YGL058W / RAD6

Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL058W / RAD6

Click on Significant Values for Screen Details ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.56|P-value:2.09E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-3.35|P-value:3.64E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.59|P-value:1.87E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:3.24|P-value:6.51E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.23|P-value:6.91E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:4.04|P-value:3.01E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.35|P-value:4.40E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-4.53|P-value:2.46E-6 ID:SGTC_1847|Compound:st056231|FD-Score:4.20|P-value:1.48E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.93|P-value:4.77E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-3.10|P-value:8.72E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-4.40|P-value:4.58E-6 ID:SGTC_2069|Compound:5228469|FD-Score:3.90|P-value:5.29E-5 ID:SGTC_2082|Compound:5219693|FD-Score:3.55|P-value:2.09E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.66|P-value:1.79E-6 ID:SGTC_2299|Compound:7976368|FD-Score:3.82|P-value:7.37E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.41|P-value:2.86E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.47|P-value:2.86E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.23|P-value:1.29E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.56|P-value:1.65E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.13|P-value:7.81E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.46|P-value:2.93E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.89|P-value:4.28E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.30|P-value:7.15E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.41|P-value:3.54E-4 ID:SGTC_3008|Compound:9078912|FD-Score:3.93|P-value:4.70E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.21|P-value:7.19E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.18|P-value:6.55E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.56|P-value:2.09E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-3.35|P-value:3.64E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.59|P-value:1.87E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:3.24|P-value:6.51E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.23|P-value:6.91E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:4.04|P-value:3.01E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.37|P-value:4.16E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.35|P-value:4.40E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-4.53|P-value:2.46E-6 ID:SGTC_1847|Compound:st056231|FD-Score:4.20|P-value:1.48E-5 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.93|P-value:4.77E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-3.10|P-value:8.72E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-4.40|P-value:4.58E-6 ID:SGTC_2069|Compound:5228469|FD-Score:3.90|P-value:5.29E-5 ID:SGTC_2082|Compound:5219693|FD-Score:3.55|P-value:2.09E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.66|P-value:1.79E-6 ID:SGTC_2299|Compound:7976368|FD-Score:3.82|P-value:7.37E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.41|P-value:2.86E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.47|P-value:2.86E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.23|P-value:1.29E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.56|P-value:1.65E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.13|P-value:7.81E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.46|P-value:2.93E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.89|P-value:4.28E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.30|P-value:7.15E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.41|P-value:3.54E-4 ID:SGTC_3008|Compound:9078912|FD-Score:3.93|P-value:4.70E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.21|P-value:7.19E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.18|P-value:6.55E-4

Top fitness defect scores for YGL058W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2163 5671233 4.66 125.0 µM 1.79E-6 10 1.59E-6
2 SGTC_2591 skatole 4.23 100.0 µM 1.29E-5 25 1.15E-5
3 SGTC_1847 st056231 4.20 15.0 µM 1.48E-5 31 1.32E-5
4 SGTC_1339 1486-1321 4.04 22.7 µM 3.01E-5 ERG2 20 2.70E-5
5 SGTC_3008 9078912 3.93 71.4 µM 4.70E-5 18 4.21E-5
6 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 3.93 62.6 µM 4.77E-5 TSC3-RPN4 17 4.28E-5
7 SGTC_2069 5228469 3.90 200.0 µM 5.29E-5 13 4.76E-5
8 SGTC_2299 7976368 3.82 114.9 µM 7.37E-5 37 6.63E-5
9 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 3.56 44.4 nM 2.09E-4 DNA damage response 36 1.89E-4
10 SGTC_2082 5219693 3.55 150.0 µM 2.09E-4 RNA processing & uracil transport 39 1.89E-4
11 SGTC_2483 5729050 3.47 27.2 µM 2.86E-4 38 2.59E-4
12 SGTC_2902 5787006 3.46 60.5 µM 2.93E-4 Golgi 40 2.66E-4
13 SGTC_2962 9082311 3.41 13.2 µM 3.54E-4 38 3.21E-4
14 SGTC_1429 4049-0205 3.37 9.5 µM 4.16E-4 25 3.78E-4
15 SGTC_1727 st034309 3.35 9.9 µM 4.40E-4 72 4.00E-4
16 SGTC_997 3996-0078 3.24 137.0 µM 6.51E-4 62 5.93E-4
17 SGTC_1190 1486-1332 3.23 13.0 µM 6.91E-4 42 6.30E-4
18 SGTC_3091 9116372 3.21 49.5 µM 7.19E-4 40 6.55E-4
19 SGTC_3179 9107801 3.09 49.5 µM 0.00111 40 0.00101
20 SGTC_3094 9117742 3.05 49.5 µM 0.00125 72 0.00115

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 3.33E-18 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.144 5.27E-17 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.128 1.01E-13 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.125 3.72E-13 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.118 7.40E-12 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.113 4.73E-11 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.113 5.45E-11 YOR097C_p YOR097C_p hom Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
0.105 9.77E-10 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.101 3.89E-9 YPL020C ULP1 het Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
0.101 4.07E-9 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.094 5.88E-8 YAR007C RFA1 het Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
0.091 1.49E-7 YDL123W SNA4 hom Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.090 1.52E-7 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.084 1.20E-6 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.082 1.76E-6 YCR091W KIN82 hom Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p