YFR053C / HXK1

Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR053C / HXK1

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:3.35|P-value:7.26E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:4.02|P-value:6.33E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-4.19|P-value:2.34E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:7.37|P-value:8.70E-13 ID:SGTC_393|Compound:acivicin|FD-Score:6.53|P-value:2.03E-10 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.67|P-value:2.37E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.62|P-value:5.24E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:3.26|P-value:9.64E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:4.21|P-value:2.93E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:3.58|P-value:3.22E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.56|P-value:2.67E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:3.78|P-value:1.61E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:4.45|P-value:1.10E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.25|P-value:7.59E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.54|P-value:3.73E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.33|P-value:5.98E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.91|P-value:1.40E-6 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.04|P-value:5.78E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.70|P-value:2.46E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:6.58|P-value:1.52E-10 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.55|P-value:3.63E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.97|P-value:7.78E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.92|P-value:9.36E-5 ID:SGTC_1749|Compound:st037932|FD-Score:5.46|P-value:9.11E-8 ID:SGTC_1775|Compound:st081588|FD-Score:3.84|P-value:1.29E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.95|P-value:8.25E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.62|P-value:2.87E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.57|P-value:3.35E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:4.19|P-value:3.25E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:5.04|P-value:7.28E-7 ID:SGTC_2117|Compound:5521686|FD-Score:4.81|P-value:2.19E-6 ID:SGTC_2211|Compound:7117706|FD-Score:3.54|P-value:3.80E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:-3.85|P-value:9.01E-5 ID:SGTC_2285|Compound:7945142|FD-Score:3.70|P-value:2.10E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.73|P-value:1.88E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.21|P-value:8.73E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.53|P-value:3.90E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.44|P-value:5.35E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.36|P-value:7.02E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.53|P-value:3.85E-4 ID:SGTC_3307|Compound:9126885|FD-Score:4.95|P-value:1.12E-6 ID:SGTC_3313|Compound:9133968|FD-Score:-3.49|P-value:3.35E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.78|P-value:1.57E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:3.35|P-value:7.26E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:4.02|P-value:6.33E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-4.19|P-value:2.34E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:7.37|P-value:8.70E-13 ID:SGTC_393|Compound:acivicin|FD-Score:6.53|P-value:2.03E-10 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.67|P-value:2.37E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.62|P-value:5.24E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:3.26|P-value:9.64E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:4.21|P-value:2.93E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:3.58|P-value:3.22E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.56|P-value:2.67E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:3.78|P-value:1.61E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:4.45|P-value:1.10E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.25|P-value:7.59E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.54|P-value:3.73E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.33|P-value:5.98E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.91|P-value:1.40E-6 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.04|P-value:5.78E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.70|P-value:2.46E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:6.58|P-value:1.52E-10 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.55|P-value:3.63E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.97|P-value:7.78E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.92|P-value:9.36E-5 ID:SGTC_1749|Compound:st037932|FD-Score:5.46|P-value:9.11E-8 ID:SGTC_1775|Compound:st081588|FD-Score:3.84|P-value:1.29E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.95|P-value:8.25E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.62|P-value:2.87E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.57|P-value:3.35E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:4.19|P-value:3.25E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:5.04|P-value:7.28E-7 ID:SGTC_2117|Compound:5521686|FD-Score:4.81|P-value:2.19E-6 ID:SGTC_2211|Compound:7117706|FD-Score:3.54|P-value:3.80E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:-3.85|P-value:9.01E-5 ID:SGTC_2285|Compound:7945142|FD-Score:3.70|P-value:2.10E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.73|P-value:1.88E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.21|P-value:8.73E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.53|P-value:3.90E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.44|P-value:5.35E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.36|P-value:7.02E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.53|P-value:3.85E-4 ID:SGTC_3307|Compound:9126885|FD-Score:4.95|P-value:1.12E-6 ID:SGTC_3313|Compound:9133968|FD-Score:-3.49|P-value:3.35E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.78|P-value:1.57E-4

Top fitness defect scores for YFR053C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_392 N-ethylmaleimide 7.37 8.3 µM 8.70E-13 RNA pol III & RNase P/MRP 1 8.71E-14
2 SGTC_1135 dihydrocapsaicin 6.58 536.7 nM 1.52E-10 4 2.34E-11
3 SGTC_393 acivicin 6.53 6.2 µM 2.03E-10 5 3.20E-11
4 SGTC_1749 st037932 5.46 54.2 µM 9.11E-8 12 2.40E-8
5 SGTC_1951 sophoraflavanone G 5.04 9.2 µM 7.28E-7 RNA processing & uracil transport 5 2.28E-7
6 SGTC_3307 9126885 4.95 69.3 µM 1.12E-6 17 3.63E-7
7 SGTC_1005 4092-0690 4.91 4.4 µM 1.40E-6 plasma membrane duress 16 4.64E-7
8 SGTC_2117 5521686 4.81 200.0 µM 2.19E-6 6 7.52E-7
9 SGTC_464 bw-b 70c 4.62 158.0 µM 5.24E-6 10 1.94E-6
10 SGTC_907 3448-6986 4.45 75.5 µM 1.10E-5 16 4.33E-6
11 SGTC_657 3534-0529 4.21 86.7 µM 2.93E-5 10 1.25E-5
12 SGTC_1928 4'-methoxychalcone 4.19 40.3 µM 3.25E-5 7 1.40E-5
13 SGTC_1024 4507-0395 4.04 26.0 µM 5.78E-5 TSC3-RPN4 24 2.62E-5
14 SGTC_382 3229-0966 4.02 23.2 µM 6.33E-5 18 2.89E-5
15 SGTC_1366 1683-0113 3.97 21.9 µM 7.78E-5 12 3.61E-5
16 SGTC_1791 st048313 3.95 56.1 µM 8.25E-5 15 3.85E-5
17 SGTC_1685 st024049 3.92 53.1 µM 9.36E-5 TSC3-RPN4 15 4.42E-5
18 SGTC_1775 st081588 3.84 35.2 µM 1.29E-4 60S ribosome export 25 6.27E-5
19 SGTC_3316 9136639 3.78 10.6 µM 1.57E-4 16 7.76E-5
20 SGTC_881 0833-0569 3.78 170.0 µM 1.61E-4 39 7.96E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 7.47E-18 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.108 3.88E-10 YMR254C_d YMR254C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.100 6.86E-9 YJR103W URA8 hom Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
0.087 4.62E-7 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.082 2.16E-6 YFR046C CNN1 hom Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
0.081 2.52E-6 YOR287C RRP36 het Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
0.076 1.06E-5 YMR049C ERB1 het Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
0.073 2.48E-5 YEL072W RMD6 hom Protein required for sporulation
0.069 6.94E-5 YMR261C TPS3 hom Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
0.066 1.34E-4 YPL081W RPS9A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
0.066 1.43E-4 YNR056C BIO5 hom Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
0.066 1.44E-4 YEL041W YEF1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
0.065 1.58E-4 YHR015W MIP6 hom Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.065 1.75E-4 YLR303W MET17 hom Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis
0.064 2.06E-4 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis