YGL074C_d

Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL074C / YGL074C

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-3.86|P-value:8.34E-5 ID:SGTC_57|Compound:miconazole|FD-Score:4.09|P-value:3.83E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.40|P-value:2.02E-16 ID:SGTC_81|Compound:0411-0046|FD-Score:4.18|P-value:2.65E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.41|P-value:4.30E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.18|P-value:9.34E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-4.77|P-value:1.62E-6 ID:SGTC_302|Compound:1094-0063|FD-Score:5.20|P-value:2.46E-7 ID:SGTC_303|Compound:1348-1668|FD-Score:3.63|P-value:2.27E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:5.91|P-value:5.27E-9 ID:SGTC_364|Compound:0083-0097|FD-Score:3.32|P-value:6.84E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.59|P-value:2.65E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-13.60|P-value:1.96E-40 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.43|P-value:3.98E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:9.99|P-value:1.77E-22 ID:SGTC_514|Compound:4466-0038|FD-Score:7.32|P-value:7.05E-13 ID:SGTC_524|Compound:r073-0010|FD-Score:3.26|P-value:8.43E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.43|P-value:4.05E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:4.87|P-value:1.25E-6 ID:SGTC_674|Compound:0498-0004|FD-Score:3.96|P-value:6.47E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:-4.40|P-value:8.72E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.49|P-value:3.82E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.90|P-value:8.23E-5 ID:SGTC_906|Compound:1165-0455|FD-Score:3.23|P-value:9.28E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.52|P-value:3.44E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.73|P-value:1.57E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.66|P-value:2.02E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.36|P-value:1.26E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.37|P-value:5.84E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:6.52|P-value:1.36E-10 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.40|P-value:5.12E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-3.86|P-value:8.32E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.36|P-value:5.13E-4 ID:SGTC_1647|Compound:st011722|FD-Score:4.51|P-value:6.42E-6 ID:SGTC_1908|Compound:st060353|FD-Score:8.31|P-value:4.16E-16 ID:SGTC_1911|Compound:st060208|FD-Score:-3.84|P-value:8.76E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-8.14|P-value:1.15E-15 ID:SGTC_2001|Compound:4023502|FD-Score:4.30|P-value:1.64E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-7.07|P-value:2.90E-12 ID:SGTC_2248|Compound:tofa|FD-Score:7.06|P-value:4.07E-12 ID:SGTC_2255|Compound:7941087|FD-Score:-3.18|P-value:9.41E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.71|P-value:2.69E-6 ID:SGTC_2363|Compound:9053361|FD-Score:4.85|P-value:1.38E-6 ID:SGTC_2368|Compound:9071271|FD-Score:3.80|P-value:1.23E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.42|P-value:4.89E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.82|P-value:1.58E-6 ID:SGTC_2652|Compound:actinonin|FD-Score:-3.40|P-value:4.48E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.48|P-value:3.33E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.30|P-value:7.32E-4 ID:SGTC_2955|Compound:9076254|FD-Score:3.23|P-value:9.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.93|P-value:7.45E-5 ID:SGTC_3207|Compound:9114163|FD-Score:4.06|P-value:4.46E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.86|P-value:8.34E-5 ID:SGTC_57|Compound:miconazole|FD-Score:4.09|P-value:3.83E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.40|P-value:2.02E-16 ID:SGTC_81|Compound:0411-0046|FD-Score:4.18|P-value:2.65E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.41|P-value:4.30E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.18|P-value:9.34E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-4.77|P-value:1.62E-6 ID:SGTC_302|Compound:1094-0063|FD-Score:5.20|P-value:2.46E-7 ID:SGTC_303|Compound:1348-1668|FD-Score:3.63|P-value:2.27E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:5.91|P-value:5.27E-9 ID:SGTC_364|Compound:0083-0097|FD-Score:3.32|P-value:6.84E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.59|P-value:2.65E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-13.60|P-value:1.96E-40 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.43|P-value:3.98E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:9.99|P-value:1.77E-22 ID:SGTC_514|Compound:4466-0038|FD-Score:7.32|P-value:7.05E-13 ID:SGTC_524|Compound:r073-0010|FD-Score:3.26|P-value:8.43E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.43|P-value:4.05E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:4.87|P-value:1.25E-6 ID:SGTC_674|Compound:0498-0004|FD-Score:3.96|P-value:6.47E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:-4.40|P-value:8.72E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.49|P-value:3.82E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.90|P-value:8.23E-5 ID:SGTC_906|Compound:1165-0455|FD-Score:3.23|P-value:9.28E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.52|P-value:3.44E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.73|P-value:1.57E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.66|P-value:2.02E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.36|P-value:1.26E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.37|P-value:5.84E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:6.52|P-value:1.36E-10 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.40|P-value:5.12E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-3.86|P-value:8.32E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.36|P-value:5.13E-4 ID:SGTC_1647|Compound:st011722|FD-Score:4.51|P-value:6.42E-6 ID:SGTC_1908|Compound:st060353|FD-Score:8.31|P-value:4.16E-16 ID:SGTC_1911|Compound:st060208|FD-Score:-3.84|P-value:8.76E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-8.14|P-value:1.15E-15 ID:SGTC_2001|Compound:4023502|FD-Score:4.30|P-value:1.64E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-7.07|P-value:2.90E-12 ID:SGTC_2248|Compound:tofa|FD-Score:7.06|P-value:4.07E-12 ID:SGTC_2255|Compound:7941087|FD-Score:-3.18|P-value:9.41E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.71|P-value:2.69E-6 ID:SGTC_2363|Compound:9053361|FD-Score:4.85|P-value:1.38E-6 ID:SGTC_2368|Compound:9071271|FD-Score:3.80|P-value:1.23E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.42|P-value:4.89E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.82|P-value:1.58E-6 ID:SGTC_2652|Compound:actinonin|FD-Score:-3.40|P-value:4.48E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.48|P-value:3.33E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.30|P-value:7.32E-4 ID:SGTC_2955|Compound:9076254|FD-Score:3.23|P-value:9.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.93|P-value:7.45E-5 ID:SGTC_3207|Compound:9114163|FD-Score:4.06|P-value:4.46E-5

Top fitness defect scores for YGL074C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_513 4245-1575 9.99 41.7 µM 1.77E-22 unfolded protein response 1 8.11E-24 1.11
2 SGTC_58 0957-0393 8.40 9.0 µM 2.02E-16 cell wall 13 2.20E-17 0.01
3 SGTC_1908 st060353 8.31 56.5 µM 4.16E-16 RPP1 & pyrimidine depletion 1 4.75E-17 1.78
4 SGTC_514 4466-0038 7.32 2.6 nM 7.05E-13 cell wall 8 1.28E-13 0.40
5 SGTC_2248 tofa 7.06 1.1 µM 4.07E-12 sphingolipid biosynthesis & PDR1 11 8.24E-13 0.00
6 SGTC_1246 0422-0085 6.52 105.0 µM 1.36E-10 1 3.42E-11 1.03
7 SGTC_360 0957-0399 5.91 1.0 µM 5.27E-9 cell wall 2 1.67E-9 0.02
8 SGTC_302 1094-0063 5.20 18.9 µM 2.46E-7 7 9.90E-8 0.05
9 SGTC_623 k072-0108 4.87 16.9 µM 1.25E-6 heme biosynthesis & mitochondrial translocase 12 5.58E-7 0.68
10 SGTC_2363 9053361 4.85 176.0 µM 1.38E-6 Golgi 6 6.19E-7 0.04
11 SGTC_2584 androsterone 4.82 48.7 µM 1.58E-6 cell wall 21 7.15E-7 0.15
12 SGTC_2348 9022543 4.71 33.4 µM 2.69E-6 mitochondrial processes 24 1.26E-6 0.14
13 SGTC_1647 st011722 4.51 22.4 µM 6.42E-6 4 3.18E-6 0.43
14 SGTC_1161 k007-0610 4.36 110.0 µM 1.26E-5 ERAD & cell cycle 33 6.48E-6 9.38E-4
15 SGTC_2001 4023502 4.30 57.7 µM 1.64E-5 mitochondrial processes 6 8.59E-6 0.22
16 SGTC_81 0411-0046 4.18 10.0 µM 2.65E-5 35 1.43E-5 0.03
17 SGTC_57 miconazole 4.09 162.5 nM 3.83E-5 ergosterol depletion effects on membrane 25 2.12E-5 0.12
18 SGTC_3207 9114163 4.06 49.5 µM 4.46E-5 10 2.49E-5 0.23
19 SGTC_674 0498-0004 3.96 451.0 µM 6.47E-5 heme requiring 21 3.70E-5 0.06
20 SGTC_2965 9082772 3.93 38.6 µM 7.45E-5 mitochondrial response to ROS 2 4.29E-5 0.68

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 6.59E-33 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.204 1.00E-32 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.186 1.87E-27 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.180 8.52E-26 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.167 2.64E-22 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.162 3.62E-21 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.162 4.00E-21 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.160 1.21E-20 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.158 2.66E-20 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.156 1.23E-19 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.153 5.48E-19 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.150 2.06E-18 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.150 2.72E-18 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.147 1.09E-17 YBL081W_p YBL081W_p hom Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport
0.147 9.31E-18 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria