YGL040C / HEM2

Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL040C / HEM2

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.51|P-value:2.57E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.71|P-value:1.24E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-5.15|P-value:1.49E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:-5.91|P-value:2.13E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.57|P-value:2.77E-6 ID:SGTC_208|Compound:k072-0098|FD-Score:11.00|P-value:6.04E-28 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.82|P-value:8.03E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.67|P-value:1.87E-6 ID:SGTC_406|Compound:estrone|FD-Score:-3.78|P-value:8.30E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.85|P-value:6.91E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.07|P-value:2.85E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.36|P-value:4.07E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.04|P-value:3.21E-5 ID:SGTC_543|Compound:3844-1095|FD-Score:3.45|P-value:3.28E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:5.32|P-value:6.96E-8 ID:SGTC_562|Compound:3772-2733|FD-Score:4.51|P-value:4.00E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.24|P-value:6.22E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:6.24|P-value:3.30E-10 ID:SGTC_634|Compound:1611-4317|FD-Score:3.28|P-value:5.90E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.93|P-value:5.01E-5 ID:SGTC_842|Compound:0271-0008|FD-Score:3.44|P-value:3.40E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:3.15|P-value:9.36E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.40|P-value:3.82E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.59|P-value:1.96E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:27.50|P-value:4.54E-164 ID:SGTC_998|Compound:3996-0085|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-4.43|P-value:5.27E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.37|P-value:4.39E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:6.03|P-value:1.22E-9 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.42|P-value:3.28E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:5.39|P-value:4.87E-8 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.21|P-value:1.57E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.13|P-value:9.11E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.94|P-value:5.10E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:-3.60|P-value:1.66E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:3.44|P-value:3.36E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.85|P-value:7.15E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.39|P-value:4.09E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:4.36|P-value:7.95E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.59|P-value:1.96E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:4.34|P-value:8.79E-6 ID:SGTC_1556|Compound:plumbagin|FD-Score:5.17|P-value:1.56E-7 ID:SGTC_1701|Compound:st028638|FD-Score:3.91|P-value:5.44E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.16|P-value:8.34E-4 ID:SGTC_1798|Compound:st045719|FD-Score:3.81|P-value:8.36E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.48|P-value:2.86E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.80|P-value:8.59E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:3.25|P-value:6.59E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.94|P-value:4.49E-7 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:2.16E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.34|P-value:4.36E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.20|P-value:7.90E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.65|P-value:1.41E-4 ID:SGTC_2285|Compound:7945142|FD-Score:3.62|P-value:1.72E-4 ID:SGTC_2336|Compound:9001819|FD-Score:5.77|P-value:5.55E-9 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.15|P-value:2.03E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.98|P-value:4.12E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.66|P-value:1.46E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:9.01|P-value:2.13E-19 ID:SGTC_2575|Compound:plumbagin|FD-Score:7.82|P-value:4.87E-15 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.27|P-value:6.18E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:4.32|P-value:9.47E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:5.90|P-value:2.67E-9 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.76|P-value:1.22E-6 ID:SGTC_2784|Compound:7567673|FD-Score:3.65|P-value:1.52E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.56|P-value:2.15E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.50|P-value:4.20E-6 ID:SGTC_2936|Compound:9038235|FD-Score:-3.14|P-value:8.82E-4 ID:SGTC_2963|Compound:9082507|FD-Score:4.14|P-value:2.11E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.32|P-value:5.09E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.51|P-value:2.57E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.71|P-value:1.24E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-5.15|P-value:1.49E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:-5.91|P-value:2.13E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.57|P-value:2.77E-6 ID:SGTC_208|Compound:k072-0098|FD-Score:11.00|P-value:6.04E-28 ID:SGTC_217|Compound:ketoconazole|FD-Score:3.82|P-value:8.03E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.67|P-value:1.87E-6 ID:SGTC_406|Compound:estrone|FD-Score:-3.78|P-value:8.30E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.85|P-value:6.91E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.07|P-value:2.85E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.36|P-value:4.07E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.04|P-value:3.21E-5 ID:SGTC_543|Compound:3844-1095|FD-Score:3.45|P-value:3.28E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:5.32|P-value:6.96E-8 ID:SGTC_562|Compound:3772-2733|FD-Score:4.51|P-value:4.00E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.24|P-value:6.22E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:6.24|P-value:3.30E-10 ID:SGTC_634|Compound:1611-4317|FD-Score:3.28|P-value:5.90E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.93|P-value:5.01E-5 ID:SGTC_842|Compound:0271-0008|FD-Score:3.44|P-value:3.40E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:3.15|P-value:9.36E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.40|P-value:3.82E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.59|P-value:1.96E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:27.50|P-value:4.54E-164 ID:SGTC_998|Compound:3996-0085|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-4.43|P-value:5.27E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.37|P-value:4.39E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:6.03|P-value:1.22E-9 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.42|P-value:3.28E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:5.39|P-value:4.87E-8 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.21|P-value:1.57E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.13|P-value:9.11E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.94|P-value:5.10E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:-3.60|P-value:1.66E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:3.44|P-value:3.36E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.85|P-value:7.15E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.39|P-value:4.09E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:4.36|P-value:7.95E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.59|P-value:1.96E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:4.34|P-value:8.79E-6 ID:SGTC_1556|Compound:plumbagin|FD-Score:5.17|P-value:1.56E-7 ID:SGTC_1701|Compound:st028638|FD-Score:3.91|P-value:5.44E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.16|P-value:8.34E-4 ID:SGTC_1798|Compound:st045719|FD-Score:3.81|P-value:8.36E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.48|P-value:2.86E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.80|P-value:8.59E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:3.25|P-value:6.59E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.94|P-value:4.49E-7 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:2.16E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.34|P-value:4.36E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.20|P-value:7.90E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.65|P-value:1.41E-4 ID:SGTC_2285|Compound:7945142|FD-Score:3.62|P-value:1.72E-4 ID:SGTC_2336|Compound:9001819|FD-Score:5.77|P-value:5.55E-9 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.15|P-value:2.03E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.98|P-value:4.12E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.66|P-value:1.46E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:9.01|P-value:2.13E-19 ID:SGTC_2575|Compound:plumbagin|FD-Score:7.82|P-value:4.87E-15 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.27|P-value:6.18E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:4.32|P-value:9.47E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:5.90|P-value:2.67E-9 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.76|P-value:1.22E-6 ID:SGTC_2784|Compound:7567673|FD-Score:3.65|P-value:1.52E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.56|P-value:2.15E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.50|P-value:4.20E-6 ID:SGTC_2936|Compound:9038235|FD-Score:-3.14|P-value:8.82E-4 ID:SGTC_2963|Compound:9082507|FD-Score:4.14|P-value:2.11E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.32|P-value:5.09E-4

Top fitness defect scores for YGL040C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_996 3970-0799 27.50 1.8 µM 4.54E-164 1 8.03E-167 18.30
2 SGTC_208 k072-0098 11.00 12.4 µM 6.04E-28 heme biosynthesis & mitochondrial translocase 4 2.01E-28 2.20
3 SGTC_2519 cryptotanshinone 9.01 88.0 µM 2.13E-19 heme biosynthesis & mitochondrial translocase 5 9.92E-20 2.23
4 SGTC_2575 plumbagin 7.82 510.0 nM 4.87E-15 superoxide 5 2.71E-15 0.56
5 SGTC_623 k072-0108 6.24 16.9 µM 3.30E-10 heme biosynthesis & mitochondrial translocase 6 2.23E-10 0.46
6 SGTC_1044 1222-0021 6.03 1.8 µM 1.22E-9 heme biosynthesis & mitochondrial translocase 12 8.41E-10 0.24
7 SGTC_2759 disulfiram 5.90 3.4 µM 2.67E-9 8 1.87E-9 0.89
8 SGTC_2336 9001819 5.77 198.7 µM 5.55E-9 heme biosynthesis & mitochondrial translocase 23 3.93E-9 0.39
9 SGTC_1097 itavastatin 5.39 7.2 µM 4.87E-8 azole & statin 4 3.59E-8 0.31
10 SGTC_553 k007-0256 5.32 45.7 µM 6.96E-8 heme biosynthesis & mitochondrial translocase 12 5.16E-8 0.04
11 SGTC_1556 plumbagin 5.17 1.5 µM 1.56E-7 superoxide 9 1.17E-7 0.45
12 SGTC_2410 aloe-emodin-8-o-glycoside 4.98 46.3 µM 4.12E-7 mitochondrial processes 3 3.15E-7 0.84
13 SGTC_1113 artemisinin 4.94 1.2 µM 5.10E-7 heme biosynthesis & mitochondrial translocase 20 3.92E-7 0.00
14 SGTC_2766 oltipraz 4.76 83.9 µM 1.22E-6 heme requiring 13 9.49E-7 0.22
15 SGTC_227 fluconazole 4.67 33.5 µM 1.87E-6 azole & statin 39 1.47E-6 0.12
16 SGTC_562 3772-2733 4.51 112.0 µM 4.00E-6 ERG2 9 3.19E-6 0.13
17 SGTC_2930 9013230 4.50 14.9 µM 4.20E-6 heme biosynthesis & mitochondrial translocase 13 3.36E-6 0.19
18 SGTC_1326 1431-2094 4.36 10.8 µM 7.95E-6 tubulin folding & SWR complex 3 6.42E-6 0.08
19 SGTC_1453 4237-0541 4.34 195.0 µM 8.79E-6 heme biosynthesis & mitochondrial translocase 14 7.11E-6 0.36
20 SGTC_2736 butenafine 4.32 14.7 µM 9.47E-6 6 7.67E-6 0.61

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.546 2.74E-260 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.396 3.60E-126 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.395 2.11E-125 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.369 9.10E-109 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.348 6.21E-96 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.344 7.42E-94 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.342 8.97E-93 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.318 1.36E-79 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.305 3.53E-73 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.287 9.07E-65 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.280 1.50E-61 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.272 7.38E-58 YJL149W DAS1 hom Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
0.266 1.90E-55 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.266 2.83E-55 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.248 4.54E-48 YJR014W TMA22 hom Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress