 ID:SGTC_1612|Compound:st002233|FD-Score:-3.14|P-value:4.37E-4 ID:SGTC_1695|Compound:st026914|FD-Score:2.99|P-value:6.10E-4 ID:SGTC_1763|Compound:st044482|FD-Score:-2.97|P-value:8.16E-4 ID:SGTC_1767|Compound:st044992|FD-Score:-3.72|P-value:3.94E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.02|P-value:5.56E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.14|P-value:3.41E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.83|P-value:1.77E-5 ID:SGTC_2004|Compound:4023728|FD-Score:3.91|P-value:1.21E-5 ID:SGTC_2016|Compound:5121758|FD-Score:5.70|P-value:4.31E-10 ID:SGTC_2019|Compound:4016004|FD-Score:3.81|P-value:1.93E-5 ID:SGTC_2072|Compound:5231554|FD-Score:-3.00|P-value:7.47E-4 ID:SGTC_2124|Compound:5246320|FD-Score:9.74|P-value:6.76E-26 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.96|P-value:9.68E-6 ID:SGTC_2241|Compound:6772625|FD-Score:-2.97|P-value:8.34E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-3.04|P-value:6.42E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.39|P-value:1.25E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.03|P-value:5.18E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-2.94|P-value:9.35E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.14|P-value:3.45E-4 ID:SGTC_2460|Compound:5552655|FD-Score:2.95|P-value:7.07E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.33|P-value:2.08E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.23|P-value:2.41E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.27|P-value:2.02E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.30|P-value:2.30E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.19|P-value:3.64E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.14|P-value:4.47E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-3.09|P-value:5.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-2.99|P-value:7.82E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.33|P-value:1.63E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.37|P-value:1.37E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-2.95|P-value:9.02E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.19|P-value:3.69E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.06|P-value:4.65E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.93|P-value:7.77E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.16|P-value:4.01E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:4.55|P-value:4.82E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:2.95|P-value:7.15E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:2.95|P-value:7.16E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:2.92|P-value:7.81E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.48|P-value:6.96E-7 ID:SGTC_359|Compound:0081-0058|FD-Score:4.04|P-value:6.74E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-2.96|P-value:8.71E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:3.28|P-value:2.01E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.89|P-value:1.35E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.89|P-value:1.35E-5 ID:SGTC_497|Compound:capsazepine|FD-Score:3.65|P-value:4.08E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.22|P-value:2.47E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.52|P-value:7.97E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.08|P-value:4.29E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.37|P-value:1.79E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:4.57|P-value:4.19E-7 ID:SGTC_1190|Compound:1486-1332|FD-Score:-4.25|P-value:3.24E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.93|P-value:1.48E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:4.70|P-value:2.08E-7 ID:SGTC_1286|Compound:0929-0070|FD-Score:-3.11|P-value:4.89E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.02|P-value:9.76E-6 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.19|P-value:3.61E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.22|P-value:3.16E-4 ID:SGTC_1320|Compound:1313-0481|FD-Score:3.39|P-value:1.26E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.12|P-value:3.72E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-6.96|P-value:6.09E-14 ID:SGTC_1351|Compound:1496-0003|FD-Score:5.33|P-value:4.88E-9 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.42|P-value:1.46E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.10|P-value:5.16E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.04|P-value:6.52E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.02|P-value:7.01E-4 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.58|P-value:5.64E-5 ID:SGTC_1612|Compound:st002233|FD-Score:-3.14|P-value:4.37E-4 ID:SGTC_1695|Compound:st026914|FD-Score:2.99|P-value:6.10E-4 ID:SGTC_1763|Compound:st044482|FD-Score:-2.97|P-value:8.16E-4 ID:SGTC_1767|Compound:st044992|FD-Score:-3.72|P-value:3.94E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.02|P-value:5.56E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.14|P-value:3.41E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.83|P-value:1.77E-5 ID:SGTC_2004|Compound:4023728|FD-Score:3.91|P-value:1.21E-5 ID:SGTC_2016|Compound:5121758|FD-Score:5.70|P-value:4.31E-10 ID:SGTC_2019|Compound:4016004|FD-Score:3.81|P-value:1.93E-5 ID:SGTC_2072|Compound:5231554|FD-Score:-3.00|P-value:7.47E-4 ID:SGTC_2124|Compound:5246320|FD-Score:9.74|P-value:6.76E-26 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.96|P-value:9.68E-6 ID:SGTC_2241|Compound:6772625|FD-Score:-2.97|P-value:8.34E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-3.04|P-value:6.42E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.39|P-value:1.25E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.03|P-value:5.18E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-2.94|P-value:9.35E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.14|P-value:3.45E-4 ID:SGTC_2460|Compound:5552655|FD-Score:2.95|P-value:7.07E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.33|P-value:2.08E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.23|P-value:2.41E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.27|P-value:2.02E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.30|P-value:2.30E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.19|P-value:3.64E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.14|P-value:4.47E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-3.09|P-value:5.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-2.99|P-value:7.82E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.33|P-value:1.63E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.37|P-value:1.37E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-2.95|P-value:9.02E-4

Top fitness defect scores for YFR052W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2124 5246320 9.74 200.0 µM 6.76E-26 heme biosynthesis & mitochondrial translocase 8 1.01E-22 0.57
2 SGTC_2016 5121758 5.70 140.0 µM 4.31E-10 redox potentiating 7 6.00E-9 0.53
3 SGTC_1351 1496-0003 5.33 46.5 µM 4.88E-9 1 4.97E-8 0.76
4 SGTC_1200 1643-0193 4.70 18.4 µM 2.08E-7 3 1.31E-6 0.72
5 SGTC_1132 3456-3614 4.57 2.5 µM 4.19E-7 tubulin folding & SWR complex 5 2.41E-6 0.04
6 SGTC_179 1273-0063 4.55 6.8 µM 4.82E-7 3 2.73E-6 0.45
7 SGTC_356 1634-0012 4.48 32.5 µM 6.96E-7 6 3.75E-6 0.13
8 SGTC_359 0081-0058 4.04 15.9 µM 6.74E-6 60S ribosome export 14 2.72E-5 0.13
9 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 3.96 49.5 µM 9.68E-6 23 3.73E-5 0.17
10 SGTC_2004 4023728 3.91 41.9 µM 1.21E-5 27 4.53E-5 0.13
11 SGTC_416 estradiol valerate 3.89 100.0 µM 1.35E-5 4 4.99E-5 0.02
12 SGTC_462 ag-879 3.89 158.0 µM 1.35E-5 14 5.00E-5 0.08
13 SGTC_1967 st070177 3.83 51.4 µM 1.77E-5 6 6.31E-5 0.06
14 SGTC_2019 4016004 3.81 108.0 µM 1.93E-5 9 6.82E-5 0.16
15 SGTC_497 capsazepine 3.65 133.0 µM 4.08E-5 5 1.31E-4 0.06
16 SGTC_1552 6-methylflavone 3.58 10.6 µM 5.64E-5 superoxide 17 1.74E-4 0.30
17 SGTC_2289 7946404 3.39 165.1 µM 1.25E-4 27 3.47E-4 0.10
18 SGTC_1320 1313-0481 3.39 95.3 µM 1.26E-4 6 3.50E-4 0.15
19 SGTC_3157 9099274 3.37 49.5 µM 1.37E-4 8 3.78E-4 0.06
20 SGTC_3142 9086498 3.33 49.5 µM 1.63E-4 iron homeostasis 57 4.40E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.224 2.55E-39 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.208 3.79E-34 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.193 1.74E-29 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.193 1.52E-29 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.174 4.01E-24 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.169 6.62E-23 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.161 6.47E-21 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.160 1.43E-20 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.134 7.91E-15 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.132 1.75E-14 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.130 4.41E-14 YKL183W LOT5 hom Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
0.126 2.28E-13 YBR263W SHM1 hom Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
0.125 4.04E-13 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.118 7.13E-12 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.102 3.35E-9 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)